Plant-derived vaccines against diarrhoeal diseases

Abstract

Transgenic plant-derived vaccines offer a new strategy for the development of safe, inexpensive vaccines against diarrhoeal diseases. In animal and Phase I clinical studies, these vaccines have been safe and immunogenic without the need for a buffer or vehicle other than the plant cell. This review examines some early attempts to develop oral transgenic plant vaccines against enteric infections such as enterotoxigenic Escherichia coli infection, cholera and norovirus infection.

Expression of neutralizing epitope of porcine epidemic diarrhea virus in potato plants

“…In comparison with injected vaccine, plant-based vaccine systems have several advantages; they are cheaper, easy to administer, have longer shelf-life and are safer (Lauterslager et al, 2001). Many papers have been published, that antigens derived from various pathogens could be synthesized at high levels and in their authentic forms in plants (Arntzen, 1997;Tacket, 2004).…”

Immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants

“…Expression of foreign antigens in plants provides an efficient way to produce economical subunit vaccines, and plant-derived oral vaccines have been suggested as particularly suitable for use, especially in poorer regions, due to ease of production, handling, and administration, without risk of being contaminated with animal pathogens [28][29][30][31]. At present the VLPs of several viruses have been produced in plants successfully, for example, human papillomavirus-like particles and Norwalk VLPs in transgenic tobacco and potato plants, respectively [32][33][34]; cottontail rabbit papillomavirus-like particles in tobacco plants [35]; hepatitis B virus (HBV) VLPs in tobacco, soybean and potato plants [36,37]; rotavirus 2/6 VLPs in tomato plants [38]; recombinant HIV-1/HBV VLPs in tobacco and Arabidopsis plants [39].…”