Placetins, platelet aggregation inhibitors from Streptomyces sp. Q-1043. I. Fermentation, isolation and biological properties.

Abstract

Placetins, platelet aggregation inhibitors were obtained from the culture broth of Streptomyces sp. Q-1043. These were designated placetins A, Al, B and Bl, respectively. Placetins A and B showed strong cytotoxicities against P388, L1210 and HeLa cells.

Cytotoxic Angucycline Class Glycosides from the Deep Sea Actinomycete Streptomyces lusitanus SCSIO LR32

“…Previous work had shown that this strain, when fermented using JNP1 medium, produced predominantly four aromatic amide compounds identified as toluene-2,4-dicarbamic acid dimethyl ester, toluene-2,4-dicarbamic acid diethyl ester, toluene-2,6-dicarbamic acid dimethyl ester, and toluene-2,6-dicarbamic acid diethyl ester . We found that when this strain was fermented using modified-RA medium and the extract was analyzed by HPLC-DAD-UV, an array of secondary metabolites with UV spectra characteristic of the angucycline class of antibiotics was observed. , In this paper, we report the fermentation, isolation, and structure elucidation of five new angucycline C -glycosides, grincamycins B–F ( 1 – 5 ), from S. lusitanus SCSIO LR32 along with the previously reported grincamycin ( 6 ). Moreover, we report the cytotoxicities of compounds 1 – 6 against the human cancer cell lines HepG2, SW-1990, HeLa, NCI-H460, and MCF-7 and the mouse melanoma cell line B16.…”

“…9 We found that when this strain was fermented using modified-RA medium and the extract was analyzed by HPLC-DAD-UV, an array of secondary metabolites with UV spectra characteristic of the angucycline class of antibiotics was observed. 10,11 In this paper, we report the fermentation, isolation, and structure elucidation of five new angucycline C-glycosides, grincamycins B−F (1−5), from S. lusitanus SCSIO LR32 along with the previously reported grincamycin (6). Moreover, we report the cytotoxicities of compounds 1−6 against the human cancer cell lines HepG2, SW-1990, HeLa, NCI-H460, and MCF-7 and the mouse melanoma cell line B16.…”