Placental expression of AChE, α7nAChR and NF-κB in patients with preeclampsia

Zheng, Lei; Shi, Lei; Zhou, Zhongyi; Chen, Xiaoju; Wang, Li; Lu, Zhanbin; Tang, Rong

doi:10.5603/gp.a2018.0043

Cited by 16 publications

(18 citation statements)

References 26 publications

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“…The present study for the first time linked α7nAChR downregulation in circulatory monocytes to pregnancy-induced hypertension. This result is different from previous studies which demonstrated that α7nAChR was upregulated in the placental tissue of women with preeclampsia [21–23]. This difference suggests that the change of α7nAChR expression in preeclampsia is tissue specific.…”

Section: Discussioncontrasting

confidence: 99%

“…In pregnant rats, activation of α7nAChR by nicotine mitigated lipopolysaccharide (LPS)-induced placental inflammation and symptoms mimicking preeclampsia likely through suppressing the NF-κB p65 pathway [20]. Previous studies demonstrated that expression of α7nAChR in the placenta was increased in pregnant women with preeclampsia compared with nonpregnant women [21–23]. These findings suggest that expression of α7nAChR may be associated with placental inflammation and the development of preeclampsia.…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of α7 nicotinic acetylcholine receptors in peripheral blood monocytes is associated with enhanced inflammation in preeclampsia

Shi

et al. 2019

BMC Pregnancy Childbirth

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Background Preeclampsia is associated with chronic inflammation. The cholinergic anti-inflammatory pathway regulates systemic inflammation through activating α7 nicotinic acetylcholine receptors (α7nAChR) expressed in monocytes/macrophages. This study aimed to investigate the role of α7nAChR in peripheral blood monocytes in preeclampsia. Methods Peripheral blood monocytes were isolated from 30 nonpregnant (NP), 32 normotensive pregnant (NT), and 35 preeclamptic (PE) women. Results We found that both protein and mRNA expression levels of α7nAChR in monocytes from the PE women were significantly lower than those of the NP and NT women (both p < 0.01). α7nAChR protein expression levels in monocytes were negatively correlated with levels of systolic blood pressure (r = − 0.40, p = 0.04), proteinuria (r = − 0.54, p < 0.01), tumor necrosis factor-alpha (TNF-α, r = − 0.42, p = 0.01), and interleukin (IL)-1β (r = − 0.56, p < 0.01), while positively correlated with IL-10 levels (r = 0.43, p = 0.01) in the PE women. Both baseline and lipopolysaccharides (LPS)-induced increase of TNF-α, IL-1β, and IL-6 levels from monocytes were higher in the PE group than the NP and NT groups (all p < 0.01), but IL-10 levels in the PE group was lower than that of the NP and NT groups ( p < 0.01). In addition, the NF-κB activity in monocytes from the PE women was higher than the NP and NT women ( p < 0.01). Importantly, activation of α7nAChR with its agonist PNU-282987 inhibited NF-κB, decreased TNF-α, IL-1β, and IL-6 release, and increased IL-10 release in monocytes from the PE women (all p < 0.01). Conclusion In conclusion, these findings suggest that downregulation of α7nAChR may be associated with the development of preeclampsia through increasing pro-inflammatory and decreasing anti-inflammatory cytokine release via the NF-κB pathway.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Downregulation of α7 nicotinic acetylcholine receptors in peripheral blood monocytes is associated with enhanced inflammation in preeclampsia

Shi

et al. 2019

BMC Pregnancy Childbirth

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“…The α7 nicotinic acetylcholine receptor (nAChR) protein is expressed in the central and peripheral nervous system (CNS), muscle, lung, and placenta. The nAChR signaling is associated with nicotine addiction, cancers of lung and liver, and preeclampsia (61, 62). In the brain, activation of α7nAChR in macrophages inhibits production of inflammatory cytokines (63).…”

Section: Introductionmentioning

confidence: 99%

Emerging Role of Lymphocyte Antigen-6 Family of Genes in Cancer and Immune Cells

Upadhyay

2019

Front. Immunol.

106

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Stem Cell Antigen-1 ( Sca-1/Ly6A ) was the first identified member of the Lymphocyte antigen-6 ( Ly6 ) gene family. Sca-1 serves as a marker of cancer stem cells and tissue resident stem cells in mice. The Sca-1 gene is located on mouse chromosome 15. While a direct homolog of Sca-1 in humans is missing, human chromosome 8—the syntenic region to mouse chromosome 15—harbors several genes containing the characteristic domain known as LU domain. The function of the LU domain in human LY6 gene family is not yet defined. The LY6 gene family proteins are present on human chromosome 6, 8, 11, and 19. The most interesting of these genes are located on chromosome 8q24.3, a frequently amplified locus in human cancer. Human LY6 genes represent novel biomarkers for poor cancer prognosis and are required for cancer progression in addition to playing an important role in immune escape. Although the mechanism associated with these phenotype is not yet clear, it is timely to review the current literature in order to address the critical need for future advancements in this field. This review will summarize recent findings which describe the role of human LY6 genes— LY6D, LY6E, LY6H, LY6K, PSCA, LYPD2, SLURP1, GML, GPIHBP1 , and LYNX1 ; and their orthologs in mice at chromosome 15.

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“…It has been reported that PTGES3 may be involved in steroid synthesis and metabolism signals; abnormal expression of PTGES3 can lead to disordered steroid synthesis and metabolism, and thus to the development of PE ( 56 ). In addition, it has been reported that UQCRC2 is involved in carcinoma cell signaling; it is of note that carcinoma signaling pathways, such as Notch, RhoA/ROCK and NF-κB, are also related to PE ( 60 – 62 ). Abnormal expression of UQCRC2 can result in disorders of carcinoma signaling and also possibly the occurrence of PE.…”

Section: Discussionmentioning

confidence: 99%

Identifying key genes and drug screening for preeclampsia based on gene expression profiles

et al. 2020

Oncol Lett

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Preeclampsia (PE) is characterized by gestational hypertension and proteinuria, and is a leading cause of maternal death and perinatal morbidity globally. Although the exact cause of PE remains unclear, several studies have suggested a role for abnormal expression of multiple genes. The aim of the present study was to identify key genes and related pathways, and to screen for drugs that regulate these genes for potential PE therapy. The GSE60438 dataset was acquired from the Gene Expression Omnibus database to analyze differentially expressed genes (DEGs). By constructing a protein-protein interaction network and performing reverse transcription-quantitative PCR verification, proteasome 26S subunit, non-ATPase 14, prostaglandin E synthase 3 and ubiquinol-cytochrome c reductase core protein 2 were identified as key genes in PE. In addition, PE was found to be associated with 'circadian rhythm', 'fatty acid metabolism', 'DNA damage response detection of DNA damage', 'regulation of DNA repair' and 'endothelial cell development'. Through connectivity map analysis of DEGs, furosemide and droperidol were suggested to be therapeutic drugs that may target the hub genes for PE treatment. Results analysis of GSEA were included in the discussion section of this article. In conclusion, the current study identified novel key genes associated with the onset of PE and potential drugs for PE treatment.

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Placental expression of AChE, α7nAChR and NF-κB in patients with preeclampsia

Abstract: Abnormal expression of AChE, α7nAChR and NF-κB in placenta may be associated with preeclampsia. Cho-linergic anti-inflammatory pathway may play an important role in the pathogenesis of preeclampsia.

Cited by 16 publications

References 26 publications

Downregulation of α7 nicotinic acetylcholine receptors in peripheral blood monocytes is associated with enhanced inflammation in preeclampsia

Downregulation of α7 nicotinic acetylcholine receptors in peripheral blood monocytes is associated with enhanced inflammation in preeclampsia

Emerging Role of Lymphocyte Antigen-6 Family of Genes in Cancer and Immune Cells

Identifying key genes and drug screening for preeclampsia based on gene expression profiles

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