<p><em>Penelitian ini bertujuan untuk mempelajari  pengaruh dari penambahan berbagai bahan organik terhadap pertumbuhan tanaman krisan secara in vitro, dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Departemen Agronomi dan Hortikultura. Hasil penelitian diolah menggunakan metode Rancangan Acak Lengkap (RAL) dengan dua faktor. Faktor pertama adalah perlakuan media bahan organik terdiri atas tujuh taraf (MS0 tanpa penambahan bahan organik, kulit pisang 50 g/l + ½ MS (½ konsentrasi hara makro, mikro dan vitamin), kulit pisang 100 g/l + ½ MS, kulit pisang 150 g/l + ½ MS, ubi jalar ungu 50 g/l + ½ MS, ubi jalar ungu 100 g/l + ½ MS dan ubi jalar ungu 150 g/l + ½ MS). Faktor kedua adalah perlakuan varietas krisan dengan dua jenis (Puspita Nusantara dan Puspita Pelangi). Hasil penelitian menunjukkan bahwa penambahan bahan organik dalam media kultur jaringan krisan memberikan pengaruh terhadap tinggi tanaman, jumlah daun, jumlah akar, dan panjang akar. Varietas krisan memberikan pengaruh pada tinggi tanaman dan  jumlah daun tanaman krisan. Interaksi antara media bahan organik dan varietas krisan terjadi pada tinggi tanaman umur 4–10 MSK.</em></p>

Penelitian ini dilakukan untuk mempelajari aspek pengelolaan budi daya komoditas kentang. Penelitian dilaksanakan di Kabupaten Bandung, Jawa Barat pada tanggal 14 Februari sampai dengan 14 Juni 2011. Aspek khusus yang diamati adalah pengaruh pembumbunan awal terhadap pertumbuhan tanaman kentang. Perlakuan yang dilakukan adalah ketinggian pembumbunan pada 10 cm, 15 cm, 20 cm dan variabel yang diamati adalah persentase pertumbuhan bibit, tinggi tanaman, jumlah stolon per batang dan bobot umbi saat panen. Hasil analisis menunjukkan bahwa tinggi perlakuan pembumbunan berpengaruh nyata terhadap persentase tumbuh bibit pada 21 hari setelah tanam dan berpengaruh sangat nyata terhadap tinggi tanaman pada 28 hari setelah tanam

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

Drought stress is one of the important issues related to the global warming that demand for the development of drought tolerant crops. Eggplant is one of the agricultural commodities which can be developed in dry land so plant growth of eggplant need to be learned. The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, and to find the drought tolerant eggplant accessions in dry land. The experiment  was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was laid on a completely randomized design with one factor. The factor was eggplant accessions. The results showed that PEG in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Callus in eggplant explants as a way of avoiding drought stress.

Abstract. Rahayu MS, Setiawan A, Maskromo I, Purwito A, Sudarsono. 2021. Genetic diversity analysis of Puan Kalianda kopyor coconuts (Cocos nucifera) from South Lampung, Indonesia based on SSR markers. Biodiversitas 23: 205-211. Puan Kalianda kopyor coconut (Cocos nucifera L.) is a newly released tall kopyor coconut from Kalianda, South Lampung, Indonesia. The kopyor coconut is an exotic, highly economic value coconut mutant with an abnormal endosperm. This study aimed to analyze the genetic diversity of Puan Kalianda kopyor coconut from South Lampung, Indonesia using SSR markers. As many as 91 Puan Kalianda kopyor coconut accessions were genotyped using 10 SSR marker loci, and the generated data were used to evaluate their genetic diversity and population structure. The results showed a high degree of SSR marker polymorphism (PIC value = 67%), indicating the SSR marker loci are informative for revealing the genetic diversity within the evaluated Puan Kalianda kopyor coconut population. The Puan Kalianda coconut population showed a 70% expected heterozygosity (He) and 60% observed heterozygosity. The phylogenetic analysis formed two main clusters, and each cluster consisted of three sub-clusters. The Genetic structure analysis showed that the population most probably derived from two ancestral origins (K = 2) and can further be clustered into six sub-clusters (K = 6). Therefore, since genetic diversity within the population is relatively high, the Puan Kalianda tall kopyor coconut population can be considered an essential genetic resource for future kopyor coconut development.