Planar Block Copolymer Membranes by Vesicle Spreading

Dorn, Jan; Belegrinou, Serena; Kreiter, Maximilian; Sinner, Eva‐Kathrin; Meier, Wolfgang

doi:10.1002/mabi.201000396

Cited by 42 publications

(56 citation statements)

References 45 publications

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“…Until now, this has involved the use of surface-grafting, which features very densely packed polymer chains, or vesicle fusion process, which exhibits polycationic character; both methods exhibit limitations for protein insertion21222324. In the present work, we have combined surface chemistry and the self-assembly of amphiphilic block copolymers to produce a homogenous block copolymer TSSBM by consecutive Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) transfers25.…”

Section: Resultsmentioning

confidence: 99%

Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane

Zhang

Palivan

2013

Sci Rep

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Reconstitution of membrane proteins in artificial membrane systems creates a platform for exploring their potential for pharmacological or biotechnological applications. Previously, we demonstrated amphiphilic block copolymers as promising building blocks for artificial membranes with long-term stability and tailorable structural parameters. However, the insertion of membrane proteins has not previously been realized in a large-area, stable, and solid-supported artificial membrane. Here, we show the first, preliminary model of a channel membrane protein that is functionally incorporated in a completely artificial polymer, tethered, solid-supported bilayer membrane (TSSBM). Unprecedented ionic transport characteristics that differ from previous results on protein insertion into planar, free-standing membranes, are identified. Our findings mark a change in understanding protein insertion and ion flow within natural channel proteins when inserted in an artificial TSSBM, thus holding great potential for numerous applications such as drug screening, trace analyzing, and biosensing.

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Section: Resultsmentioning

confidence: 99%

Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane

Zhang

Palivan

2013

Sci Rep

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“…The most prominent approach involves the incorporation of aquaporin into vesicles [20][21][22][23][24] and flat-sheet membranes 25 , as shown in Figure 2, with the expectation of achieving exceptional high water fluxes, similar to those obtained in biological systems. However there are still many issues to be solved.…”

Section: Introductionmentioning

confidence: 94%

Block Copolymer Membranes for Aqueous Solution Applications

2016

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Block copolymers are known for their intricate morphology. We review the state of the art of block copolymer membranes and discuss perspectives in this field. The main focus is on pore morphology tuning with a short introduction on non-porous membranes. The two main strategies for pore formation in block copolymer membranes are (i) film casting and selective block sacrifice and (ii) self-assembly and non-solvent induced phase separation (SNIPS). Different fundamental aspects involved in the manufacture of block copolymer membranes are considered, including factors affecting the equilibrium morphology in solid films, self-assembly of copolymer in solutions and macrophase separation by solvent-non-solvent exchange. Different mechanisms are proposed for different depths of the SNIPS membrane. Block copolymer membranes can be prepared with much narrower pore size distribution than homopolymer membranes. Open questions and indications of what we consider the next development steps are finally discussed. They include the synthesis and application of new copolymers and specific functionalization, adding characteristics to respond to stimuli and chemical environment, polymerization-induced phase separation, and the manufacture of organic-inorganic hybrids.3

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“…Polymyxin, a peptide, known to make cell membranes of bacteria permeable, was used to interact with a planar block copolymer bilayer, and succeeded in modifying its transport-related properties, as shown by a decrease in the electrochemical resistance of the membrane [57]. This decrease shows that both peptides interacted with the membrane and were able to increase the permeability in a manner similar to a cell membrane.…”

Section: Biopores In Polymeric Membranementioning

confidence: 99%

Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

et al. 2011

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Today, demand exists for new systems that can meet the challenges of identifying biological entities rapidly and specifically in diagnostics, developing stable and multifunctional membranes, and engineering devices at the nanometer scale. In this respect, bio-decorated membranes combine the specificity and efficacy of biological entities, such as peptides, proteins, and DNA, with stability and the opportunity to chemically tailor the properties of polymeric membranes. A smart strategy that serves to fulfill biological criteria is required, whereby polymer membranes come to mimic biological membranes and do not disturb but rather enhance the functioning and activity of a biological entity. Different approaches have been developed, exemplified by either planar or vesicular membranes, allowing insertion inside the polymer membrane or anchoring via functionalization of the membrane surface. Inspired by nature, but incorporating the strength provided by chemical design, bio-decorated polymer membranes represent a novel concept with great potential in diagnostics and therapeutics.

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Planar Block Copolymer Membranes by Vesicle Spreading

Cited by 42 publications

References 45 publications

Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane

Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane

Block Copolymer Membranes for Aqueous Solution Applications

Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

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