Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells support hematopoietic recovery of X-irradiated human CD34+ cells

Hayashi, Naoki; Takahashi, Kenji; Abe, Yoshinao; Kashiwakura, Ikuo

doi:10.1016/j.lfs.2009.02.006

Cited by 13 publications

(8 citation statements)

References 40 publications

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“…Similar results have been obtained in separate studies of UCB [ 18 ] and PL [ 19 ] in which populations enriched in CD34 + cells were used, thus indicating that this capacity is maintained in populations with more primitive immunophenotypes. We observed a synergistic effect in which the capacity of MSCs from the two sources improved in the presence of early acting cytokines (SCF, TPO, FL, and IL-6), as we had previously reported for BM-MSCs [ 7 ] and as reported by other groups using other cytokines, such as fibroblast growth factor 1 and IL-3, in CD34 + populations [ 18 , 19 , 27 , 28 ]. In the presence of cytokines, MSCs from both sources showed similar capacities to increase proliferation.…”

Section: Discussionsupporting

confidence: 81%

“…Few studies have been conducted to assess the in vitro hematological support capacities of UCB and PL. Such capacities have been evaluated separately in each source on populations enriched in CD34 + cells [ 18 , 19 , 26 , 27 ]. However, as we have shown previously, this hematopoietic population can be divided into CD34 + CD38 + Lin − and CD34 + CD38 − Lin − subpopulations, the latter of which has a greater potential for proliferation and expansion (because of their more primitive nature compared to the former subpopulation) [ 7 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

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Human Mesenchymal Stem/Stromal Cells from Umbilical Cord Blood and Placenta Exhibit Similar Capacities to Promote Expansion of Hematopoietic Progenitor Cells In Vitro

Fajardo-Orduña

Mayani

Flores-Guzmán

et al. 2017

Stem Cells International

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Mesenchymal stem/stromal cells (MSCs) from bone marrow (BM) have been used in coculture systems as a feeder layer for promoting the expansion of hematopoietic progenitor cells (HPCs) for hematopoietic cell transplantation. Because BM has some drawbacks, umbilical cord blood (UCB) and placenta (PL) have been proposed as possible alternative sources of MSCs. However, MSCs from UCB and PL sources have not been compared to determine which of these cell populations has the best capacity of promoting hematopoietic expansion. In this study, MSCs from UCB and PL were cultured under the same conditions to compare their capacities to support the expansion of HPCs in vitro. MSCs were cocultured with CD34+CD38−Lin− HPCs in the presence or absence of early acting cytokines. HPC expansion was analyzed through quantification of colony-forming cells (CFCs), long-term culture-initiating cells (LTC-ICs), and CD34+CD38−Lin− cells. MSCs from UCB and PL have similar capacities to increase HPC expansion, and this capacity is similar to that presented by BM-MSCs. Here, we are the first to determine that MSCs from UCB and PL have similar capacities to promote HPC expansion; however, PL is a better alternative source because MSCs can be obtained from a higher proportion of samples.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Human Mesenchymal Stem/Stromal Cells from Umbilical Cord Blood and Placenta Exhibit Similar Capacities to Promote Expansion of Hematopoietic Progenitor Cells In Vitro

Fajardo-Orduña

Mayani

Flores-Guzmán

et al. 2017

Stem Cells International

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“…Cytokine stimulation plays a critical role in the survival [ 7 ], proliferation [ 8 ] and differentiation [ 9 , 10 ] of HSCs. Our previous studies also showed that several kinds of cytokines led to dramatic increases in the HSPC numbers [ 31 , 32 ]. The present results revealed that ionizing irradiation induces remarkable damage and drastically reduces the clonogenic potential, even at 0.5 Gy.…”

Section: Discussionmentioning

confidence: 98%

Characteristics of human CD34⁺cells exposed to ionizing radiation under cytokine-free conditions

et al. 2015

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To clarify the mechanisms underlying radiation-induced hematopoietic stem cell death, we investigated the effects of excessive ionizing radiation on the clonogenic potential of CD34+ cells obtained from human umbilical cord blood under cytokine-free conditions. The CD34+ cells were X-ray–irradiated (up to 2 Gy) and were cultured for 0–48 h under cytokine-free conditions. At various time-points, the CD34+ cells were investigated for survival, clonogenic potential and the generation of mitochondrial superoxide. At 12 h after X-ray irradiation, the number of viable cells had decreased to ∼70–80% compared with the 0-h non-irradiated control, whereas the clonogenic potential in the X-ray–irradiated cells had decreased to ∼50%–60% compared with the 0-h non-irradiated control. Furthermore, significant generation of mitochondrial superoxide was observed at 6 h, and reached a maximum value between 12 and 24 h after X-ray irradiation. However, no significant differences were observed between non-irradiated and X-ray–irradiated cells in terms of the generation of reactive oxygen species or in the intracellular mitochondrial contents. In addition, a cDNA microarray analysis showed that the majority of the altered genes in the CD34+ cells at 6 h after X-ray irradiation were apoptosis-related genes. These results suggest the possibility that the elimination of the clonogenic potentials of CD34+ cells involves the generation of mitochondrial superoxide induced by ionizing radiation.

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“…A variety of stromal cells have been studied as matrices for ex vivo expansion of HSC, including MSCs isolated from bone marrow [80][81][82], adipose tissue [83][84][85], placenta [86], umbilical cord blood [87][88][89], umbilical cord tissue (Wharton's Jelly) [90][91][92][93][94], and fetal liver [95]. Bone marrow-derived mesenchymal stem cells have been widely used and clinical-scale UCBMNC-BMMSC in vitro co-culture procedures were well developed [96] and validated [97].…”

Section: Co-culture With Stromal Cellsmentioning

confidence: 99%

Umbilical Cord Blood Hematopoietic Stem Cell Expansion Ex Vivo

Ren¹,

Jiang²

2013

J Blood Disord Transfus 2012

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Umbilical cord blood is an attractive source of hematopoietic stem and progenitor cells in the treatment of hematologic diseases, especially in allogeneic hematopoietic cell transplantation. However, due to the low abundance of these cells, the therapeutic use of umbilical cord blood has been limited mostly to the pediatric setting. The strategies for adult umbilical cord blood transplantation have been improved, with the recent development of various approaches for expanding stem cells in vitro and enhancing their long-term homing efficiency. In this brief review, we discuss a number of strategies for stimulating the proliferation of umbilical cord blood hematopoietic stem cells in vitro, including the utility of transcription factors and growth factors (cytokine cocktails), as well as co-culturing with stromal cells. Ultimately, we make the case that improvements in umbilical cord blood stem cell expansion will be critical for enhancing transplantation engraftment efficacy and providing potential cure for hematological diseases.

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Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells support hematopoietic recovery of X-irradiated human CD34+ cells

Cited by 13 publications

References 40 publications

Human Mesenchymal Stem/Stromal Cells from Umbilical Cord Blood and Placenta Exhibit Similar Capacities to Promote Expansion of Hematopoietic Progenitor Cells In Vitro

Human Mesenchymal Stem/Stromal Cells from Umbilical Cord Blood and Placenta Exhibit Similar Capacities to Promote Expansion of Hematopoietic Progenitor Cells In Vitro

Characteristics of human CD34⁺cells exposed to ionizing radiation under cytokine-free conditions

Umbilical Cord Blood Hematopoietic Stem Cell Expansion Ex Vivo

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Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells support hematopoietic recovery of X-irradiated human CD34+ cells

Cited by 13 publications

References 40 publications

Human Mesenchymal Stem/Stromal Cells from Umbilical Cord Blood and Placenta Exhibit Similar Capacities to Promote Expansion of Hematopoietic Progenitor Cells In Vitro

Human Mesenchymal Stem/Stromal Cells from Umbilical Cord Blood and Placenta Exhibit Similar Capacities to Promote Expansion of Hematopoietic Progenitor Cells In Vitro

Characteristics of human CD34+cells exposed to ionizing radiation under cytokine-free conditions

Umbilical Cord Blood Hematopoietic Stem Cell Expansion Ex Vivo

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Product

Resources

About

Characteristics of human CD34⁺cells exposed to ionizing radiation under cytokine-free conditions