Placental expression of endothelial constitutive nitric oxide synthase mRNA in pregnancy complicated by preeclampsia

Nasiell, Josefine; Nisell, Henry; Blanck, Agneta; Lunell, N. O.; Faxén, Margareta

doi:10.1034/j.1600-0412.1998.770503.x

Cited by 11 publications

(11 citation statements)

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“…However, they observed a lower activity of endothelial nitric oxide synthase where the uterine and placental vessels meet and increased uterine artery resistance in preeclamptic women compared to healthy pregnant women. Nasiell et al and Schiessl et al found significantly increased placental expression of endothelial nitric oxide synthase in pregnancies complicated by preeclampsia [37,38]. …”

Section: Discussionmentioning

confidence: 99%

A comparison of maternal serum levels of endothelial nitric oxide synthase, asymmetric dimethylarginine, and homocysteine in normal and preeclamptic pregnancies

Laskowska

Laskowska²,

Terbosh³

et al. 2013

Med Sci Monit

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BackgroundThe aim of this study was to determine the maternal serum concentrations of eNOS, ADMA, and homocysteine in preeclamptic pregnancies.Material/MethodsThe study was carried out on 62 patients with pregnancy complicated by early onset and 53 patients with late onset preeclampsia. The control group consisted of 65 healthy normotensive pregnant patients. The serum eNOS, ADMA and homocysteine concentrations were determined using ELISA assays.ResultsOur study revealed elevated levels of homocysteine and ADMA in the serum of women with preeclampsia. The highest levels were observed in patients with early onset preeclampsia, but the differences between both groups of preeclamptic patients with early and late onset of preeclampsia were not statistically significant. Both groups of preeclamptic women had slightly lower levels of maternal serum endothelial nitric oxide synthase than in normotensive pregnant women, but these differences were not statistically significant.ConclusionsThe higher levels of homocysteine and ADMA observed in patients with early onset preeclampsia may suggest that higher levels of maternal serum homocysteine and ADMA correlate with the severity, and may determine the earlier clinical onset of the disease. The elevated levels of ADMA and the unchanged levels of eNOS in preeclamptic pregnancies suggest that NO deficiency in this pregnancy disorder results not from a reduced level or activity of eNOS, but from elevated levels of ADMA, an endogenous eNOS inhibitor. The lowering of increased levels of homocysteine and ADMA may be helpful in therapy of vascular disturbances occurring in preeclampsia.

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Section: Discussionmentioning

confidence: 99%

A comparison of maternal serum levels of endothelial nitric oxide synthase, asymmetric dimethylarginine, and homocysteine in normal and preeclamptic pregnancies

Laskowska

Laskowska²,

Terbosh³

et al. 2013

Med Sci Monit

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“…It has also been reported that eNOS plays a significant, pathological role in the manifestation of preeclampsia (PE), the proteinuric and/or edematous form of HP [Seligman et al, 1994; Lyall et al, 1995; Nasiell et al, 1998; Norris et al, 1999]. Although circulating levels of nitrite and the oxidative production of NO are decreased in patients with PE [Seligman et al, 1994], total nitrate levels are increased in the fetoplacental, uteroplacental, and peripheral circulation [Lyall et al, 1995; Norris et al, 1999] as a result of the increased placental expression of eNOS mRNA in PE [Nasiell et al, 1998]. These increases might be part of a compensatory mechanism in the disturbed uterine circulation seen in PE [Nasiell et al, 1998; Norris et al, 1999].…”

Section: Introductionmentioning

confidence: 99%

“…Although circulating levels of nitrite and the oxidative production of NO are decreased in patients with PE [Seligman et al, 1994], total nitrate levels are increased in the fetoplacental, uteroplacental, and peripheral circulation [Lyall et al, 1995; Norris et al, 1999] as a result of the increased placental expression of eNOS mRNA in PE [Nasiell et al, 1998]. These increases might be part of a compensatory mechanism in the disturbed uterine circulation seen in PE [Nasiell et al, 1998; Norris et al, 1999]. Recently, a missense variant in exon 7 of the eNOS gene ( NOS3 ) that encodes an aspartic acid (Asp298) instead of a glutamine (Glu298) at residue 298 was identified [Yoshimura et al, 1998].…”

Section: Introductionmentioning

confidence: 99%

Endothelial nitric oxide synthase gene (NOS3) variant and hypertension in pregnancy

et al. 2001

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Hypertension in pregnancy (HP), including preeclampsia, is known to be a multifactorial disease. Recently, a Glu298Asp variant of the endothelial nitric oxide synthase gene (NOS3) was identified as being associated with coronary spasm and myocardial infarction, whereas it has been reported that endothelial nitric oxide synthase plays a role in HP. We therefore performed an association study of the Glu298Asp variant with HP among 152 HP patients and 335 normal pregnant control individuals, in the context of other risk factors before pregnancy. The frequency of the variant GA+AA NOS3 genotypes was significantly higher in the patients (0.23) than in the controls (0.12) (P < 0.01). Multivariate analysis revealed that family history of hypertension, TT genotype of the angiotensinogen gene (AGT), GA+AA NOS3 genotype, and prepregnancy body mass index > or = 24 were independent potent risk factors, after adjustment for maternal age and parity. The odds ratios of the factors were 2.7, 2.3, 2.2, and 2.1, respectively. Our results suggested that the Asp298 of NOS3 is a potent, independent risk factor for HP.

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“…It has also been reported that NOS3 plays a significant pathological role in the manifestation of PE. [14][15][16][17] A variant of the NOS3 in which glutamine (Glu298) is substituted for aspartic acid (Asp298) at residue 298 was reported to be significantly associated with HP, including PE, 5 after significant associations of this variant with coronary spasm 18 and myocardial infarction 19,20 had been reported.…”

Section: Patientsmentioning

confidence: 99%

Association between a Variant of the Glutathione S-Transferase P1 Gene (GSTP1) and Hypertension in Pregnancy in Japanese: Interaction with Parity, Age, and Genetic Factors

Ohta

Kobashi²,

Hata³

et al. 2003

Semin Thromb Hemost

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Hypertension in pregnancy (HP), including preeclampsia (PE), is known to be a multifactorial disease. Recently, an Ile105Val variant of the glutathione S-transferase P1 gene ( GSTP1) was shown to be associated with PE in The Netherlands. We therefore performed an association study of the Ile105Val variant comparing 131 patients with HP and 327 normal pregnant controls in Japan. We analyzed the data in the context of other risk factors before pregnancy. The frequency of the Ile/Val+Val/Val genotype of the GSTP1 was not significantly different between the HP (26%) patients and the controls (28%). However, in primiparous patients, the frequency was significantly different in elderly pregnancy (63% in severe HP vs. 18% in controls; P < 0.05), in the subgroup with the MM+MT genotypes of the angiotensinogen gene (50% in severe HP vs. 26% in controls; P < 0.05), and in the subgroup with the GA+AA genotypes of the endothelial nitric oxide synthase gene (42% in severe HP vs. 13% in controls; P < 0.05). These results suggest that this variant of the GSTP1 may play a role in the manifestation of HP together with other independently and/or synergistically acting factors, particularly in primiparous pregnancy.

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Placental expression of endothelial constitutive nitric oxide synthase mRNA in pregnancy complicated by preeclampsia

Abstract: Our findings indicate that the increased placental expression of ecNOS mRNA might reflect a compensatory mechanism in the disturbed uterine circulation seen in PE and/or SGA.

Cited by 11 publications

References 0 publications

A comparison of maternal serum levels of endothelial nitric oxide synthase, asymmetric dimethylarginine, and homocysteine in normal and preeclamptic pregnancies

A comparison of maternal serum levels of endothelial nitric oxide synthase, asymmetric dimethylarginine, and homocysteine in normal and preeclamptic pregnancies

Endothelial nitric oxide synthase gene (NOS3) variant and hypertension in pregnancy

Association between a Variant of the Glutathione S-Transferase P1 Gene (GSTP1) and Hypertension in Pregnancy in Japanese: Interaction with Parity, Age, and Genetic Factors

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