Plant antimicrobial peptides: An overview about classification, toxicity and clinical applications

Lima, Adrianne Maia; Azevedo, Mayara I.G.; Sousa, Lyndefania M; Oliveira, Nayara Santos de; Andrade, Cláudia Roberta de; Freitas, Cléverson D.T.; Souza, Pedro Filho Noronha

doi:10.1016/j.ijbiomac.2022.06.043

Cited by 53 publications

(32 citation statements)

References 72 publications

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“…They can have diverse structures and action mechanisms. Their classification is based on their tridimensional structures and amino acid sequence similarity, including thionins, hevein-like peptides, defensins, knottins, stable-like peptides, snakins, lipid transfer proteins, and cyclotides [ 56 ] ( Figure 5 ).…”

Section: Antimicrobial Peptides’ Natural Sourcementioning

confidence: 99%

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An Overview of the Potentialities of Antimicrobial Peptides Derived from Natural Sources

2022

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Antimicrobial peptides (AMPs) are constituents of the innate immune system in every kind of living organism. They can act by disrupting the microbial membrane or without affecting membrane stability. Interest in these small peptides stems from the fear of antibiotics and the emergence of microorganisms resistant to antibiotics. Through membrane or metabolic disruption, they defend an organism against invading bacteria, viruses, protozoa, and fungi. High efficacy and specificity, low drug interaction and toxicity, thermostability, solubility in water, and biological diversity suggest their applications in food, medicine, agriculture, animal husbandry, and aquaculture. Nanocarriers can be used to protect, deliver, and improve their bioavailability effectiveness. High cost of production could limit their use. This review summarizes the natural sources, structures, modes of action, and applications of microbial peptides in the food and pharmaceutical industries. Any restrictions on AMPs’ large-scale production are also taken into consideration.

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Section: Antimicrobial Peptides’ Natural Sourcementioning

confidence: 99%

“…Defensins are resistant to proteolysis and are stable to variations in temperature and pH. They prevent microbial growth, trypsin, and α-amylase activities, decrease abiotic stress, and change the redox state of ascorbic acid [ 56 ].…”

Section: Antimicrobial Peptides’ Natural Sourcementioning

confidence: 99%

An Overview of the Potentialities of Antimicrobial Peptides Derived from Natural Sources

2022

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“…), mechanisms of action, and even the source from which they were isolated (either from natural sources or synthetically) ( Figure 7 ). A general form of classification is based on their physicochemical characteristics, which can be divided into four main groups: (1) α-helices, (2) β-pleated sheets, (3) those with mixed structures, and (4) those with atypical conformations [ 51 , 52 , 53 ]. The α helical structure is characterized by coiling on itself through peptide bonds and creating a type of tube.…”

Section: Antimicrobial Peptide Classificationmentioning

confidence: 99%

Bioactive Peptides against Human Apicomplexan Parasites

et al. 2022

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Apicomplexan parasites are the causal agents of different medically important diseases, such as toxoplasmosis, cryptosporidiosis, and malaria. Toxoplasmosis is considered a neglected parasitosis, even though it can cause severe cerebral complications and death in immunocompromised patients, including children and pregnant women. Drugs against Toxoplasma gondii, the etiological agent of toxoplasmosis, are highly toxic and lack efficacy in eradicating tissue cysts, promoting the establishment of latent infection and acute relapsing disease. Cryptosporidiosis has been recognized as the most frequent waterborne parasitosis in US outbreaks; anti-cryptosporidium drug discovery still faces a major obstacle: drugs that can act on the epicellular parasite. Severe malaria is most commonly caused by the progression of infection with Plasmodium falciparum. In recent years, great progress has been made in the field of antimalarial drugs and vaccines, although the resistance of P. falciparum to artemisinin has recently gained a foothold in Africa. As seen, the search for new drugs against these parasites remains a challenge. Peptide-based drugs seem to be attractive alternative therapeutic agents recently recognized by the pharmaceutical industry, as they can kill different infectious agents and modulate the immune response. A review of the experimental effects of bioactive peptides on these parasites follows, along with comments. In addition, some biological and metabolomic generalities of the parasites are reviewed to elucidate peptide mechanisms of action on Apicomplexan targets.

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“…drugs making them effective again [1]. In this scenario, antimicrobial peptides represent a notorious group of molecules that could help scientists to find quickly a way to cope with antimicrobial resistance [13]. However, natural antimicrobial peptides have presented problems in clinical trials, such as toxicity to host cells, low resistance to proteolysis, and sometimes high cost of obtention [13,14].…”

Section: Introductionmentioning

confidence: 99%

New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo-CBP<sub>3</sub>-PepI against <em>Klebsiella pneumoniae</em>

Branco¹,

Souza²,

Neto³

et al. 2022

Preprint

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Klebsiella pneumoniae is a multidrug-resistant opportunistic human pathogen related to various infections. As such, synthetic peptides have emerged as potential alternative molecules. Mo-CBP3-PepI has presented great activity against K. pneumoniae by presenting an MIC50 at a very low concentration (31.25 µg mL-1). Here, fluorescence microscopy and proteomic analysis revealed the alteration in cell membrane permeability, ROS overproduction, and protein profile of K. pneumoniae cells treated with Mo-CBP3-PepI. Mo-CBP3-PepI led to ROS overaccumulation and membrane pore formation in K. pneumoniae cells. Furthermore, the proteomic analysis highlighted changes in essential metabolic pathways. For example, after treatment of K. pneumoniae cells with Mo-CBP3-PepI, it was seen a reduction in the abundance of protein related to DNA and protein metabolism, cytoskeleton and cell wall organization, redox metabolism, regulation factors, ribosomal proteins, and resistance to antibiotics. These reductions lead to the inhibition of DNA repair, inhibition of cell wall turnover, protein turnover, and ROS accumulation leading to cell death. Our findings indicated that Mo-CBP3-PepI might have mechanisms of action against K. pneumoniae cells, mitigating the development of resistance and thus being a potent molecule to be employed in producing new drugs against K. pneumoniae infections.

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Plant antimicrobial peptides: An overview about classification, toxicity and clinical applications

Cited by 53 publications

References 72 publications

An Overview of the Potentialities of Antimicrobial Peptides Derived from Natural Sources

An Overview of the Potentialities of Antimicrobial Peptides Derived from Natural Sources

Bioactive Peptides against Human Apicomplexan Parasites

New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo-CBP<sub>3</sub>-PepI against <em>Klebsiella pneumoniae</em>

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