Plant-Made Vaccines in the Fight Against Cancer

Wong-Arce, Alejandra; González‐Ortega, Omar; Rosales‐Mendoza, Sergio

doi:10.1016/j.tibtech.2016.12.002

Cited by 23 publications

(10 citation statements)

References 85 publications

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“…Natural products are important sources of existing drugs and play a significant role in the discovery of antitumor drugs 8,9. In terms of antineoplastic drugs alone, about two-thirds come from natural products 10.…”

Section: Introductionmentioning

confidence: 99%

<p>Sanguinarine exhibits potent efficacy against cervical cancer cells through inhibiting the STAT3 pathway in vitro and in vivo</p>

Zhang¹,

Zhang²,

Venkat³

et al. 2019

CMAR

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Background Cervical cancer is the third most common malignancy among female cancer patients worldwide. Signal transducer and activator of transcription 3 (STAT3) is a transcription factor which regulates a variety of cancer cellular physiological activities including cervical cancer. Sanguinarine (SNG) is a natural plant-derived benzophenanthridine alkaloid that possesses antitumor activities in several cancer cells. However, its anticancer effect on human cervical cancer cells and the underlying mechanisms have not been fully defined. Methods In this study, the inhibitory effect of SNG on the proliferation and growth of HeLa cell was detected by MTT assay. Next, cell cycle and apoptosis of HeLa cells was analyzed using Annexin-V/PI double staining and flow cytometry. Then, we measured intracellular ROS generation induced by SNG in HeLa cells by DCFH-DA (10 μM) staining, and the expression level of p-STAT3 and STAT3 was detected by Western blot. Finally, in order to study the effect of SNG on tumor growth in vivo, athymic nude mice were used in the vivo experiments. Result This study showed that SNG dose-dependently decreased the tumor cell proliferation and induced a marked increase in cell apoptosis in HeLa cells. Western blot analysis results revealed that SNG-induced antitumor effect might be mediated by STAT3 inhibition. SNG increased the expression of the proapoptotic protein Bax and reduced the expression of the antiapoptotic protein Bcl-2. We further found that SNG dose-dependently increased ROS level in Hela cells. Moreover, pretreatment with N-acetyl-l-cysteine, a scavenger of ROS, almost reversed the SNG-induced anticancer effect. In addition, SNG inhibited human cervical cancer xenograft growth without exhibiting toxicity in vivo. Conclusion Our findings highlight STAT3 as a promising therapeutic target. We also demonstrate that SNG is a novel anticancer drug for the treatment of cervical cancer.

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Section: Introductionmentioning

confidence: 99%

<p>Sanguinarine exhibits potent efficacy against cervical cancer cells through inhibiting the STAT3 pathway in vitro and in vivo</p>

Zhang¹,

Zhang²,

Venkat³

et al. 2019

CMAR

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“…Immunogenicity induced by plant-specific glycans is one of the main objections against plant-based production of biotherapeutics. Contrary to this argument, experimental evidence showed that the plant-specific glycans do not interfere with the immune system and at times it is rather beneficial [56,136,137]. The N-glycosylation pattern of plant glycoproteins is different from the animals.…”

Section: Glycoengineering For the Mammalian Type Of Glycosylationmentioning

confidence: 93%

Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update

Satheeshkumar

2020

Mol Biotechnol

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Single chain variable fragments (scFvs) are generated by joining together the variable heavy and light chain of a monoclonal antibody (mAb) via a peptide linker. They offer some advantages over the parental mAb such as low molecular weight, heterologous production, multimeric form, and multivalency. The scFvs were produced against more than 50 antigens till date using 10 different plant species as the expression system. There were considerable improvements in the expression and purification strategies of scFv in the last 24 years. With the growing demand of scFv in therapeutic and diagnostic fields, its biosynthesis needs to be increased. The easiness in development, maintenance, and multiplication of transgenic plants make them an attractive expression platform for scFv production. The review intends to provide comprehensive information about the use of plant expression system to produce scFv. The developments, advantages, pitfalls, and possible prospects of improvement for the exploitation of plants in the industrial level are discussed.

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“…The technology has rapidly developed, and the potential drawbacks associated with plant molecular farming during their early stages of development, including the need for a high protein expression level and efficient downstream processing, have now been achieved. The advantages of plant expression platforms are cited in several earlier reports showing head-to-head comparisons with other existing platforms (Table 1) [10][11][12][13][14][15][16][17][18]. The key advantages of all plant-based systems are easy cultivation, low expenses, low or no pathogen load, rapid mass production of recombinant proteins, and the ability of the plants to assemble complex proteins with eukaryotic-like post-translational modifications (PTMs) [19].…”

Section: Plant Expression Platformmentioning

confidence: 99%

Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical Production

Shanmugaraj

Bulaon

Phoolcharoen

2020

Plants

172

166

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The demand for recombinant proteins in terms of quality, quantity, and diversity is increasing steadily, which is attracting global attention for the development of new recombinant protein production technologies and the engineering of conventional established expression systems based on bacteria or mammalian cell cultures. Since the advancements of plant genetic engineering in the 1980s, plants have been used for the production of economically valuable, biologically active non-native proteins or biopharmaceuticals, the concept termed as plant molecular farming (PMF). PMF is considered as a cost-effective technology that has grown and advanced tremendously over the past two decades. The development and improvement of the transient expression system has significantly reduced the protein production timeline and greatly improved the protein yield in plants. The major factors that drive the plant-based platform towards potential competitors for the conventional expression system are cost-effectiveness, scalability, flexibility, versatility, and robustness of the system. Many biopharmaceuticals including recombinant vaccine antigens, monoclonal antibodies, and other commercially viable proteins are produced in plants, some of which are in the pre-clinical and clinical pipeline. In this review, we consider the importance of a plant- based production system for recombinant protein production, and its potential to produce biopharmaceuticals is discussed.

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Plant-Made Vaccines in the Fight Against Cancer

Cited by 23 publications

References 85 publications

<p>Sanguinarine exhibits potent efficacy against cervical cancer cells through inhibiting the STAT3 pathway in vitro and in vivo</p>

<p>Sanguinarine exhibits potent efficacy against cervical cancer cells through inhibiting the STAT3 pathway in vitro and in vivo</p>

Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update

Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical Production

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