Plant Geminivirus Rep Protein Induces Rereplication in Fission Yeast

Kittelmann, Katharina; Rau, Peter; Gronenborn, Bruno; Jeske, Holger

doi:10.1128/jvi.02491-08

Cited by 28 publications

(23 citation statements)

References 81 publications

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“…This is the opposite of the case with geminiviruses, where DNA synthesis is induced but cell division is blocked (Ascencio-Ibáñez et al, 2008;Kittelmann et al, 2009). At the cellular level, ectopic Nt upp-L overexpression pushed palisade and spongy mesophyll cells from the G1 phase to the mitotic cycle, promoting cell divisions in fully developed leaves in which cell division is normally limited.…”

Section: Overexpression Of Upp-l But Not Upp-s Results In Hyperplasiamentioning

confidence: 90%

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

et al. 2013

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Plant viruses cause a variety of diseases in susceptible hosts. The disease symptoms often include leaf malformations and other developmental abnormalities, suggesting that viruses can affect plant development. However, little is known about the mechanisms underlying virus interference with plant morphogenesis. Here, we show that a C-4 type zinc-finger (ZF) protein, p12, encoded by a carlavirus (chrysanthemum virus B) can induce cell proliferation, which results in hyperplasia and severe leaf malformation. We demonstrate that the p12 protein activates expression of a regulator of cell size and proliferation, designated upp-L (upregulated by p12), which encodes a transcription factor of the basic/helix-loop-helix family sufficient to cause hyperplasia. The induction of upp-L requires translocation of the p12 protein into the nucleus and ZF-dependent specific interaction with the conserved regulatory region in the upp-L promoter. Our results establish the role of the p12 protein in modulation of host cell morphogenesis. It is likely that other members of the conserved C-4 type ZF family of viral proteins instigate reprogramming of plant development by mimicking eukaryotic transcriptional activators.

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Section: Overexpression Of Upp-l But Not Upp-s Results In Hyperplasiamentioning

confidence: 90%

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

et al. 2013

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“…A stringent control of geminiviral replication in host tissues may be advantageous for the virus. The overexpression of Rep has been shown to induce the rereplication of host DNA in yeast (66,67), and geminiviral infection led to the same effect on plants (68), in addition to enhanced somatic homologous recombination (69). It is likely that these detrimental effects need to be minimized in order to optimize viral fitness.…”

Section: Discussionmentioning

confidence: 99%

Early Function of the Abutilon Mosaic Virus AC2 Gene as a Replication Brake

Krenz

Deuschle

Deigner

et al. 2015

J Virol

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The C2/AC2 genes of monopartite/bipartite geminiviruses of the genera Begomovirus and Curtovirus encode important pathogenicity factors with multiple functions described so far. A novel function of Abutilon mosaic virus (AbMV) AC2 as a replication brake is described, utilizing transgenic plants with dimeric inserts of DNA B or with a reporter construct to express green fluorescent protein (GFP). Their replicational release upon AbMV superinfection or the individual and combined expression of epitope-tagged AbMV AC1, AC2, and AC3 was studied. In addition, the effects were compared in the presence and in the absence of an unrelated tombusvirus suppressor of silencing (P19). The results show that AC2 suppresses replication reproducibly in all assays and that AC3 counteracts this effect. Examination of the topoisomer distribution of supercoiled DNA, which indicates changes in the viral minichromosome structure, did not support any influence of AC2 on transcriptional gene silencing and DNA methylation. The geminiviral AC2 protein has been detected here for the first time in plants. The experiments revealed an extremely low level of AC2, which was slightly increased if constructs with an intron and a hemagglutinin (HA) tag in addition to P19 expression were used. AbMV AC2 properties are discussed with reference to those of other geminiviruses with respect to charge, modification, and size in order to delimit possible reasons for the different behaviors. IMPORTANCEThe (A)C2 genes encode a key pathogenicity factor of begomoviruses and curtoviruses in the plant virus family Geminiviridae. This factor has been implicated in the resistance breaking observed in agricultural cotton production. AC2 is a multifunctional protein involved in transcriptional control, gene silencing, and regulation of basal biosynthesis. Here, a new function of Abutilon mosaic virus AC2 in replication control is added as a feature of this protein in viral multiplication, providing a novel finding on geminiviral molecular biology.

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“…Protein expression of a C4 transgene from a Cauliflower mosaic virus (CaMV) 35S (Park et al 2010) or an inducible (Mills-Lujan and Deom 2010) promoter leads to cell proliferation and developmental abnormalities. When expressed in resting yeast cells, the Rep proteins are able to stimulate them to undergo multiple rounds of DNA synthesis without mitosis (Kittelmann et al 2009), indicating that this particular signaling route is conserved among fungi and plants.…”

Section: Replicationmentioning

confidence: 97%

Virus Operation Control Centers

Melcher

2011

Biocommunication of Plants

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The nucleic acids of plant viruses are the control centers that coordinate all activities associated with virus survival and propagation within cells, in whole plants and between organisms. Within cells, the viruses use a diversity of signaling mechanisms to assure the orderly production at specific subcellular locations of viral mRNAs, viral proteins, viral genomic nucleic acids, and viral particles and the export of infectious entities to neighboring cells. Within cells, viruses also signal their presence to the host cell machinery, establishing the conditions of coexistence of virus and plant in successful infections. At the plant level, the control centers direct the movement of infectious entities from one cell to another, into the vascular system, and into tissues remote from the site of initial infection. At the same time, the control centers condition the plant to be hospitable to virus reproduction and survival. They also cause the plant to issue signals to potential vectors guiding them to the plant to acquire the virus and encouraging their departure to further plants, in effect spreading the virus among multiple plants. The signals used in these processes include small molecules (hormones and volatiles), macromolecules with binding sites for other molecules (some being enzymatic), macromolecular structure conformations, genomic organizations, and others. Often, different viruses accomplish the same activity in completely different ways, although some common strategies are employed.

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Plant Geminivirus Rep Protein Induces Rereplication in Fission Yeast

Cited by 28 publications

References 81 publications

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

An RNA Virus-Encoded Zinc-Finger Protein Acts as a Plant Transcription Factor and Induces a Regulator of Cell Size and Proliferation in Two Tobacco Species

Early Function of the Abutilon Mosaic Virus AC2 Gene as a Replication Brake

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