Placental production and maternal serum and urine levels of inhibin A and activin A are modified by antihypertensive therapy in hypertensive disorders of pregnancy

Abstract

Our data indicate that antihypertensive therapy with alpha-methyldopa may have an effect on the synthesis and/or release of placental proteins in pregnancies complicated by PE and that this effect may be independent of its known antihypertensive action.

Effect of antihypertensive therapy with α‐methyldopa on uterine artery Doppler in pregnancies with hypertensive disorders

“…Overall, the main benefit of antihypertensive treatment in these women is an improvement in maternal outcomes, in particular a halving of the risk of developing severe hypertension (relative risk 0.50; 95% CI, 0.41–0.61). We have previously shown in the same population of women that α‐methyldopa therapy is associated with a fall in maternal serum and placental levels of soluble fms‐like tyrosine kinase 1, soluble endoglin39, inhibin A, activin A40 and in arterial stiffness as measured by pulse‐wave analysis41, in women presenting with pre‐eclampsia.…”

Placental Villous Expression of TNFα and IL‐10 and Effect of Oxygen Tension in Euploid Early Pregnancy Failure

“…All women were non-smokers, with normal body mass index (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30), not on any medication, and had regular menstrual cycles. The date of the last menstrual period (LMP) was used to calculate the gestational age, and women with a history of recurrent miscarriages or who did not know their LMP were excluded from the study.…”

“…28,29 Briefly, the homogenization buffer was made up of a solution of Complete Protease Inhibitor Cocktail ™ tablets (Roche Diagnostics, Mannheim, Germany) dissolved in 2 mL of redistilled water, then 48 mL of fresh 100 mM phosphate buffer (PBS) were added, as per manufacturer's instructions. 28,29 Briefly, the homogenization buffer was made up of a solution of Complete Protease Inhibitor Cocktail ™ tablets (Roche Diagnostics, Mannheim, Germany) dissolved in 2 mL of redistilled water, then 48 mL of fresh 100 mM phosphate buffer (PBS) were added, as per manufacturer's instructions.…”

Inflammatory Cytokines in Maternal Circulation and Placenta of Chromosomally Abnormal First Trimester Miscarriages

“…Cultured villous explants were homogenized for cytokine analysis. Homogenization of villous tissue was carried out as previously described by our group [20]. All cytokines/receptors concentrations in villous explants homogenates and culture-conditioned medium were normalized against weight of tissue/well as pg/gram villous tissue.…”