Plant Cell Culture: A Critical Tool for Agricultural Biotechnology

Petolino, J. F.; Roberts, Jean L.; Jayakumar, P.

doi:10.1385/1-59259-346-1:243

Cited by 7 publications

(2 citation statements)

References 16 publications

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“…In vitro cultures mutagenic conditions may be used; plants regenerated from organ cultivation can be phenotyped, with DNA variants, calli, protoplasmic and somatic embryogenesis ( Orbović et al, 2008 ). Studies have found that SV in long-term crop regenerated plants is particularly apparent and more evident ( Petolino et al, 2003 ). In the long period culture, for example, the number of soma-clonal variants used in the regeneration of wheat has increased ( Hartmann et al, 1989 ).…”

Section: Introductionmentioning

confidence: 99%

Wheat omics: Classical breeding to new breeding technologies

Alotaibi

Alharbi

Alotaibi

et al. 2021

Saudi Journal of Biological Sciences

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Wheat is an important cereal crop, and its significance is more due to compete for dietary products in the world. Many constraints facing by the wheat crop due to environmental hazardous, biotic, abiotic stress and heavy matters factors, as a result, decrease the yield. Understanding the molecular mechanism related to these factors is significant to figure out genes regulate under specific conditions. Classical breeding using hybridization has been used to increase the yield but not prospered at the desired level. With the development of newly emerging technologies in biological sciences i.e., marker assisted breeding (MAB), QTLs mapping, mutation breeding, proteomics, metabolomics, next-generation sequencing (NGS), RNA_sequencing, transcriptomics, differential expression genes (DEGs), computational resources and genome editing techniques i.e. (CRISPR cas9; Cas13) advances in the field of omics. Application of new breeding technologies develops huge data; considerable development is needed in bioinformatics science to interpret the data. However, combined omics application to address physiological questions linked with genetics is still a challenge. Moreover, viroid discovery opens the new direction for research, economics, and target specification. Comparative genomics important to figure gene of interest processes are further discussed about considering the identification of genes, genomic loci, and biochemical pathways linked with stress resilience in wheat. Furthermore, this review extensively discussed the omics approaches and their effective use. Integrated plant omics technologies have been used viroid genomes associated with CRISPR and CRISPR-associated Cas13a proteins system used for engineering of viroid interference along with high-performance multidimensional phenotyping as a significant limiting factor for increasing stress resistance in wheat.

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Section: Introductionmentioning

confidence: 99%

Wheat omics: Classical breeding to new breeding technologies

Alotaibi

Alharbi

Alotaibi

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…and on the epigenetic level (appearance of heritable, but potentially reversible, changes in chromatin structure, DnA methylation, gene silencing or gene activation, etc.) (3,7,13). in general, genetic changes are attributed to endoreduplication, disturbances in plant cell cycle, deletions, activation of retrotransposons (5,15).…”

Section: Introductionmentioning

confidence: 99%