Plant extract-based diets differently modulate immune responses and resistance to bacterial infection in striped catfish (Pangasianodon hypophthalmus)

Single or Combined Dietary Supply of Psidium guajava and Phyllanthus amarus Extracts Differentially Modulate Immune Responses and Liver Proteome in Striped Catfish (Pangasianodon hyphophthalmus)

“…The current study revealed that the increase of serum lysozyme, complement activities, as well as total immunoglobulin levels was concentration and time-dependent in extract-based diets. In agreement with our results, several studies also indicated that Pg or Pa extract-based diets positively enhanced lysozyme, complement activities and total Ig in rohu (8,9), tilapia (10), common carp (11), and striped catfish (14). After infection by E. ictaluri, the lysozyme and complement activities as well as the total Ig mostly decreased but were still significantly higher in some extract-based diets compared to those of the control.…”

“…The fresh part of Pg (leaves) and Pa (leaves, twigs) were extracted by using 96% ethanol solvent as previously described (14). The experimental diets were prepared using the extract ingredients to contain 0, 0.08, 0.2, and 0.5% of each Pg or Pa extract and their mixture (v: v) at similar concentrations ( Table 1).…”

“…The experimental diets were prepared using the extract ingredients to contain 0, 0.08, 0.2, and 0.5% of each Pg or Pa extract and their mixture (v: v) at similar concentrations ( Table 1). The basal and experimental diets were prepared following the previous study (14) and pellets of 2 mm were stored at −20 • C until use.…”

“…Only the study by Sundaram et al (12) has demonstrated that acetone and petroleum ether extracts of Pa could protect the freshwater crab Paratelphusa hydrodomous against white spot syndrome virus. Based on the results of the in vitro evaluation the capacity of 20 plant extracts to enhance the immunity of striped catfish Pangasianodon hypophthalmus (13), we previously found that five ethanol plant extracts including Pa, Pg, sensitive plant Mimosa pudica L., neem Azadirachta indica A. Juss and asthma plant Euphorbia hirta L. were potentially interesting for modulating blood parameters, immune responses and providing better protection to striped catfish against infection with the pathogenic bacteria Edwardsiella ictaluri after 8 weeks of feeding (14). The teleost immune system differs in several ways from mammals, the innate immune system being usually considered as more efficient than the one of mammals while the acquired immunity might be weaker (15).…”

Screening and comparative study ofin vitroantioxidant and antimicrobial activities of ethanolic extracts of selected Vietnamese plants

“…Nowadays, more environment friendly prophylactic and protective solutions are claimed and natural bio-active products are examined for enhancing the immune system and health status of cultivated animals. [17,18] However, in spite of a great variety of wild plants allocated in the various eco-regions of Vietnam and the concern of aquaculture farmers in using alternatives to antibiotics, the use of natural products in aquaculture is not yet popular in the country.…”