Pkn Is a novel partner of cyclin T2a in muscle differentiation

Cottone, Giuliano; Baldi, Alfonso; Palescandolo, Emanuele; Manente, Lucrezia; Penta, Roberta; Paggi, Marco G.; Luca, Antonio De

doi:10.1002/jcp.20566

Cited by 15 publications

(11 citation statements)

References 18 publications

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“…In this work, we confirmed that Ccnt2 was essential for muscle differentiation. The continuous reduction of Ccnt2 during myodifferentiation, described previously [41], suggested that Ccnt2 was important for the initiation of cellular differentiation.…”

Section: Discussionmentioning

confidence: 58%

Cyclin T2: A novel miR‐15a target gene involved in early spermatogenesis

Teng

Wang

et al. 2011

FEBS Letters

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Edited by Tamas DalmayKeywords: miR-15a Cyclin T2 Cell differentiation Spermatogenesis MicroRNA microarray a b s t r a c t MicroRNAs (miRNAs) are posttranscriptional modulators of gene expression that play important roles in various biological processes. Spermatogenesis is a highly regulated process in which diploid spermatogonia eventually differentiate into haploid spermatozoa. In this study, we identified four differentially expressed miRNAs between two premeiotic male germ cells, made predictions about their putative targets, and confirmed cyclin T2 (Ccnt2) as a direct target of miR-15a. We also report that miR-15a inhibited muscle differentiation at least in part by targeting Ccnt2, which represents a novel interaction. Subsequently, miR-15a and Ccnt2 were profiled in developing mice testes to observe their inverse correlations in the postnatal 3-week period to understand their roles in spermatogenesis.

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Section: Discussionmentioning

confidence: 58%

Cyclin T2: A novel miR‐15a target gene involved in early spermatogenesis

Teng

Wang

et al. 2011

FEBS Letters

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“…Cells were collected at 24, 48, and 72 h and immediately lysed in lysis buffer as described previously (Cottone et al, 2006).…”

Section: Myogenic Differentiation Of Clonesmentioning

confidence: 99%

OPLA scaffold, collagen I, and horse serum induce a higher degree of myogenic differentiation of adult rat cardiac stem cells

Felice

Ardizzone

Luca

et al. 2009

Journal Cellular Physiology

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In the last few years, a major goal of cardiac research has been to drive stem cell differentiation to replace damaged myocardium. Several research groups have attempted to differentiate potential cardiac stem cells (CSCs) using bi- or three-dimensional systems supplemented with growth factors or molecules acting as differentiating substances. We hypothesize that these systems failed to induce a complete differentiation because they lacked an architectural space. In the present study, we isolated a pool of small proliferating and fibroblast-like cells from adult rat myocardium. The phenotype of these cells was assessed and the characterized cells were cultured in a collagen I/OPLA scaffold with horse serum to obtain fine myocardial differentiation. C-Kit(POS)/Sca-1(POS) CSCs fully differentiated in vitro when an environment more similar to the CSC niche was created. These experiments demonstrated an important model for the study of the biology of CSCs and the biochemical pathways that lead to myocardial differentiation. The results pave the way for a new surgical approach.

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“…1 In addition to the Rho GTPases, several proteins have been identified that interact with PKN family members. These include signaling proteins such as p38 MAP kinases, MKK3, MKK6, MLTKa, PDK1, MEKK2, Akt, phospholipase D, NCK, Graf/Graf2, and cyclin T2a (Quilliam et al 1996;Flynn et al 2000;Koh et al 2000;Sun et al 2000;Oishi et al 2001;Shibata et al 2001;Takahashi et al 2003Cottone et al 2006; transcription factors such as the androgen receptor, NRDF/ NeuroD2, and PCD17 (Takanaga et al 1998;Shibata et al 1999;Metzger et al 2003); and cytoskeletal proteins such as neurofilament protein, vimentin, and a-actinin (Mukai et al 1996bMatsuzawa et al, 1997). However, in most cases, the physiological significance of these interactions has not been determined.…”

Section: T He Rho Family Small Gtpases Play a Fundamentalmentioning

confidence: 99%

A Rho-Binding Protein Kinase C-Like Activity Is Required for the Function of Protein Kinase N in Drosophila Development

Betson

Settleman

2007

Genetics

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The Rho GTPases interact with multiple downstream effectors to exert their biological functions, which include important roles in tissue morphogenesis during the development of multicellular organisms. Among the Rho effectors are the protein kinase N (PKN) proteins, which are protein kinase C (PKC)-like kinases that bind activated Rho GTPases. The PKN proteins are well conserved evolutionarily, but their biological role in any organism is poorly understood. We previously determined that the single Drosophila ortholog of mammalian PKN proteins, Pkn, is a Rho/Rac-binding kinase essential for Drosophila development. By performing “rescue” studies with various Pkn mutant constructs, we have defined the domains of Pkn required for its role during Drosophila development. These studies suggested that Rho, but not Rac binding is important for Pkn function in development. In addition, we determined that the kinase domain of PKC53E, a PKC family kinase, can functionally substitute for the kinase domain of Pkn during development, thereby exemplifying the evolutionary strategy of “combining” functional domains to produce proteins with distinct biological activities. Interestingly, we also identified a requirement for Pkn in wing morphogenesis, thereby revealing the first postembryonic function for Pkn.

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Pkn Is a novel partner of cyclin T2a in muscle differentiation

Cited by 15 publications

References 18 publications

Cyclin T2: A novel miR‐15a target gene involved in early spermatogenesis

Cyclin T2: A novel miR‐15a target gene involved in early spermatogenesis

OPLA scaffold, collagen I, and horse serum induce a higher degree of myogenic differentiation of adult rat cardiac stem cells

A Rho-Binding Protein Kinase C-Like Activity Is Required for the Function of Protein Kinase N in Drosophila Development

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