Plant acyl-CoA-binding proteins: An emerging family involved in plant development and stress responses

Du, Zhi‐Yan; Arias, Tatiana; Meng, Wei; Chye, Mee‐Len

doi:10.1016/j.plipres.2016.06.002

Cited by 72 publications

(91 citation statements)

References 198 publications

(386 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ACBPs are analogous to some ATP-binding cassette (ABC) transporters that mobilize acyl-CoA esters and PLs (Du et al, 2016). Recently, ABCG9, ABCG11, and AGCG14 were postulated to mediate the long-distance transport of sterols or sterol conjugates in the phloem sap (Le Hir et al, 2013), besides their roles in cuticle formation (Hwang et al, 2016).…”

Section: Acbp1 Links Fa Homeostasis To Sterol Productionmentioning

confidence: 99%

“…Apart from cytosolic members, there exist other plant ACBPs that also are conserved to bind acylCoA esters and PLs (Lung and Chye, 2016a). The six Arabidopsis ACBPs are grouped into four classes by size and domain architecture (Meng et al, 2011), which are linked differently to plant development and stress responses (Xiao and Chye, 2011;Du et al, 2016;Lung and Chye, 2016b). ACBP1 and its homolog, ACBP2, are targeted to the endoplasmic reticulum (ER) and the plasma membrane (Chye, 1998;Chye et al, 1999;Li and Chye, 2003).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Acyl-CoA-Binding Protein ACBP1 Modulates Sterol Synthesis during Embryogenesis

et al. 2017

Self Cite

View full text Add to dashboard Cite

Fatty acids (FAs) and sterols are primary metabolites that exert interrelated functions as structural and signaling lipids. Despite their common syntheses from acetyl-coenzyme A, homeostatic cross talk remains enigmatic. Six Arabidopsis (Arabidopsis thaliana) acyl-coenzyme A-binding proteins (ACBPs) are involved in FA metabolism. ACBP1 interacts with PHOSPHOLIPASE Da1 and regulates phospholipid composition. Here, its specific role in the negative modulation of sterol synthesis during embryogenesis is reported. ACBP1, likely in a liganded state, interacts with STEROL C4-METHYL OXIDASE1-1 (SMO1-1), a rate-limiting enzyme in the sterol pathway. Proembryo abortion in the double mutant indicated that the ACBP1-SMO1-1 interaction is synthetic lethal, corroborating with their strong promoter activities in developing ovules. Gas chromatography-mass spectrometry revealed quantitative and compositional changes in FAs and sterols upon overexpression or mutation of ACBP1 and/or SMO1-1. Aberrant levels of these metabolites may account for the downstream defect in lipid signaling. GLABRA2 (GL2), encoding a phospholipid/sterol-binding homeodomain transcription factor, was up-regulated in developing seeds of acbp1, smo1-1, and ACBP1+/2smo1-1 in comparison with the wild type. Consistent with the corresponding transcriptional alteration of GL2 targets, high-oil, low-mucilage phenotypes of gl2 were phenocopied in ACBP1+/2smo1-1. Thus, ACBP1 appears to modulate the metabolism of two important lipid classes (FAs and sterols) influencing cellular signaling.

show abstract

Section: Acbp1 Links Fa Homeostasis To Sterol Productionmentioning

confidence: 99%

mentioning

confidence: 99%

Acyl-CoA-Binding Protein ACBP1 Modulates Sterol Synthesis during Embryogenesis

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the cytosol, acyl-CoA can be captured by acyl-CoA binding proteins (ACBPs, for overview see [10]), which provide a shuttle for the subcellular distribution of acyl chains but also supply a sink for Besides the action of membrane transporters such as FAX1 (IE) and ABCA9 (ER) as well as LACS enzymes at both compartments, it is discussed that plastid to ER transfer of lipid compounds can occur via membrane contact sites. In principle, it seems likely that both membrane contacts and membrane transport are necessary for efficient transfer of lipid compounds [8].…”

Section: Plastid Fa Export To the Ermentioning

confidence: 99%

“…Excretion of lipophilic compounds across the plasma membrane via ATP-binding cassette (ABC) transporters is summarized in [1]. For non membrane-associated processes, lipid transfer and acyl-CoA binding proteins, membrane contact sites, vesicle traffic, or acyl-exchange mechanisms, we point to recent reviews [7][8][9][10][11]. However, it is noteworthy, that for FA/lipid traffic in general most likely membrane contacts as well as tight cooperation between transport and biosynthesis steps are required.…”

Section: Introductionmentioning

confidence: 99%

Plant membrane-protein mediated intracellular traffic of fatty acids and acyl lipids

Li‐Beisson¹,

Neunzig²,

Lee³

et al. 2017

Current Opinion in Plant Biology

View full text Add to dashboard Cite

In plants, de novo synthesis of fatty acids (FAs) occurs in plastids, whereas assembly and modification of acyl lipids is accomplished in the endoplasmic reticulum (ER) and plastids as well as in mitochondria. Subsequently, lipophilic compounds are distributed within the cell and delivered to their destination site. Thus, constant acyl-exchanges between subcellular compartments exist. These can occur via several modes of transport and plant membrane-intrinsic proteins for FA/lipid transfer have been shown to play an essential role in delivery and distribution. Lately, substantial progress has been made in identification and characterization of transport proteins for lipid compounds in plant organelle membranes. In this review, we focus on our current understanding of protein mediated lipid traffic between organelles of land plants.

show abstract

“…In plant seeds, fatty acids (FAs) must be thio‐esterified to Coenzyme‐A derivatives by acyl‐CoA synthase before they can be used as precursors for the biosynthesis of other lipids or stored as TAGs (Ohlrogge & Browse, ). Acyl‐CoA‐binding proteins (ACBPs), present in eukaryotes and some prokaryotes (Burton, Rose, Faergeman, & Knudsen, ; Du, Arias, Meng, & Chye, ; Lung & Chye, ; Xiao & Chye, ; Ye & Chye, ), bind acyl‐CoA esters to maintain an intracellular acyl‐CoA pool as well as transport acyl‐CoA esters in lipid metabolism (Du et al, ; Lung & Chye, ; Xiao & Chye, ; Ye & Chye, ). In plants, ACBPs have been reported to control the enzyme activities in the Kennedy pathway and affect seed oil lipid composition (Brown, Johnson, Rawsthorne, & Hills, ; Brown, Slabas, & Denton, ; Yurchenko & Weselake, ).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis cytosolic acyl‐CoA‐binding proteins function in determining seed oil composition

Guo

Haslam

et al. 2019

Plant Direct

View full text Add to dashboard Cite

As plant seed oils provide animals with essential fatty acids (FAs), genes that regulate plant lipid metabolism have been used in genetic manipulation to improve dietary seed oil composition and benefit human health. Herein, the Arabidopsis thaliana cytosolic acyl‐CoA‐binding proteins (AtACBPs), AtACBP4, AtACBP5, and AtACBP6 were shown to play a role in determining seed oil content by analysis of atacbp (atacbp4, atacbp5, atacbp6, atacbp4atacbp5, atacbp4atacbp6, atacbp5atacbp6, and atacbp4atacbp5atacbp6) seed oil content in comparison with the Col‐0 wild type (WT). Triacylglycerol (TAG) composition in electrospray ionization‐mass spectrometer (ESI‐MS) analysis on atacbp6 seed oil showed a reduction (−50%) of C58‐TAGs in comparison with the WT. Investigations on fatty acid composition of atacbp mutants indicated that 18:2‐FA accumulated in atacbp6 and 18:3‐FA in atacbp4, both at the expense of 20:1‐FA. As TAG composition can be modified by acyl editing through phosphatidylcholines (PC) and lysophosphatidylcholines (LPC), total PC and LPC content in atacbp6 mature seeds was determined and ESI‐MS analysis revealed that LPC had increased (+300%) at the expense of PC. Among all the 14 tested PC species, all (34:1‐, 34:2‐, 34:3‐, 34:4‐, 34:5‐, 34:6‐, 36:2‐, 36:3‐, 36:5‐, 36:6‐, 38:2‐, 38:3‐, and 38:4‐PCs) but 36:4‐PC were lower in atacbp6 than the WT. In contrast, all LPC species (16:0‐, 18:1‐, 18:2‐, 18:3‐, and 20:1‐LPC) examined were elevated in atacbp6. LPC abundance also increased in atacbp4atacbp5, but not atacbp4 and atacbp5. Interestingly, when LPC composition in atacbp4atacbp5 was compared with atacbp4 and atacbp5, significant differences were observed between atacbp4atacbp5 and each single mutant, implying that AtACBP4 and AtACBP5 play combinatory roles by affecting LPC (but not PC) biosynthesis. Furthermore, PC‐related genes such as those encoding acyl‐CoA:lysophphosphatidylcholine acyltransferase (LPCAT1) and phospholipase A2 alpha (PLA2α) were upregulated in atacbp6 developing seeds. A model on the role of AtACBP6 in modulating TAG through regulating LPCAT1 and PLA2α expression is proposed. Taken together, cytosolic AtACBPs appear to affect unsaturated TAG content and are good candidates for engineering oil crops to enhance seed oil composition.

show abstract

Plant acyl-CoA-binding proteins: An emerging family involved in plant development and stress responses

Cited by 72 publications

References 198 publications

Acyl-CoA-Binding Protein ACBP1 Modulates Sterol Synthesis during Embryogenesis

Acyl-CoA-Binding Protein ACBP1 Modulates Sterol Synthesis during Embryogenesis

Plant membrane-protein mediated intracellular traffic of fatty acids and acyl lipids

Arabidopsis cytosolic acyl‐CoA‐binding proteins function in determining seed oil composition

Contact Info

Product

Resources

About