Plant cell-based drug delivery enhances affordability of biologics

Daniell, Henry; Kulchar, Rachel J.; Herzog, Roland W.; Kulis, Mike; Leong, Kam W.

doi:10.1038/s41587-023-01899-1

Cited by 8 publications

(3 citation statements)

References 14 publications

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“…These consumption patterns have also been associated with undernutrition and vitamin deficiencies in children from low-income families by providing excess calories while lacking important micronutrients [ 21 , 22 ]. Since many therapeutics to treat these diseases are prohibitively expensive [ 23 , 24 ], it is critical to understand the social determinants that contribute to disease in order to prevent them.…”

Section: Introductionmentioning

confidence: 99%

Maternal–child consumption of ultra-processed foods and sugar-sweetened beverages in informal settlements in Mumbai, India

Kumar,

Kulchar,

Khadka

et al. 2023

J Health Popul Nutr

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Background The global nutrition transition is associated with increased consumption of ultra-processed snack foods and sugar-sweetened beverages (UPF/SSB), contributing to the double burden of child obesity and undernutrition. Methods This cross-sectional study describes the prevalence of maternal and child UPF/SSB consumption and the factors associated with frequent consumption in a convenience sample of 749 children ages 6 months through 6 years and their mothers participating in a community-based child oral health program in five informal settlement communities in Mumbai, India. Mothers were interviewed regarding maternal and child oral health and nutrition characteristics, including consumption of beverages and foods associated with tooth decay—milk, soda, tea with sugar, sweets, and chips/biscuits—using standardized questionnaires. Spearman correlations were used to assess for associations between various social factors and the frequency of maternal and child consumption of the five food categories. Chi-square tests were used to assess differences in child consumption patterns by age groups. Results Though reported soda consumption was low among both mothers and children, nearly 60% of children consumed sweets and chips/biscuits daily, four to five times the rate of mothers. Factors associated with children’s frequent consumption of UPF/SSB included lower maternal education level, frequent maternal consumption of UPF/SSB, greater number of household members, greater amount of money given to the child, and closer proximity to a store. Conclusion Our findings demonstrate social factors that may promote UPF/SSB consumption. The nutritional dangers of sugary drinks and non-nutritious snacks for mothers and young children should be addressed across maternal–child health, education, and social service programs. Early childhood nutrition interventions should involve the entire family and community and emphasize the need to limit children’s consumption of unhealthy foods and beverages from an early age.

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Section: Introductionmentioning

confidence: 99%

Maternal–child consumption of ultra-processed foods and sugar-sweetened beverages in informal settlements in Mumbai, India

Kumar,

Kulchar,

Khadka

et al. 2023

J Health Popul Nutr

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“…Nonetheless, we believe that plant cell based oral antigen delivery is particularly attractive for several reasons. In our design, plant cells provide bioencapsulation that protects from degradation of the antigen in the stomach, high levels of expression can be achieved in chloroplasts, and a transmucosal carrier facilitates translocation to the intestinal immune system and therefore Treg induction (34,35). In addition, freeze dried plant cells can be stored at ambient temperature for extended periods of time, circumventing the need for cold chain.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, freeze dried plant cells can be stored at ambient temperature for extended periods of time, circumventing the need for cold chain. In general, production in whole plants and delivery of plant cells substantially lower manufacturing costs by avoiding tissue culture/bioreactors and antigen purification (34,35). Finally, drugs with general immune suppressive properties are avoided, thereby increasing safety (36).…”

Section: Introductionmentioning

confidence: 99%

Distinct functions and transcriptional signatures in orally induced regulatory T cell populations

Biswas,

So,

Bertolini

et al. 2023

Front. Immunol.

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Oral administration of antigen induces regulatory T cells (Treg) that can not only control local immune responses in the small intestine, but also traffic to the central immune system to deliver systemic suppression. Employing murine models of the inherited bleeding disorder hemophilia, we find that oral antigen administration induces three CD4+ Treg subsets, namely FoxP3+LAP-, FoxP3+LAP+, and FoxP3-LAP+. These T cells act in concert to suppress systemic antibody production induced by therapeutic protein administration. Whilst both FoxP3+LAP+ and FoxP3-LAP+ CD4+ T cells express membrane-bound TGF-β (latency associated peptide, LAP), phenotypic, functional, and single cell transcriptomic analyses reveal distinct characteristics in the two subsets. As judged by an increase in IL-2Rα and TCR signaling, elevated expression of co-inhibitory receptor molecules and upregulation of the TGFβ and IL-10 signaling pathways, FoxP3+LAP+ cells are an activated form of FoxP3+LAP- Treg. Whereas FoxP3-LAP+ cells express low levels of genes involved in TCR signaling or co-stimulation, engagement of the AP-1 complex members Jun/Fos and Atf3 is most prominent, consistent with potent IL-10 production. Single cell transcriptomic analysis further reveals that engagement of the Jun/Fos transcription factors is requisite for mediating TGFβ expression. This can occur via an Il2ra dependent or independent process in FoxP3+LAP+ or FoxP3-LAP+ cells respectively. Surprisingly, both FoxP3+LAP+ and FoxP3-LAP+ cells potently suppress and induce FoxP3 expression in CD4+ conventional T cells. In this process, FoxP3-LAP+ cells may themselves convert to FoxP3+ Treg. We conclude that orally induced suppression is dependent on multiple regulatory cell types with complementary and interconnected roles.

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H‐Bond Modulation Mechanism for Moisture‐driven Bacteriostat Evolved from Phytochemical Formulation

Zhang,

Wang,

Huang

et al. 2023

Adv Funct Materials

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The development of environment‐friendly bacteriostats in humid atmosphere is designated as a crucial step pointing to sustainable antibiotic‐free alterative. Thanks to the excellent biosafety and intrinsic bacteriostatic attributes, bioactive phytochemical formulations become a fascinating substitute for traditional antibiotics; yet, it remains a challenge to deliver them toward moisture‐activated bacteriostatic application due to the unclear release mechanism and bacteriostatic behavior. Benefitting from “green” metal–organic frameworks (MOFs) evolved from natural γ‐cyclodextrin (γ‐CD) and potassium ion (K+), an intelligent moisture‐activated phytochemical formulation is developed, which is featured by grafting bacteriostatic vanillin (a specific phytochemical extracted from Rutaceae vanilla bean) into microporous structure of MOFs via ligand implantation mechanism. According to the molecular simulation docking, the dominant pattern of host–guest structure is characteristic for H‐bond with a length of 1.9 Å, beneficial for the sterling adsorption capacity. Nevertheless, under moisture exposure, the intermolecular H‐bond is disrupted for vanillin release to destroy bacterial membrane structure, accelerate protein decomposition, and especially inhibit virulence gene transcription of cfa gene in Escherichia coli and sea gene in Staphylococcus aureus, directing to upgrade the insights into the bacteriostatic potency of phytochemicals in high‐humidity circumstance.

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Plant cell-based drug delivery enhances affordability of biologics

Cited by 8 publications

References 14 publications

Maternal–child consumption of ultra-processed foods and sugar-sweetened beverages in informal settlements in Mumbai, India

Maternal–child consumption of ultra-processed foods and sugar-sweetened beverages in informal settlements in Mumbai, India

Distinct functions and transcriptional signatures in orally induced regulatory T cell populations

H‐Bond Modulation Mechanism for Moisture‐driven Bacteriostat Evolved from Phytochemical Formulation

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