Plant growth-promoting bacteria (PGPBs) and copper (II) oxide (CuO) nanoparticle ameliorates DNA damage and DNA Methylation in wheat (Triticum aestivum L.) exposed to NaCl stress

Türkoğlu, Aras; İlhan, Emre; Özkan, Güller; Öztürk, Halil İbrahim; Haliloğlu, Kamil; Cinisli, Kağan Tolga

doi:10.1007/s13562-021-00713-w

Cited by 24 publications

(23 citation statements)

References 48 publications

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“…This is probably due to the frequent use of MSH in callus and cell cultures, which induces genetic abnormalities such as polyploidy and DNA endo-replication. These findings are similar to those reported in the literature, from studies that determined epigenetic modifications in different plants under abiotic stress conditions [ 35 , 36 , 37 , 50 , 51 ].…”

Section: Discussionsupporting

confidence: 92%

“…Means were calculated for each experimental group, and changes in the means of each group were calculated as a percentage compared to the control. Mean polymorphism values for the CRED-iPBS analysis were calculated using the formula 100 × a/n [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

“…The CRED-iPBS technique also has been successfully applied in many studies of different plant species to determine their methylation patterns. The CRED-iPBS technique is used to detect methylation changes between different tissues or different stages of development after restriction digestion of DNA with methylation sensitive enzymes such as HpaII and MspI [ 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)

Türkoğlu

Haliloğlu

Balpınar

et al. 2022

Plants

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Mammalian sex hormones are steroid-structured compounds that support the growth and development of plants at low concentrations. Since they affect the physiological processes in plants, it has been thought that mammalian sex hormones may cause modifications to plant genomes and epigenetics. This study aims to determine whether different mammalian sex hormones (17 β-estradiol, estrogen, progesterone, and testosterone) in several concentrations (0, 10−4, 10−6, and 10−8 mM) affect genetic or epigenetic levels in bean plants, using in vitro tissue cultures from plumule explants. We investigated levels of DNA damage, changes in DNA methylation and DNA stability in common bean exposed to mammalian sex hormones (MSH) using inter-primer binding site (iPBS) and Coupled Restriction Enzyme Digestion-iPBS (CRED-iPBS) assays, respectively. The highest rate of polymorphism in iPBS profiles was observed when 10−4 mM of estrogen (52.2%) hormone was administered. This finding indicates that genetic stability is reduced. In the CRED-iPBS profile, which reveals the methylation level associated with the DNA cytosine nucleotide, 10−4 mM of estrogen hormone exhibited the highest hypermethylation value. Polymorphism was observed in all hormone administrations compared to the control (without hormone), and it was determined that genomic stability was decreased at high concentrations. Taken together, the results indicate that 17 β-estradiol, estrogen, progesterone, and testosterone in bean plants affect genomic instability and cause epigenetic modifications, which is an important control mechanism in gene expression.

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Section: Discussionsupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)

Türkoğlu

Haliloğlu

Balpınar

et al. 2022

Plants

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“…) technique involves DNA pro ling with the use of molecular markers. It is used to identify changes in cytosine methylation stress-induced toxicity in plant genomes (Hosseinpour et al 2021;Hosseinpour et al 2019b). This study was conducted to investigate the effects of MSH on REC induction and regeneration of wheat plants.…”

Section: Cred (Coupled Restriction Enzyme Digestionmentioning

confidence: 99%

“…However, DNA analysis with molecular marker technology offers greater sensitivity and selectivity. Molecular markers have successfully been used to determine polymorphism and DNA metalations and iPBS was used to elucidate the effect of different treatments on plants (Hosseinpour et al 2021). The iPBS offers a universal method for DNA ngerprinting (Kalendar et al (2010).…”

Section: Introductionmentioning

confidence: 99%

Effects of mammalian sex hormones on regeneration capacity, retrotransposon polymorphism and genomic instability in wheat (Triticum aestivum L.)

Türkoğlu

2022

Plant Cell Tiss Organ Cult

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Mammalian steroid hormones (MSH) are also inherently synthesized by several plant species. However, external application of steroid hormones further stimulates cell division, pollen germination, plant growth and development. There is little known about the effects of MSH on polymorphism and DNA damage in Triticum aestivum L under in vitro conditions. In this study, inter-primer binding site (iPBS) retrotransposon and coupled restriction enzyme digestion-iPBS (CRED-iPBS) markers were used for detection of the variation in responded embryogenic callus (REC) that were obtained from endosperm-supported mature embryo of wheat in Murashige and Skoog (MS) medium containing different concentrations [0 (control), 10 -4 , 10 -6 and 10 -8 m mol L −1 ] of MSH (progesterone, 17β-estradiol, estrone and testosterone). Responded embryogenic callus (REC) and regenerable callus (RE) induction from mature embryos varied with MSH type and concentrations. The highest level of genomic template stability (GTS) value (80.52%) was obtained from 10 -8 mM progesterone treatments and the lowest value (68.83%) from 10 -4 mM 17 β-estradiol treatments. Epigenetic changes were more frequent and variable than the genetic changes. While DNA hypermethylation was observed at higher 17 β-estradiol concentrations, DNA hypomethylation was observed in progesterone treatments. It was concluded based on iPBS and CRED-iPBS ndings that high MSH concentrations caused DNA changes and methylation.

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Potential Application of Nanotechnology in Biofertilizer Formulation for Millets

Rana,

Pudake

2023

Rhizosphere Biology

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Plant growth-promoting bacteria (PGPBs) and copper (II) oxide (CuO) nanoparticle ameliorates DNA damage and DNA Methylation in wheat (Triticum aestivum L.) exposed to NaCl stress

Cited by 24 publications

References 48 publications

The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)

The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)

Effects of mammalian sex hormones on regeneration capacity, retrotransposon polymorphism and genomic instability in wheat (Triticum aestivum L.)

Potential Application of Nanotechnology in Biofertilizer Formulation for Millets

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