Plant Paralog to Viral Movement Protein That Potentiates Transport of mRNA into the Phloem

Xoconostle‐Cázares, Beatriz; Yu, Xiang; Ruíz-Medrano, Roberto; Wang, Hongli; Monzer, Jan; Yoo, Byung-Chun; McFarland, Keith; Franceschi, Vincent R.; Lucas, William J.

doi:10.1126/science.283.5398.94

Cited by 431 publications

(336 citation statements)

References 26 publications

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“…A chimeric virus, in which the TMV MP gene was replaced by the RCNMV MP gene, systemically infected both N. benthamiana and tobacco, although tobacco is not a systemic host of RCNMV (14). Evidence for a paralog of the RCNMV 35-kDa MP in Cucurbita maxima has been found (33). This protein has been shown to move RNA within the phloem, the long-distance transport system that delivers nutrients and hormones to plant tissues and organs.…”

Section: Discussionmentioning

confidence: 99%

Systemic spread of an RNA insect virus in plants expressing plant viral movement protein genes

Dasgupta

Garcia

Goodman

2001

Proc. Natl. Acad. Sci. U.S.A.

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nodavirus V iruses whose host range spans two kingdoms are few. Most are insect-vectored plant viruses that multiply in a narrow insect host range (1). Flock house virus (FHV), a member of the insect͞animal virus family called Nodaviridae (for reviews, see refs. 2-4), is a unique example of a virus that crosses the kingdom barrier. FHV was originally isolated from the New Zealand grass grub Costelytra zealandica (5). The range of insect hosts for FHV is not well studied. In the laboratory, FHV replicates in the larvae of the wax moth Galleria mellonella and cultured Drosophila melanogaster cells (4). In 1990, FHV was shown to replicate and produce infectious virions in barley protoplasts and the inoculated leaves of several other monocotyledon and dicotyledon plant species without producing symptoms (6). FHV thus replicates in both insects (2) and plants (6), although it is not transmitted to plants by its insect hosts (2).The genome of FHV consists of two small messenger sense RNAs: RNA1 (3.1 kb; ref. Broad host range, simple genome organization, and asymptomatic growth in plants make FHV a powerful tool for the study of fundamental questions of virus-host interactions and the modification of gene expression in plants. A drawback to its use is that FHV does not encode a protein that enables it to move inside the plant. In all plants previously tested, FHV accumulated only in inoculated tissues or cells (6).Here we report the results of experiments designed to determine whether FHV could be mobilized in plants with the use of plant viral movement proteins (MPs). We used genes encoding the 30-kDa protein of tobacco mosaic virus (TMV) and the 35-kDa protein of red clover necrotic mosaic virus (RCNMV) (dianthovirus), two of the most well-studied and widely used plant viral MPs, both expressed as transgenes in N. benthamiana plants (11-16). These plant viruses each encode a single, functionally homologous MP with conserved amino acid sequences (14, 17), and neither virus requires coat protein for cell-to-cell movement (16, 18). These MPs have been shown conclusively to bind RNA in vitro and to increase the size exclusion limit of plasmodesmata in mesophyl cells to allow cell-to-cell movement (19). Moreover, both TMV MP and RCNMV MP have been shown to complement the transport of other plant viruses belonging to different taxonomic groups, such as barley stripe mosaic virus (20, 21), potato virus X (22), and brome mosaic virus (23).Our results show that MPs of both TMV and RCNMV initiate local as well as long-distance movement of FHV in MP transgenic plants. RCNMV MP is more effective in mobilizing movement of FHV to noninoculated leaves. We also show that FHV replicates in inoculated leaves of six more plant species, in addition to those reported previously (6). Newly discovered experimental hosts of FHV include alfalfa, Arabidopsis, Brassica, cucumber, rice, and sweet corn. Plants were grown from seeds planted in Scotts Redi-earth Plug and Seedling Mix and placed in growth chambers maintained at 24°C, with 12 h of l...

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Section: Discussionmentioning

confidence: 99%

Systemic spread of an RNA insect virus in plants expressing plant viral movement protein genes

Dasgupta

Garcia

Goodman

2001

Proc. Natl. Acad. Sci. U.S.A.

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“…1 The identification of the plant functional analogs of viral MPs suggests the presence of endogenous RNA transport machinery in transportation of non-cell autonomous RNAs. 37,38 Previously, the movement of KN1 RNA was found to be mediated through its own protein. 39,40 However, when FT protein was retained in companion cells, FT RNA still moved long distance to the scion (Fig.…”

Section: P35s-ft-ir/p35s-rfp-ft P35s-ft-ir/p35s-gfp-ftmentioning

confidence: 99%

Long-distance movement of ArabidopsisFLOWERING LOCUS TRNA participates in systemic floral regulation

Huang

Liu

et al. 2012

RNA Biology

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“…This technique has proven to yield highresolution of specific mRNA amplification signals on several plant tissues (Ruiz-Medrano et al, 1999;Xoconostle-Cázares et al, 1999).…”

Section: Sps1 Expression In Leavesmentioning

confidence: 99%

Tissue-Specific and Developmental Pattern of Expression of the Rice sps1 Gene

et al. 2000

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Sucrose-phosphate synthase (SPS) is one of the key regulatory enzymes in carbon assimilation and partitioning in plants. SPS plays a central role in the production of sucrose in photosynthetic cells and in the conversion of starch or fatty acids into sucrose in germinating seeds. To explore the mechanisms that regulate the tissue-specific and developmental distribution of SPS, the expression pattern of rice (Oryza sativa) sps1 (GenBank accession no. U33175) was examined by in situ reverse transcriptase-polymerase chain reaction and the expression directed by the sps1 promoter using the ␤-glucuronidase reporter gene. It was found that the expression of the rice sps1 gene is limited to mesophyll cells in leaves, the scutellum of germinating seedlings, and pollen of immature inflorescences. During leaf development, the sps1 promoter directs a basipetal pattern of expression that coincides with the distribution of SPS activity during the leaf sink-to-source transition. It was also found that during the vegetative part of the growth cycle, SPS expression and enzymatic activity are highest in the youngest fully expanded leaf. Additionally, it was observed that the expression of the sps1 promoter is regulated by light and dependent on plastid development in photosynthetic tissues, whereas expression in scutellum is independent of both light and plastid development.

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Plant Paralog to Viral Movement Protein That Potentiates Transport of mRNA into the Phloem

Cited by 431 publications

References 26 publications

Systemic spread of an RNA insect virus in plants expressing plant viral movement protein genes

Systemic spread of an RNA insect virus in plants expressing plant viral movement protein genes

Long-distance movement of ArabidopsisFLOWERING LOCUS TRNA participates in systemic floral regulation

Tissue-Specific and Developmental Pattern of Expression of the Rice sps1 Gene

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