PLANT GROWTH OF EGGPLANT (Solanum melongena L.) IN VITRO IN DROUGHT STRESS POLYETHYLENE GLYCOL (PEG)

Siaga, Erna; Maharijaya, Awang; Rahayu, Megayani Sri

doi:10.24233/biov.2.1.2016.29

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…The high polarity of PEG raises hydrophilicity and increases its water solubility [ 14 ]. PEGs do not react with chemicals or biological compounds; they are also non-toxic, cannot be absorbed by plants, and their concentration remains constant throughout the test period [ 15 ]. Sorbitol is water-soluble polyhydric alcohol (polyols) having 0.6 sweetness from sucrose and corn syrup and is synthesized from glucose, which is naturally detected in some plants [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…Studies on the effects of drought in melons have been conducted under field and greenhouse conditions [ 3 , 7 , 17 ], and no detailed studies have been conducted under in vitro conditions for the screening and selection of C. melo genotypes, especially for the assessment of seedlings responses after germination stage. Some studies were conducted on the effects of osmotic materials by creating in vitro and in vivo drought stress in other crops [ 11 , 13 , 15 ]. Still, no related studies have been traced on the seedlings of melon in the literature so far.…”

Section: Introductionmentioning

confidence: 99%

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Polyethylene Glycol and Sorbitol-Mediated In Vitro Screening for Drought Stress as an Efficient and Rapid Tool to Reach the Tolerant Cucumis melo L. Genotypes

Mehmandar

Rasouli

Giglou

et al. 2023

Plants

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An efficient method to instantly assess drought-tolerant plants after germination is using osmoregulation in tissue culture media. In this study, the responses of three Iranian melon genotypes to sorbitol (0.1, 0.2, and 0.4 M) or polyethylene glycol (PEG) (0.009, 0.012, and 0.015 M) were evaluated as drought stress simulators in MS medium. ‘Girke’ (GIR), ‘Ghobadloo’ (GHO), and ‘Toghermezi’ (TOG) were the genotypes. GIR is reputed as a drought-tolerant genotype in Iran. The PEG or sorbitol decreased the coleoptile length, fresh weight, and photosynthetic pigments content while enhancing proline and malondialdehyde (MDA) contents. Protein content and antioxidant enzyme activity were utterly dependent on genotype, osmotic regulators, and their concentration. Coleoptile length, root and shoot fresh weight, root dry weight, proline and MDA content, and guaiacol peroxidase (GPX) activity can be used as indicators for in vitro screening of Cucumis melo L. genotypes. The results showed that sorbitol mimics drought stress better than PEG. Overall, our findings suggest that in vitro screening could be an accurate, rapid, and reliable methodology for evaluating and identifying drought-tolerant genotypes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polyethylene Glycol and Sorbitol-Mediated In Vitro Screening for Drought Stress as an Efficient and Rapid Tool to Reach the Tolerant Cucumis melo L. Genotypes

Mehmandar

Rasouli

Giglou

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…The high polarity of PEG rises hydrophilicity and increases water solubility 8 . The PEG do not react with chemicals or biologicals compounds, are non-toxic, cannot be absorbed by plants and its concentration remains constant throughout the test period 9 . Sorbitol is a water-soluble polyhydric alcohol (polyols) having 0.6 sweetness from sucrose, made from corn syrup and synthesized from glucose, and naturally detected in some plants 10 .…”

Section: Introductionmentioning

confidence: 99%

In Vitro Screening of Cucmis Melo L. Against Drought Mediated By PEG and Sorbitol

Mn¹,

Rasouli²,

Mt³

et al. 2021

Preprint

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Water deficit in first growth stages of melon (Cucumis melo L.) in formation of first true leaves after germination can be a factor limiting production. The first step for resolve the problem is genotypes evaluation and identification of drought tolerant melons. An effective method to achieve the goal is use of osmotic solution in tissue culture. Responses of Iranian melon landraces to drought was evaluated using sorbitol at 0.1, 0.2 and 0.4 M or polyethyleneglycol (PEG 6000) at 0.009, 0.012 and 0.015 M concentrations, and MS medium without treatment as the control. Coleoptile length, fresh and dry weight of shoots and roots, photosynthetic pigments, protein, proline, malondialdehyde (MDA) and antioxidant enzymes superoxide dismutase, guaiacol peroxidase and ascorbate peroxidase were measured. The PEG or sorbitol decreased coleoptile length, fresh weight and photosynthetic pigments, and led to enhancement of proline and MDA. Contents of protein and antioxidant enzymes was completely dependent on genotype and type and concentration of osmotic material. The in vitro culture for screening and identification of tolerant and sensitive drought genotypes could be rapid, useful and effective, with sorbitol mimicing drought better than PEG. After in vitro evaluation, the genotype responses to induced water deficit need to be confirmed under field conditions.

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Morphological and Physiological Responses of In Vitro-Grown Cucurbita sp. Landraces Seedlings under Osmotic Stress by Mannitol and PEG

et al. 2022

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Screening and identification of tolerant genotypes using osmotic materials under in vitro culture could be rapid, easy, and even accurate. In this research, three Iranian landraces of Cucurbita sp. included Tanbal Ajili (Cucurbita maxima Duch.), Ajili Razan (Cucurbita pepo L.), and Balghabakhi (Cucurbita moschata Duch.) seeds were cultured in ¼ MS medium. After germination, plantlets were transferred to MS media containing mannitol and PEG 6000. Mannitol and PEG at three concentrations of 0.1, 0.2, and 0.4 M and 0.009, 0.012, and 0.015 M, respectively, were added into the MS medium, while the MS medium without any adding was used as control. Our findings revealed that osmotic treatments significantly increased shoot and root dry weight (DW), malondialdehyde (MDA), and proline content, but significantly reduced coleoptile length, shoot and root fresh weight, and photosynthesis pigments content. Protein content, phenols, and flavonoids content, enzymatic and non-enzymatic antioxidant including ascorbate peroxidase (APX), guaiacol peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) activity, reduced ascorbate (AsA), reduced ascorbate/dehydroascorbic acid (AsA/DHA), reduced glutathione (GSH), dehydroascorbic acid (DHA) and oxidized glutathione (GSSG), and reduced glutathione/oxidized glutathione (GSH/GSSG) were significantly increased at moderate osmotic stress induced by mannitol and PEG. In contrast, the previous physiological parameters were significantly reduced at higher water deficit conditions. With respect to most attributes and concentrations, mannitol simulated osmotic stress better than PEG. Our results revealed that applying PEG and mannitol under in vitro conditions could be an efficient way to evaluate and screen cucurbit genotypes for future breeding programs.

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PLANT GROWTH OF EGGPLANT (Solanum melongena L.) IN VITRO IN DROUGHT STRESS POLYETHYLENE GLYCOL (PEG)

Cited by 3 publications

References 7 publications

Polyethylene Glycol and Sorbitol-Mediated In Vitro Screening for Drought Stress as an Efficient and Rapid Tool to Reach the Tolerant Cucumis melo L. Genotypes

Polyethylene Glycol and Sorbitol-Mediated In Vitro Screening for Drought Stress as an Efficient and Rapid Tool to Reach the Tolerant Cucumis melo L. Genotypes

In Vitro Screening of Cucmis Melo L. Against Drought Mediated By PEG and Sorbitol

Morphological and Physiological Responses of In Vitro-Grown Cucurbita sp. Landraces Seedlings under Osmotic Stress by Mannitol and PEG

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