Plant metabolic activation of 1,2-dibromoethane (EDB) to a mutagen of greater potency

Scott, Barry R.; Sparrow, A.H.; Schwemmer, Susan S.; Schairer, L.A.

doi:10.1016/0027-5107(78)90158-6

Cited by 59 publications

(12 citation statements)

References 20 publications

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“…It has been shown that EDB can be converted into a mutagen via two reaction pathways, one involving a breakdown product in vitro and the other involving metabolic activation by plants, the latter being apparently energydependent as that for mammalian microsomal activation of EDB (Scott et al, 1978), although the mechanism of the activation of EDB in plant cells has not yet been fully understood. The incomplete results but showing an apparent tendency of antagonistic effect observed in the present study by exposing to X rays 20 h after the 4-h EDB treatment may suggest that the activation of EDB might be inhibited by X rays delivered during the activation period of EDB, as in cases of other promutagens, MH Ichikawa, 1995, 1996), PDA and DMN (Xiao and Ichikawa, 1998).…”

Section: Resultsmentioning

confidence: 99%

Mutagenic synergism detected between 1,2-dibromoethane and X rays in the stamen hairs of Tradescantia clone BNL 4430.

Xiao

Ichikawa

1998

Genes Genet. Syst.

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Mutagenic interaction between 1,2-dibromoethane (EDB) and X rays was studied in the stamen hairs of Tradescantia clone BNL 4430, a blue/pink heterozygote. The young inflorescence-bearing shoots with roots of this clone cultivated in a nutrient solution circulating growth chamber were used as the tester plants. EDB is a promutagen and also a bifunctional alkylating agent with a high Swain-Scott substrate constant, but is thought to react probably via SN 1 mechanism. After confirming the dose-dependent mutagenicities of aqueous solutions of EDB for the first time in Tradescantia stamen hairs, a combined treatment with EDB and X rays was conducted, exposing acutely to 578 mGy X rays at the midpoint of 66.5 mM EDB treatment for 4 h. The induced somatic mutation frequency determined after the combined treatment was significantly higher (at 0.1% level) than that expected from the additive effects of EDB and X rays, showing that EDB and X rays acted obviously synergistically. The confirmation of the mutagenic synergism between EDB and X rays is reported here for the first time, although a likelihood of synergistic effects of EDB with 3 H beta rays has been suggested earlier.

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Section: Resultsmentioning

confidence: 99%

Mutagenic synergism detected between 1,2-dibromoethane and X rays in the stamen hairs of Tradescantia clone BNL 4430.

Xiao

Ichikawa

1998

Genes Genet. Syst.

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“…Scott et al [1977] reported that ethylene dibromide was metabolized by Tradescuntiu homogenate into a mutagen of greater potency. Additionally, ethanol was activated by Viciu fubu root S-10 [ Takehisa and Kanaya, 19831 and atrazine was activated by potato S-9 [Loprieno and Adler, 19801, but neither chemical was activated by mammalian hepatic S-9.…”

Section: Discussionmentioning

confidence: 99%

In vitro enhancement of the mutagenicity of 4‐nitro‐o‐phenylenediamine by plant S‐9

Gentile

Townsend

et al. 1985

Environ. mutagen

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The direct-acting mutagen 4-nitro-o-phenylenediamine (NOP) was activated in vitro by pea or tobacco S-9 into a more potent mutagen in Salmonella typhimurium strain TA98. NOP mutagenicity was not altered by Aroclor 1254-induced rat liver S-9. The plant S-9 activation of NOP was heat-sensitive but was not NADPH-dependent, did not involve superoxide radicals, and was not inhibited by CO. A direct relationship between plant peroxidase and NOP activation was established. Several purified peroxidases including horseradish peroxidase, chloroperoxidase, and lactoperoxidase also activated NOP. The perodoxidative process was not H2O2-dependent but was partially inhibited by a peroxidase inhibitor.

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“…The haloalkane 1,2-dibromoethane (EDB) widely used as a pesticide, fumigant, and gasoline additive, was plant activated into a mutagen of greater potency (48). crude homogenates from plants of Tradescantia clone 4430 were prepared and, coincubated with EDB and E. coli or A. nidulans.…”

Section: In Vitro Plant Activationmentioning

confidence: 99%

Plant Dependent Mutation Assays

Gentile

Plewa

1982

Genetic Toxicology

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Plant metabolic activation of 1,2-dibromoethane (EDB) to a mutagen of greater potency

Cited by 59 publications

References 20 publications

Mutagenic synergism detected between 1,2-dibromoethane and X rays in the stamen hairs of Tradescantia clone BNL 4430.

Mutagenic synergism detected between 1,2-dibromoethane and X rays in the stamen hairs of Tradescantia clone BNL 4430.

In vitro enhancement of the mutagenicity of 4‐nitro‐o‐phenylenediamine by plant S‐9

Plant Dependent Mutation Assays

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