Plant growth-promoting bacteria isolated from wild legume nodules and nodules of Phaseolus vulgaris L. trap plants in central and southern Mexico

Tapia-García, Erika Yanet; Hernández-Trejo, Verónica; Guevara-Luna, Joseph; Rojas-Rojas, Fernando Uriel; Arroyo-Herrera, Ivan; Meza-Radilla, Georgina; Vásquez-Murrieta, María Soledad; Santos, Paulina Estrada‐de los

doi:10.1016/j.micres.2020.126522

Cited by 43 publications

(29 citation statements)

References 71 publications

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“…Specifically, we tested for bacteria that (i) promoted plant growth in N-limiting substrates, (ii) solubilized phosphate, (iii) produced siderophores, (iv) were salt and pH-tolerant, and (v) were not known pathogens. These traits (and others) strongly suggest plant growth promotion ability [6,7].…”

Section: Introductionmentioning

confidence: 68%

“…The objective of this research was to assess microbial diversity in an agricultural soil in Botswana to identify rhizobial strains and PGPB, which are adapted to this ecosystem. To do this, we performed both 1) cultivation-independent analysis of the soil and also of legume root nodules to determine the differences in microbial diversity in addition to 2) cultivation-dependent analyses (i) of rhizospheres collected in two different years and (ii) isolations of plant-selected bacteria from the interior of root nodules of different legumes used as trap plants [6,7]. Because only 1-3% of bacteria in soil can be cultured, we reasoned that the nodule-isolated bacteria that could grow on artificial media might serve as potential inoculants because they were selected by the legume host.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of potential plant growth-promoting bacteria from nodules of legumes grown in arid Botswana soil

Kosty¹,

Pule‐Meulenberg

Humm³

et al. 2020

Preprint

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As the world population increases, improvements in crop growth and yield will be needed to meet rising food demands, especially in countries that have not developed agricultural practices optimized for their own soils and crops. In many African countries, farmers improve agricultural productivity by applying synthetic fertilizers and pesticides to crops, but their continued use over the years has had serious environmental consequences including air and water pollution as well as loss of soil fertility. To reduce the overuse of synthetic amendments, we are developing inocula for crops that are based on indigenous soil microbes, especially those that enhance plant growth and improve agricultural productivity in a sustainable manner. We first isolated environmental DNA from soil samples collected from an agricultural region to study the composition of the soil microbiomes and then used Vigna unguiculata (cowpea), an important legume crop in Botswana and other legumes as “trap” plants using the collected soil to induce nitrogen-fixing nodule formation. We have identified drought-tolerant bacteria from Botswana soils that stimulate plant growth; many are species of Bacillus and Paenibacillus. In contrast, the cowpea nodule microbiomes from plants grown in these soils house mainly rhizobia particularly Bradyrhizobium, but also Methylobacterium spp. Hence, the nodule microbiome is much more limited in non-rhizobial diversity compared to the soil microbiome, but also contains a number of potential pathogenic bacteria.

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Section: Introductionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Isolation of potential plant growth-promoting bacteria from nodules of legumes grown in arid Botswana soil

Kosty¹,

Pule‐Meulenberg

Humm³

et al. 2020

Preprint

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“…Alternatively, it is possible that communication between rhizobia and other bacteria in the soil (i.e., quorum sensing) may underlie the increase in nodulation (Miao et al, 2018). It has also recently become clear that rhizobia are not the only bacteria living in nodules (Martínez‐Hidalgo & Hirsch, 2017; Tapia‐García et al, 2020). If these co‐habiting bacteria facilitate nodulation, it could account for the effect of the microbiome on nodulation we observed, especially if plant genotypes vary in their recruitment of these nodule‐associated bacteria.…”

Section: Discussionmentioning

confidence: 99%

The soil microbiome increases plant survival and modifies interactions with root endosymbionts in the field

Markalanda

McFadden

Cassidy

et al. 2022

Ecology and Evolution

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Evidence is accumulating that the soil microbiome—the community of microorganisms living in soils—has a major effect on plant traits and fitness. However, most work to date has taken place under controlled laboratory conditions and has not experimentally disentangled the effect of the soil microbiome on plant performance from the effects of key endosymbiotic constituents. As a result, it is difficult to extrapolate from existing data to understand the role of the soil microbiome in natural plant populations. To address this gap, we performed a field experiment using the black medick Medicago lupulina to test how the soil microbiome influences plant performance and colonization by two root endosymbionts (the mutualistic nitrogen‐fixing bacteria Ensifer spp. and the parasitic root‐knot nematode Meloidogyne hapla) under natural conditions. We inoculated all plants with nitrogen‐fixing bacteria and factorially manipulated the soil microbiome and nematode infection. We found that plants grown in microbe‐depleted soil exhibit greater mortality, but that among the survivors, there was no effect of the soil microbiome on plant performance (shoot biomass, root biomass, or shoot‐to‐root ratio). The soil microbiome also impacted parasitic nematode infection and affected colonization by mutualistic nitrogen‐fixing bacteria in a plant genotype‐dependent manner, increasing colonization in some plant genotypes and decreasing it in others. Our results demonstrate the soil microbiome has complex effects on plant–endosymbiont interactions and may be critical for survival under natural conditions.

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“…The results of this study also showed that several bacterial genera were significantly more abundant in the rhizosphere of healthy trees than in dead trees. Among these bacterial genera, as evidenced in previous studies, several species belonging to these groups are known as plant-growth-promoting and beneficial rhizosphere microbes, including root nodulators and free-living nitrogen-fixing bacteria (e.g., Bradyrhizobium, Rhizomicrobium, Caulobacter, Nitrosospira, Rhizobacter, Paraburkholderia, Rhizobium, Devosia, Caballeronia, Niveispirillum, Dyella, and Herbaspirillum ), beneficial Actinomycetes (e.g., Frankia, Streptomyces, and Actinoallomurus ), and other beneficial metabolite-, phytohormone-, and siderophore-producing phytopathogen-antagonistic bacteria (e.g., Lysobacter, Luteibacter, Mucilaginibacter, and Variovorax ) [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Dysbiosis in the Rhizosphere Microbiome of Standing Dead Korean Fir (Abies koreana)

Han

Mannaa

Jeon

et al. 2022

Plants

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The Korean fir (Abies koreana), a native coniferous tree species mainly found on Mt. Halla in Jeju, South Korea, is suffering from continuous population decline and has been declared an endangered species. Research efforts have focused on the possible abiotic causes behind this worrying decline. However, the potential link between tree vitality and the rhizosphere microbiome remains unclear. In this study, a comparative metagenomic 16S rRNA sequence analysis was used to investigate the composition of the rhizosphere microbiota of samples collected from healthy and die-back-affected trees on Mt. Halla. The results indicated a significant reduction in the richness and diversity of microbiota in the rhizosphere of die-back-affected trees. Moreover, the relative abundance of Proteobacteria, Actinobacteria, and Bacteroidetes were significantly higher in healthy trees than in standing dead trees. Many bacterial genera were significantly more abundant in the rhizosphere of healthy trees, including those known for promoting plant growth and tolerance to biotic and abiotic stresses (e.g., Bradyrhizobium, Rhizomicrobium, Caulobacter, Nitrosospira, Rhizobacter, Paraburkholderia, Rhizobium, Devosia, Caballeronia, Niveispirillum, Dyella, Herbaspirillum, Frankia, Streptomyces, Actinoallomurus, Lysobacter, Luteibacter, Mucilaginibacter, and Variovorax). To our knowledge, this is the first report on rhizosphere bacterial microbiome dysbiosis in die-back-affected Korean fir trees, suggesting that the influence of rhizosphere microbiota should be considered to save this endangered species by investigating possible intervention strategies in future work.

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Plant growth-promoting bacteria isolated from wild legume nodules and nodules of Phaseolus vulgaris L. trap plants in central and southern Mexico

Cited by 43 publications

References 71 publications

Isolation of potential plant growth-promoting bacteria from nodules of legumes grown in arid Botswana soil

Isolation of potential plant growth-promoting bacteria from nodules of legumes grown in arid Botswana soil

The soil microbiome increases plant survival and modifies interactions with root endosymbionts in the field

Dysbiosis in the Rhizosphere Microbiome of Standing Dead Korean Fir (Abies koreana)

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