Plant-Parasitic Nematodes Attacking Cotton in the United States: Old and Emerging Production Challenges

Koenning, S. R.; Wrather, J. A.; Kirkpatrick, T. L.; Walker, Nathan; Starr, J. L.; Mueller, John

doi:10.1094/pdis.2004.88.2.100

Cited by 100 publications

(83 citation statements)

References 38 publications

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“…a Shoot weight, b leaf/stem weight ratio, and c plant height were used as parameters to score diseaseseverity, 3 weeks following stem inoculation with the pathogen. Data represent mean ± SE, * P \ 0.05; ** P \ 0.001, n = 10 to the nematodes (Robinson et al 1999;Koenning et al 2004;Blasingame et al 2008). Thus, there is an urgent need for measures to control losses caused by these parasites.…”

Section: Discussionmentioning

confidence: 99%

“…8a). Cotton plants growing in nematode infested soil under field conditions, suffer from nutritional deficiencies, fruit abortion, and abnormal maturation of the crop (Koenning et al 2004). Even under controlled environmental conditions in a 68L-19 demonstrated a stronger resistance to reniform nematodes compared to the line 68L-20 that showed moderate level resistance.…”

Section: Atnpr1-mediated Resistance To Fungal Pathogens In Transgenicmentioning

confidence: 99%

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Resistance against various fungal pathogens and reniform nematode in transgenic cotton plants expressing Arabidopsis NPR1

et al. 2010

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Cotton is an economically important crop worldwide that suffers severe losses due to a wide range of fungal/bacterial pathogens and nematodes. Given its susceptibility to various pathogens, it is important to obtain a broad-spectrum resistance in cotton. Resistance to several fungal and bacterial diseases has been obtained by overexpressing the Non-expressor of Pathogenesis-Related genes-1 (NPR1) in various plant species with apparently minimal or no pleiotropic effects. We examined the efficacy of this approach in cotton by constitutive expression of the Arabidopsis (Arabidopsis thaliana) NPR1 gene. The results show that NPR1-expressing lines exhibited significant resistance to Verticillium dahliae isolate TS2, Fusarium oxysporum f. sp. vasinfectum, Rhizoctonia solani, and Alternaria alternata. Interestingly, the transformants also showed significant resistance to reniform nematodes. Analysis of defense-related, biochemical and molecular responses suggest that when challenged with pathogens or certain systemic acquired resistance-inducing chemicals, the transgenic lines respond to a greater degree compared to the wild-type plants. Importantly, the basal activities of the defense-related genes and enzymes in uninduced transformants were no different than those in their non-transgenic counterparts. The results provide additional evidence supporting the role of NPR1 as an important part of the plant defense system and suggest a means to achieve broad-spectrum resistance to pathogens via genetic engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Atnpr1-mediated Resistance To Fungal Pathogens In Transgenicmentioning

confidence: 99%

Resistance against various fungal pathogens and reniform nematode in transgenic cotton plants expressing Arabidopsis NPR1

et al. 2010

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“…The southern root-knot nematode (RKN) [Meloidogyne incognita (Kofoid and White) Chitwood] is a major pest across U. S. cotton production areas (Koenning et al 2004). Cotton, Gossypium hirsutum L., germplasm lines with genes for RKN resistance have been released, but no cultivar on the market today has a high level of resistance, although cultivars with moderate levels of resistance are available.…”

Section: Introductionmentioning

confidence: 99%

SSR markers for marker assisted selection of root-knot nematode (Meloidogyne incognita) resistant plants in cotton (Gossypium hirsutum L)

et al. 2011

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Cotton (Gossypium hirsutum L) cultivars highly resistant to the southern root-knot nematode (RKN) [Meloidogyne incognita (Kofoid and White) Chitwood] are not available. Resistant germplasm lines are available; however, the difficulty of selecting true breeding lines has hindered applied breeding and no highly resistant cultivars are available to growers. Recently, molecular markers on chromosomes 11 and 14 have been associated with RKN resistance, thus opening the way for marker assisted selection (MAS) in applied breeding. Our study aimed to determine the utility of these markers for MAS. Cross one was RKN resistant germplasm M240 RNR 9 the susceptible cultivar, FM966 and is representative of the initial cross a breeder would make to develop a RKN resistant cultivar. Cross two consists of Clevewilt 6 9 Mexico Wild (PI563649), which are the two lines originally used to develop the first highly RKN resistant germplasm. Mexico Wild is photoperiodic. We phenotyped the F 2 of cross one for gall index and number of RKN eggs per plant and genotyped each plant for CIR 316 (chromosome 11) and BNL 3661 (chromosome 14). From this, we verified that MAS was effective, and the QTL on chromosome 14 was primarily associated with a dominant RKN resistance gene affecting reproduction. In the first F 2 population of cross two, we used MAS to identify 11 plants homozygous for the markers on chromosomes 11 and 14, and which also flowered in long days. Progeny of these 11 plants were phenotyped for RKN gall index and egg number and confirmed as RKN highly resistant plants. Generally about 7-10 generations of RKN phenotyping and progeny testing were required to develop the original RKN highly resistant germplasms. Our results show that commercial breeders should be able to use the markers in MAS to rapidly develop RKN resistant cultivars. Keywords Cotton breeding Á Genetics Á Marker assisted breeding Á Plant breeding Á Root-knot nematode Abbreviations RKN Root-knot nematode MAS Marker assisted selection FM966 Fibermax cultivar SSR Simple sequence repeat BNL

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“…columbus, H. galeatus and H. magnistylus, are found widely in the south-eastern United States. They are considered to be economically important nematode pests and can cause serious damage to agronomic crops, including cotton (Gossypium hirsutum L.), corn (Zea mays L.) and soybean (Glycine max L.) (Fassuliotis, 1974;Nyczepir & Lewis, 1979;Robbins et al, 1987Robbins et al, , 1989Henn & Dunn, 1989;Noe, 1993;Koenning et al, 2004). Hoplolaimus columbus, which is endo-and ectoparasitic on cotton and soybean, causes severe damage to cortical parenchyma and endodermal-vascular regions of both plants (Lewis et al, 1976).…”

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confidence: 99%

Molecular identification of some Hoplolaimus species from the USA based on duplex PCR, multiplex PCR and PCR-RFLP analysis

Bae

Robbins

Szalanski

2009

Nematol

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Two different molecular approaches, a multiplex PCR and PCR-RFLP of ITS-rDNA, were developed for the identification of Hoplolaimus species. DNA sequences of H. columbus, H. galeatus, H. concaudajuvencus, H. magnistylus, H. seinhorsti and three undescribed Hoplolaimus species were used to design species-specific primers. Three reverse species-specific PCR primers for H. columbus, H. galeatus and H. magnistylus were developed using the ITS1 region exhibiting interspecific variation. Three speciesspecific PCR primers in combination with the forward primer, Hoc-1f, produced distinct amplicons of 580 bp for H. columbus, 120 bp for H. galeatus and 340 bp for H. magnistylus. We successfully identified each of three species by multiplex PCR when all three were mixed in a single PCR reaction. Restriction enzyme digests of the PCR amplicon using HaeIII and RsaI permitted discrimination of H. columbus, H. galeatus, H. magnistylus, H. concaudajuvencus, H. sp. 1, H. sp. 2 and H. sp. 3 from each other. These results suggest that these molecular techniques allow for rapid, easy and reliable identification of Hoplolaimus species.

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Plant-Parasitic Nematodes Attacking Cotton in the United States: Old and Emerging Production Challenges

Cited by 100 publications

References 38 publications

Resistance against various fungal pathogens and reniform nematode in transgenic cotton plants expressing Arabidopsis NPR1

Resistance against various fungal pathogens and reniform nematode in transgenic cotton plants expressing Arabidopsis NPR1

SSR markers for marker assisted selection of root-knot nematode (Meloidogyne incognita) resistant plants in cotton (Gossypium hirsutum L)

Molecular identification of some Hoplolaimus species from the USA based on duplex PCR, multiplex PCR and PCR-RFLP analysis

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