PLA/HA Multiscale Nano-/Micro-Hybrid 3D Scaffolds Provide Inductive Cues to Stems Cells to Differentiate into an Osteogenic Lineage

Ayariga, Joseph A.; Dean, Morgan; Nyairo, Elijah; Thomas, Vinoy; Dean, Derrick

doi:10.1007/s11837-021-04912-7

Cited by 3 publications

(1 citation statement)

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“…The QPCR process was carried out using Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). A similar method has been employed in the analysis of genes expressed in other scaffolds [ 125 , 126 ]. Comparisons of target gene expression in the samples were carried out using the cycle threshold (Ct) normalized to the housekeeping gene GAPDH.…”

Section: Methodsmentioning

confidence: 99%

Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration

2022

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Articular cartilage defects, and subsequent degeneration, are prevalent and account for the poor quality of life of most elderly persons; they are also one of the main predisposing factors to osteoarthritis. Articular cartilage is an avascular tissue and, thus, has limited capacity for healing and self-repair. Damage to the articular cartilage by trauma or pathological causes is irreversible. Many approaches to repair cartilage have been attempted with some potential; however, there is no consensus on any ideal therapy. Tissue engineering holds promise as an approach to regenerate damaged cartilage. Since cell adhesion is a critical step in tissue engineering, providing a 3D microenvironment that recapitulates the cartilage tissue is vital to inducing cartilage regeneration. Decellularized materials have emerged as promising scaffolds for tissue engineering, since this procedure produces scaffolds from native tissues that possess structural and chemical natures that are mimetic of the extracellular matrix (ECM) of the native tissue. In this work, we present, for the first time, a study of decellularized scaffolds, produced from avian articular cartilage (extracted from Gallus Gallus domesticus), reseeded with human chondrocytes, and we demonstrate for the first time that human chondrocytes survived, proliferated and interacted with the scaffolds. Morphological studies of the decellularized scaffolds revealed an interconnected, porous architecture, ideal for cell growth. Mechanical characterization showed that the decellularized scaffolds registered stiffness comparable to the native cartilage tissues. Cell growth inhibition and immunocytochemical analyses showed that the decellularized scaffolds are suitable for cartilage regeneration.

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Section: Methodsmentioning

confidence: 99%