Planar chromatography coupled with spectroscopic techniques

Somsen, Govert W.; Morden, W. E.; Wilson, Ian D.

doi:10.1016/0021-9673(95)00296-y

Cited by 82 publications

(33 citation statements)

References 130 publications

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“…Several methods, such as ultraviolet/visible (UV/ VIS), fluorescence, DAD, mass spectrometry (MS), Fourier transform infrared (FTIR), and Raman spectroscopy have been applied for the in situ detection of analyte zones on a TLC plate [12][13][14][15], the most common of these being UV and fluorescence. In qualitative TLC, the identification of the compounds is based on either the color reactions of the separated sample zones or on the comparison of the R F values of the analyte and a standard compound after visualization under a UV lamp.…”

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confidence: 99%

“…In those cases in which the standard compounds are not available (e.g., the screening of new natural agents or combinatorial chemistry samples), the identification of unknowns has to be performed using a specific technique, such as mass spectrometric detection. The combination of TLC and MS has been a very active research area over the last few years [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. TLC-MS has most frequently been performed as an off-line process in which the sample is scraped and extracted from the plate before MS analysis [5,22,23], or is analyzed as such with the use of various in situ techniques [15, 16, 19 -21, 24 -31], the most common of these being TLC-liquid secondary ion mass spectrometry (LSIMS), TLC-fast atom bombardment (FAB), TLC-matrix-assisted laser desorption/ionization (MALDI), and TLC-surface-assisted desorption/ionization (SALDI).…”

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confidence: 99%

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Analysis of small molecules by ultra thin-layer chromatography-atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry

Salo

Salomies

Harju

et al. 2005

J. Am. Soc. Mass Spectrom.

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The feasibility of ultra thin-layer chromatography atmospheric pressure matrix-assisted laser desorption ionization mass spectrometry (UTLC-AP-MALDI-MS) has been studied in the analysis of small molecules. Because of a thinner adsorbent layer, the monolithic UTLC plates provide 10 -100 times better sensitivity in MALDI analysis than conventional high performance thin-layer chromatography (HPTLC) plates. The limits of detection down to a low picomole range are demonstrated by UTLC-AP-MALDI-MS. Other advantages of UTLC over HPTLC include faster separations and lower solvent consumption. The performances of AP-MALDI-MS and vacuum MALDI-MS have been compared in the analysis of small drug molecules directly from the UTLC plates. The desorption from the irregular surface of UTLC plates with an external AP-MALDI ion source combined with an ion trap instrument provides clearly less variation in measurements of m/z values when compared with a vacuum MALDI-time-of-flight (TOF) instrument. The performance of the UTLC-AP-MALDI-MS method has been applied successfully to the purity analysis of synthesis products produced by solid-phase parallel synthesis method. (J Am Soc Mass Spectrom 2005, 16, 906 -915)

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confidence: 99%

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confidence: 99%

Analysis of small molecules by ultra thin-layer chromatography-atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry

Salo

Salomies

Harju

et al. 2005

J. Am. Soc. Mass Spectrom.

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“…The on-line coupling between planar chromatography and mass spectrometry provides a powerful combination for the detection and identification of compounds separated by TLC. TLC combined with mass spectrometry (TLC-MS) has been reviewed by Bush (2003Bush ( , 2004, Somsen et al (1995), and Wilson (1999). Different methods are reported in literature, but the analytical principle is the same: the sample is ionized from the layer surface by means of a laser beam, under vacuum, and in the presence of an energy-buffering matrix.…”

Section: Thin Layer Chromatography/mass Spectrometry (Tlc-ms)mentioning

confidence: 98%

Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques

Tuzimski

2010

High-Performance Thin-Layer Chromatography (HPTLC)

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Sample preparation, detection, identification, and quantitative determination of biomolecules are presented in this chapter. Advantages of planar chromatography and the basic principles (chambers, sample application, and chromatogram development) are also described. Rapid detection of biomolecules plays a strategical role in their investigation. Hyphenated techniques such as planar chromatography coupled to UV diode array detection and to mass spectrometry provide on-line extensive structural information on the metabolites prior to their isolation. In this chapter, the combination of thin-layer chromatography (TLC) with biomolecules specific detection by diode array scanning (DAD), mass spectrometry (MS), nuclear magnetic resonance (NMR), and Fourier transform infrared spectroscopy (TLC-FTIR) is discussed. In the last part of the chapter, the reader will gain useful information about a recent method of planar chromatography -multidimensional planar chromatography (MD-PC) and information on application of different modes of multidimensional planar chromatography and combination of this technique with diode array detection (MD-PC-DAD) and HPLC-DAD for separation, detection, and qualitative and quantitative determination of biomolecules. Planar chromatography as a pilot technique for HPLC is also described.Biomolecules are widespread throughout the world. It is an organic molecule produced by a living organism, including large polymeric molecules such as proteins, polysaccharides, and nucleic acids as well as small molecules such as a primary metabolites, secondary metabolites, and natural products. Many sample-preparation techniques are used in biomolecule analysis; the method selected depends on the

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“…Although spectroscopic measurements have been mainly in the ultraviolet and visible portion of the electromagnetic spectrum, summaries of notable research efforts have also described quantitative measurements by infrared spectroscopy as well as by mass spectrometry [5,6].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection in Thin-Layer Chromatography (TLC): A Review and Application of Direct On-Plate Square-Wave Anodic Stripping Voltammetry for TLC

Petrovic¹,

King²,

Dewald³

1998

Electroanalysis

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The use of square-wave anodic stripping voltammetry (SWASV) as a direct on-plate detection method for thin-layer chromatography (TLC) is described. A commercially available carbon screen-printed microelectrode array, complete with a reference and auxiliary electrode, was used to detect Cd 2þ , Cu 2þ and Pb 2þ during the chromatographic separation. Detection limits are typically less than 10 ng for each analyte. A comprehensive review of all on-plate electrochemical detection methods for TLC is also provided.

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Planar chromatography coupled with spectroscopic techniques

Cited by 82 publications

References 130 publications

Analysis of small molecules by ultra thin-layer chromatography-atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry

Analysis of small molecules by ultra thin-layer chromatography-atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry

Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques

Electrochemical Detection in Thin-Layer Chromatography (TLC): A Review and Application of Direct On-Plate Square-Wave Anodic Stripping Voltammetry for TLC

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