Plant food extracts and phytochemicals: Their role as Quorum Sensing Inhibitors

Truchado, Pilar; Larrosa, Mar; Castro-Ibáñez, Irene; Allende, Ana

doi:10.1016/j.tifs.2015.02.009

Cited by 79 publications

(40 citation statements)

References 76 publications

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“…Nearly since the discovery of bacterial QS, targeting it has been regarded as a potential novel strategy to manage pathogenic bacteria by interfering with their virulent traits like biofilm formation, enzymes production, swarming activity, etc. Potential approaches in this regard include discovering compounds which degrade the autoinducers, interfere with the signal receptor, inhibit the synthesis of the autoinducers or sequester the ones that have been produced [ 34 , 35 ]. Therefore, for a given phytochemical or compound to qualify as effective QS inhibitor, it must have no effect on the growth of the bacteria but should specifically inhibit the QS-related pathways (e.g.…”

Section: Discussionmentioning

confidence: 99%

Antiquorum sensing, antibiofilm formation and cytotoxicity activity of commonly used medicinal plants by inhabitants of Borabu sub-county, Nyamira County, Kenya

et al. 2017

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Productions of various bacterial traits like production of virulence factors (e.g. toxins, enzymes), biofilm formation, luminescence among others, have been known to be controlled by quorum sensing (QS), a process that is dependent on chemical signals or autoinducers (AIs). Bacteria known to rely on such AIs are known to be virulent and tend to be resistant against various antimicrobial agents. Therefore, strategies aimed at the inhibition of QS pathways, are regarded as potential novel therapies in managing bacterial virulence hence reducing their ability to induce infections in humans. In the present study, a portfolio of 25 medicinal plant extracts (ethanol 50% v/v) used in southwestern Kenya were assayed against a transformed E. coli Top 10 reporter QS strain. This biosensor responds to the exogenous addition of 3-oxo-N-hexanoyl homoserine lactone (3OC6HSL) expressing green fluorescent protein (GFP). The large majority of the screened medicinal plants seemed to exhibit toxic effects and almost none of them induced antiquorum sensing (AQS) activity. This could be the consequence of the presence of mixed compounds in the extracts. Elaeodendron buchananii Loes and Acacia gerrardii Benth extracts that seemed to show AQS activity were further proved found to possess mild AQS but with defined antimicrobial activities, and no antibiofilm formation inhibition. As a control, an E. coli pBCA9145_jtk2828::sfGFP strain that produces constitutively GFP was used and confirmed that none of the two extracts quenched the fluorescence of sfGFP. Cytotoxicity assays with mammalian MDCK cells also did indicate that the selected extracts with putative AQS activity, also reduced the cell viability. Therefore, further studies will be needed to separate and re-test the individual compounds especially from the selected two promising plants.

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Section: Discussionmentioning

confidence: 99%

Antiquorum sensing, antibiofilm formation and cytotoxicity activity of commonly used medicinal plants by inhabitants of Borabu sub-county, Nyamira County, Kenya

et al. 2017

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“…While interfering with the QS system without affecting the viability, plant extracts have been highlighted as potential quorum sensing inhibitors (Truchado et al, 2015). As EREE, 1, 2 and fraction Q were tested at 0.25 MIC, direct growth inhibitory effects on biofilm formation and QS can be excluded.…”

Section: Discussionmentioning

confidence: 99%

Attenuation of Adhesion, Biofilm Formation and Quorum Sensing ofCampylobacter jejunibyEuodia ruticarpa

et al. 2016

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Thermophilic campylobacters are a major cause of bacterial food-borne diarrhoeal disease. Adherence and biofilm formation are key elements of Campylobacter jejuni persistence in unfavourable environmental conditions. The phytochemical analysis of Euodia ruticarpa fruit ethanol solution extract (EREE) indicated that the major compounds were evodiamine (1), rutaecarpine (2) and evocarpine (9). E. ruticarpa fruit ethanol solution extract, compounds 1 and 2 as well as a mixture of quinolinone alkaloids with 41.7% of 9 were tested for antibacterial, antibiofilm and antiquorum sensing activities against C. jejuni. Minimal inhibitory concentrations varied from 64 to 1024 µg/mL. A mutant strain that lacks the functional gene coding for the CmeB efflux pump protein was the most susceptible. Interestingly, in addition to the wild-type (NCTC 11168) and cmeB mutant, also a mutant that lacks autoinducer-2 production (luxS) was able to adhere (1 h) and to produce a biofilm (24, 48 and 72 h). The subinhibitory concentrations of all preparations at least partly inhibited C. jejuni adhesion and biofilm formation with the most visible effect of the quinolinone alkaloid fraction. Using a Vibrio harveyi luminescence assay, the inhibition of autoinducer-2 production was observed in the wild-type and cmeB mutant after 48 h with the most visible effect of EREE and its fraction Q. Copyright © 2016 John Wiley & Sons, Ltd.

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“…According with CDC and NIH, between 65%-80% of infections could be caused by biofilms, demonstrating the need to develop improved treatment options [9]. Therefore, targeting and blocking QS circuits in bacteria may represent a strategy to disarm their virulence and hence, making them more susceptible to elimination by the host immune system or low doses of antibiotics [10]. Anti-virulent agents possessing QS inhibitory activity might not pose selective pressure on bacterial pathogens and hence, may contribute to reduce the rapid emergence of so-called "superbugs", bacteria resistant to several antibiotics [11,12].…”

Section: Introductionmentioning

confidence: 99%

Chitosan nanoencapsulation of flavonoids enhances their quorum sensing and biofilm formation inhibitory activities against an E.coli Top 10 biosensor

Omwenga

Hensel

Shitandi

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Phytochemicals have been found to be promising alternatives to conventional antibiotic therapies for the control of bacterial infections, as they may entail less selective pressure and hence reduce the development of resistance. This study involved examining the inhibition of biofilm formation and of quorum sensing (QS), and the cytotoxicity on mammalian cells of two flavonoids, quercetin and baicalein, in free form and associated into chitosan-based nanocapsules. This was done by use of a transformed E. coli Top 10 biosensor strain, while the cytotoxicity was evaluated on MDCK-C7 cells. In free form, application both flavonoids exhibited slight inhibitory activity on the QS response and biofilm formation, a scenario that was improved positively upon encapsulation with chitosan (Mw ∼115,000 g/mol and DA ∼42%). The association efficiency of 99% (quercetin) and 87% (baicalein) was determined, and each formulation had an average diameter of 190 ± 4 and 187 ± 2 nm, and zeta (ζ) potential of +48.1 ± 2.03 and +48.4 ± 3.46 mV, respectively. Both types of systems were stable against aggregation in M9 and MEM media. The in vitro release kinetics data of both flavonoids seemed to be similar with only ∼20% released over the first 5 h, or ∼10% over the first 4 h, respectively, with subsequent sudden release increase up to ∼40% in both cases. The free phytochemicals seemed to be cytotoxic to MDCK-C7 cells at higher doses, however, upon nanoencapsulation, a cytoprotective effect was evidenced. We have gained proof-of-principle of the advantages of encapsulation of two bioactive flavonoids.

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Plant food extracts and phytochemicals: Their role as Quorum Sensing Inhibitors

Cited by 79 publications

References 76 publications

Antiquorum sensing, antibiofilm formation and cytotoxicity activity of commonly used medicinal plants by inhabitants of Borabu sub-county, Nyamira County, Kenya

Antiquorum sensing, antibiofilm formation and cytotoxicity activity of commonly used medicinal plants by inhabitants of Borabu sub-county, Nyamira County, Kenya

Attenuation of Adhesion, Biofilm Formation and Quorum Sensing ofCampylobacter jejunibyEuodia ruticarpa

Chitosan nanoencapsulation of flavonoids enhances their quorum sensing and biofilm formation inhibitory activities against an E.coli Top 10 biosensor

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