Many cities have undergone spatial re-distributions of low-income populations from central to suburban neighborhoods over the past several decades. A potential negative impact of these trends is that low-income populations are concentrating in more automobile oriented areas and thus resulting in increased barriers to daily travel and activity participation, particularly for those who are unable to afford a private vehicle. Accordingly, the objective of this paper is to analyze the links between increasing socio-spatial inequalities, transport disadvantage, and adverse travel behaviour outcomes. This is examined first from a theoretical perspective, and second via a spatio-temporal analysis for the Toronto region from 1991 to 2016. Findings show that many suburban areas in Toronto are not only declining in socioeconomic status, but are also suffering from increased barriers to daily travel evidenced by longer commute times and decreasing activity participation rates, relative to central neighborhoods. Because of these adverse effects, this evidence further supports the need for progressive planning and policy aimed at curbing continuing trends of suburbanization of poverty while also improving levels of transport accessibility in the suburbs.