PKA Implicated in the Phosphorylation of Cx43 Induced by Stimulation with FSH in Rat Granulosa Cells

Yogo, Keiichiro; Ogawa, Teiichiro; Akiyama, Motofusa; Ishida-Kitagawa, Norihiro; Sasada, Hiroshi; Sato, Eimei; Takeya, Tatsuo

doi:10.1262/jrd.17107

Cited by 48 publications

(49 citation statements)

References 45 publications

(44 reference statements)

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“…The LH surge is accompanied by an FSH surge that might be relevant to these processes [40]. It is well-known that FSH and LH exert their activities via activation of the cAMP signal [41][42][43]. Any factor that alters intracellular cAMP levels is a p o t e n t i a l m o d u l a t o r o f g r a n u l o s a c e l l differentiation and hence follicle development.…”

Section: Discussionmentioning

confidence: 99%

Changes of Cyclic AMP Levels and Phosphodiesterase Activities in the Rat Ovary

Wang

Wei

Jiang

et al. 2007

Journal of Reproduction and Development

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Abstract. Cyclic AMP (cAMP) is a second messenger that plays a critical role in follicular recruitment, development and luteinization in the mammalian ovary. The cellular level of cAMP is largely dependent on the activity of phosphodiesterase (PDE), which degrades cAMP into 5'-AMP. The present study was conducted to investigate the level of cAMP and the activity of cAMP-PDE in postnatal rats; immature rats during gonadotropin-primed follicular development, ovulation and luteinization; adult rats during normal estrous cycling; and aged rats that spontaneously developed persistent estrous (PE) by radioimmunoassay (RIA). All four rat models were confirmed by histological examination of one ovary and assayed using the other ovary by RIA. In the postnatal rats, the ovarian cAMP level was high on day 10 after birth, while ovarian cAMP-PDE activity was highest at 21 days of age. In the immature female rats, both the ovarian cAMP level and cAMP-PDE activity increased remarkably after treatment with equine chorionic gonadotropin (eCG), increased continuously 24 h after injection of human chorionic gonadotropin (hCG) for induction of ovulation and luteinization, and then declined significantly. In the adult rats during the normal estrous cycle, the ovarian cAMP levels were low on the day of estrus, and there were no significant changes in ovarian cAMP-PDE activity throughout the estrous cycle. In the PE rats, the ovarian cAMP levels were similar to those of the adult rats on the day of estrus but were lower than those on the other days of the estrous cycle; ovarian cAMP-PDE activity was lower than that in the adult rats on any day of the estrous cycle. Together, these findings indicate that the ovarian cAMP level and cAMP-PDE activity were regulated in a stage-dependent manner during ovarian follicular development, atresia and luteinization and providing evidences that cAMP and cAMP-specific PDEs are involved in these physiological processes.

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Section: Discussionmentioning

confidence: 99%

Changes of Cyclic AMP Levels and Phosphodiesterase Activities in the Rat Ovary

Wang

Wei

Jiang

et al. 2007

Journal of Reproduction and Development

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“…4B), combined with N-and C-terminal truncations and proline substitution scans to reveal the minimal interaction sites (supplementary material Fig. S3B-D (Lampe and Lau, 2004;Yogo et al, 2006), but substitutions of Ser with phosphorylated Ser at relevant positions in the proximity of the identified binding sequence did not appear to interfere with binding (supplementary material Fig. S3F).…”

Section: Identification Of Ezrin-and Cx43-binding Motifsmentioning

confidence: 99%

A PKA-ezrin-connexin 43 signaling complex controls gap junction communication and thereby trophoblast cell fusion

Pidoux

Gerbaud

Dompierre

et al. 2014

Journal of Cell Science

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Cell fusion occurs as part of the differentiation of some cell types, including myotubes in muscle and osteoclasts in remodeling bone. In the human placenta, mononuclear cytotrophoblasts in a human chorionic gonadotropin (hCG)-driven process fuse to form multinucleated syncytia that allow the exchange of nutrients and gases between the maternal and fetal circulation. Experiments in which protein kinase A (PKA) is displaced from A-kinase anchoring proteins (AKAPs), or in which specific AKAPs are depleted by siRNA-mediated knockdown, point to ezrin as a scaffold required for hCG-, cAMP-and PKA-mediated regulation of the fusion process. By a variety of immunoprecipitation and immunolocalization experiments, we show that ezrin directs PKA to a molecular complex of connexin 43 (Cx43, also known as GJA1) and zona occludens-1 (ZO-1, also known as TJP1). A combination of knockdown experiments and reconstitution with ezrin or Cx43 with or without the ability to bind to its interaction partner or to PKA demonstrate that ezrin-mediated coordination of the localization of PKA and Cx43 is necessary for discrete control of Cx43 phosphorylation and hCG-stimulated gap junction communication that triggers cell fusion in cytotrophoblasts.

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“…Protein kinase A (PKA) has been considered a main effector that responds to cAMP stimulation in many kinds of somatic cells. In fact, cAMP-PKA signaling is involved in various cellular processes, such as differentiation, proliferation, and gene expression [1,2].…”

mentioning

confidence: 99%

Evidence for Existence of cAMP-Epac Signaling in the Heads of Mouse Epididymal Spermatozoa

Amano

Lee

Goto

et al. 2007

Journal of Reproduction and Development

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Abstract. Cyclic adenosine 3',5'-monophosphate (cAMP) signaling regulates the expression of fertilizing ability in mammalian spermatozoa. Many articles indicate that this signaling is mediated mainly via protein kinase A. Recently, a guanine nucleotide exchange factor for small G protein Rap1 (an exchange protein directly activated by cAMP: Epac) was discovered as a new mediator of cAMP signaling in somatic cells. The aim of this study was to reveal the existence of cAMP-Epac signaling in mouse spermatozoa. Northern blot analysis and in situ hybridization suggested that Epac1 and Epac2 mRNAs were transcribed in the seminiferous epithelia of the testis. This shows that expression of Epac mRNAs is present in mouse testicular germ cells. Indirect immunofluorescence with specific polyclonal antibodies suggested possible co-localization of Epac1 and Rap1 proteins in the heads of epididymal spermatozoa. Moreover, treatment of epididymal spermatozoa with an Epac-specific cAMP analog, 8-pMeOPT-2'-O-Me-cAMP, induced activation of Rap1, as revealed with a commercial kit for pull-down assay. These results indicate the existence of cAMP-Epac signaling in the heads of mouse epididymal spermatozoa.

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PKA Implicated in the Phosphorylation of Cx43 Induced by Stimulation with FSH in Rat Granulosa Cells

Cited by 48 publications

References 45 publications

Changes of Cyclic AMP Levels and Phosphodiesterase Activities in the Rat Ovary

Changes of Cyclic AMP Levels and Phosphodiesterase Activities in the Rat Ovary

A PKA-ezrin-connexin 43 signaling complex controls gap junction communication and thereby trophoblast cell fusion

Evidence for Existence of cAMP-Epac Signaling in the Heads of Mouse Epididymal Spermatozoa

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