Plant–bacterial endophyte secondary metabolite matching: a case study

Hagaggi, Noura Sh. A.; Mohamed, Amal

doi:10.1007/s00203-020-01989-7

Cited by 14 publications

(14 citation statements)

References 38 publications

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“…The plant is also used to treat neuropsychiatric disorders in Burkina Faso (Kinda et al, 2020). The medicinal attributes of C. procera can be credited to secondary metabolites and cardiotonic substances present in the plant (Hagaggi and Mohamed, 2020;Mehmood et al, 2020). The extracts of aboveground plant parts of C. procera exhibited strong antipyretic, analgesic, antidepressant, and neuromuscular blocking activity (Mossa et al, 1991;Garabadu et al, 2019).…”

Section: Economic Importance Pharmacological Applicationsmentioning

confidence: 99%

“…Extracts from bark and leaves showed notable antibacterial potential against Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli (Mehmood et al, 2020). A broad antibacterial spectrum has been shown by extracts of both aerial parts of C. procera and its endophytic bacteria, Bacillus siamensis (Hagaggi and Mohamed, 2020). Leaf extracts of C. procera also reduce blood glucose to a significant level, thereby indicating its antihyperglycemic potential (Nadeem et al, 2019).…”

Section: Economic Importance Pharmacological Applicationsmentioning

confidence: 99%

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An Overview of the Characteristics and Potential of Calotropis procera From Botanical, Ecological, and Economic Perspectives

et al. 2021

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Calotropis procera (Aiton) Dryand. (commonly known as the apple of sodom, calotrope, and giant milkweed) is an evergreen, perennial shrub of the family Apocynaceae, mainly found in arid and semi-arid regions. It is a multipurpose plant, which can be utilized for medicine, fodder, and fuel purposes, timber and fiber production, phytoremediation, and synthesis of nanoparticles. It has been widely used in traditional medicinal systems across North Africa, Middle East Asia, and South-East Asia. At present, it is being extensively explored for its potential pharmacological applications. Several reports also suggest its prospects in the food, textile, and paper industries. Besides, C. procera has also been acknowledged as an ornamental species. High pharmacological potential and socio-economic value have led to the pantropical introduction of the plant. Morpho-physiological adaptations and the ability to tolerate various abiotic stresses enabled its naturalization beyond the introduced areas. Now, it is recognized as an obnoxious environmental weed in several parts of the world. Its unnatural expansion has been witnessed in the regions of South America, the Caribbean Islands, Australia, the Hawaiian Islands, Mexico, Seychelles, and several Pacific Islands. In Australia, nearly 3.7 million hectares of drier areas, including rangelands and Savannahs, have been invaded by the plant. In this review, multiple aspects of C. procera have been discussed including its general characteristics, current and potential uses, and invasive tendencies. The objectives of this review are a) to compile the information available in the literature on C. procera, to make it accessible for future research, b) to enlist together its potential applications being investigated in different fields, and c) to acknowledge C. procera as an emerging invasive species of arid and semi-arid regions.

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Section: Economic Importance Pharmacological Applicationsmentioning

confidence: 99%

Section: Economic Importance Pharmacological Applicationsmentioning

confidence: 99%

An Overview of the Characteristics and Potential of Calotropis procera From Botanical, Ecological, and Economic Perspectives

et al. 2021

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“…In Burkina Faso, the plant is also used to treat mental conditions [ 15 ]. Secondary metabolites and cardiotonic compounds found in C. procera are responsible for the therapeutic properties of the plant [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…Antibacterial activity was observed in extracts from the bark and leaves against Pseudomonas aeruginosa , Klebsiella pneumoniae , Escherichia coli , and Bacillus subtilis [ 17 ]. The extracts of both aerial portions of C. procera and its endophytic bacteria, Bacillus siamensis , have been demonstrated to have a broad antibacterial range [ 16 ]. C. procera leaf extracts also significantly lower blood glucose levels, demonstrating their antihyperglycemic potential [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies

et al. 2022

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Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia. In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms. Soil-extract medium supplemented with glycerol as a carbon source and starch–casein agar medium was used for isolation of actinobacteria from rhizosphere. From the plant, a total of 31 compounds were identified using gas chromatography/mass spectrometry (GC–MS). The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%). C. procera plant extract’s antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains. The plant extract of C. procera was considered significantly active against Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli, with inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively. The plant extract was considered to be a moderate inhibitor against Bacillus subtilis, with MIC ranging from 0.60–1.50 mg/mL. On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL). The 16S rRNA gene sequence analysis showed that the potential strains belonged to the genus Streptomyces. The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity was examined by evaluating chromosome abnormalities in mouse somatic cells and DNA fragmentation assays. The current study revealed that oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals significantly reduced chromosomal abnormalities as well as DNA fragmentation in a dose-dependent manner. Moreover, C. procera extract had antimicrobial and antigenotoxic effects against CP-induced genotoxicity.

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“…Endophytic bacteria are ubiquitous and it is now well established that the individual plant hosts one or more endophytes (Gouda et al 2016 ). Recently, several studies reported that endophytes and their hosts contribute partially to the metabolism of each other (Naik et al 2019 ; Hagaggi and Mohamed 2020 ) and therefore some metabolites are co-products of the endophytes and the plants (Ludwig-Müller 2015 ). For example, a recent matching of bioactive secondary metabolites (total phenolic content, flavonoids, saponins, and tannins) production was identified between Calotropis procera and its associated bacterial endophytes (Hagaggi and Mohamed 2020 ).…”

Section: Introductionmentioning

confidence: 99%

A new source of bacterial myrosinase isolated from endophytic Bacillus sp. NGB-B10, and its relevance in biological control activity

Youseif

Abdel-Fatah

Khalil

2022

World J Microbiol Biotechnol

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Plant metabolism interacts strongly with the plant microbiome. Glucosinolates, secondary metabolites synthesized by Brassica plants, are hydrolyzed by myrosinase into bioactive compounds of great importance in human health and plant protection. Compared with myrosinase from plant sources, myrosinase enzymes of microbial origin have not been extensively investigated. Therefore, seven endophytic strains corresponding to Bacillus sp. were isolated from Eruca vesicaria ssp. sativa plants that could hydrolyse glucosinolates (sinigrin) in the culture medium and showed myrosinase activity (0.08–19.92 U mL−1). The bglA myrosinase-related gene encoding the 6-phospho-β-glucosidase (GH 1) from Bacillus sp. NGB-B10, the most active myrosinase-producing bacterium, was successfully identified. Response surface methodology (RSM) was applied to statistically optimize culture conditions for myrosinase production from Bacillus sp. strain NGB-B10. The Plackett–Burman design indicated that nitrogen concentration, incubation period, and agitation speed were the significant parameters in myrosinase production. The application of the Box–Behnken design of RSM resulted in a 10.03-fold increase in enzyme activity as compared to the non-optimized culture conditions. The myrosinase was partially purified by 40% fractionation followed by SDS-PAGE analysis which yielded two subunits that had a molecular weight of 38.6 and 35.0 KDa. The purified enzyme was stable under a broad range of pH (5.5–10) and temperatures (10–65 °C). The hydrolysis products released by bacterial myrosinase from some glucosinolate extracts had higher and/or equivalent in vitro antagonistic activity against several phytopathogenic fungi compared to the nystatin (a broad-spectrum antifungal agent). This study provides original information about a new source of bacterial myrosinase and affords an optimized method to enhance myrosinase production.

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Plant–bacterial endophyte secondary metabolite matching: a case study

Cited by 14 publications

References 38 publications

An Overview of the Characteristics and Potential of Calotropis procera From Botanical, Ecological, and Economic Perspectives

An Overview of the Characteristics and Potential of Calotropis procera From Botanical, Ecological, and Economic Perspectives

Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies

A new source of bacterial myrosinase isolated from endophytic Bacillus sp. NGB-B10, and its relevance in biological control activity

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