A diallel analysis study for estimating the pattern of inheritance and heritability of oil content and quality attributes in Rapeseed (Brassica napus L.) was carried out at The department of Plant Breeding and Genetics, The University of Agriculture, Peshawar during crop season 2011-2012. Four diverse parents viz. G2, G4, G6 and G9 of Brassica napus L were used in a full diallel fashion during the 1 st year of experiment (Crop season 2010(Crop season -2011. All the parents along with their F1 hybrids were evaluated in field condition in the 2 nd year of experiment (Crop season 2011(Crop season -2012 in randomized complete block design. Differences among these lines for Oil content, Protein content, Glucosinolate content, Erucic acid and Linolenic acid were found highly significant (P ≤ 0.01), except Oleic acid for which non-significant differences were observed, so oleic acid was excluded from diallel analysis. Additive-dominance model was found fully adequate for Oil and Protein content, whereas it was partially adequate for the remaining traits under study. After diallel analysis results, highly significant additive (a) and non-additive (b) variances for characters such as Oil content, Protein content, Glucosinolate content and Erucic acid, while for Linolenic acid non-significant additive variance was observed. Estimation of genetic parameters showed higher magnitude and significance of non-additive component for all the seed quality traits except erucic acid, for which additive component was higher in magnitude. Graphical analysis showed overdominance for all of the parameters except erucic acid for which partial dominance was observed. Average degree of dominance value for Oil content (1.344), Protein content (1.636), Glucosinolate content (1.145) and Linolenic acid (4.156) also showed the importance of overdominance gene action responsible for all these studied seed quality traits, as it was greater than one, while for Erucic acid (0.803) it was lower the one showing partial dominance. For all the studied parameters, high broad sense heritability (0.70-0.96) and low narrow sense heritability (0.05-0.683) was estimated, showing the low role and magnitude of additive gene action and suggests selection for these traits in the later segregating generation/s.