Plant-PET Scans: In Vivo Mapping of Xylem and Phloem Functioning

Hubeau, Michiel; Steppe, Kathy

doi:10.1016/j.tplants.2015.07.008

Cited by 80 publications

(81 citation statements)

References 84 publications

(162 reference statements)

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“…This technique was originally pioneered by Minchin in the mid-1970's and has continued to be used in subsequent years (Minchin & Thorpe, 2003). Although somewhat neglected in the past decade, radioactive-tracking techniques have undergone something of a renaissance due to the advent of positron emission tomography (PET) techniques being used in plant research (Hubeau & Steppe, 2015). One drawback of CO2-based techniques is that they require CO2 uptake, a process which does not necessarily cover the full 24 hour period in many plant species.…”

Section: Phloem Transportmentioning

confidence: 99%

“…Phloem transport is one of the major determinants of plant growth and therefore its thorough understanding may help in optimising plant growth conditions in order to increase crop yield. However, our current understanding of phloem transport is far from complete (Thompson, 2006;Hubeau & Steppe, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Although hypotheses about xylem and phloem transport mechanisms exist for almost a century (i.e., cohesion tension theory and Munch pressure flow), the measurement of both, but particularly phloem transport, has remained experimentally elusive (Minchin & Thorpe, 2003;De Schepper et al, 2013;Hubeau & Steppe, 2015). The major drawback of most reported techniques lies in their invasiveness, which can precipitate an immediate defence reaction in the phloem transport pathway or may lead to embolism within xylem conduits.…”

Section: Introductionmentioning

confidence: 99%

“…Some examples of techniques suitable to measure phloem or xylem flow are radioactive-label ( 11 C or 14 C) tracking (Minchin & Thorpe, 2003), some heat based techniques (Vandegehuchte & Steppe, 2013), fluorescent dye tracking (Jensen et al, 2011;Froelich et al, 2011) or non-invasive magnetic resonance imaging (MRI) flowmetry (Kockenberger et al, 1997;Van As, 2007). Both heat-based techniques and MRI do not directly detect transport of photo-assimilates, but mainly water (Hubeau & Steppe, 2015). However, the simultaneous measurement of phloem water transport velocity (measured with MRI) and transport velocity of a fluorescent dye (5(6)-carboxyfluorescein which could be seen as a proxy for sucrose) showed comparable velocities (Windt CW, Savage J, Knoblauch M, Holbrook M, Van As H, Unpublished results).…”

Section: Introductionmentioning

confidence: 99%

“…An integrated approach also strives to use non-invasive techniques like for example MRI, sap flow sensors using external heating, branch bags or cuvettes connected to an infrared gas analyser, and stem diameter variation sensors. Such simultaneous data acquisition of a single plant has the potential to provide unique insights, and could for example shed light on phloem-xylem interaction which remains an understudied field (Hubeau & Steppe, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Light on phloem transport (an MRI approach)

Průšová

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Section: Phloem Transportmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Light on phloem transport (an MRI approach)

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Imaging Metabolic Flow of Water in Plants with Isotope‐Traced Stimulated Raman Scattering Microscopy

Bi,

Ao,

Jiang

et al. 2024

Advanced Science

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Water plays a vital role in the life cycle of plants, participating in various critical biochemical reactions during both non‐photosynthetic and photosynthetic processes. Direct visualization of the metabolic activities of water in plants with high spatiotemporal resolution is essential to reveal the functional utilization of water. Here, stimulated Raman scattering (SRS) microscopy is applied to monitor the metabolic processes of deuterated water (D2O) in model plant Arabidopsis thaliana (A. thaliana). The work shows that in plants uptaking D2O/water solution, proton‐transfer from water to organic metabolites results in the formation of C‐D bonds in newly synthesized biomolecules (lipid, protein, and polysaccharides, etc.) that allow high‐resolution detection with SRS. Reversible metabolic pathways of oil‐starch conversion between seed germination and seed development processes are verified. Spatial heterogeneity of metabolic activities along the vertical axis of plants (root, stem, and tip meristem), as well as the radial distributions of secondary growth on the horizontal cross‐sections are quantified. Furthermore, metabolic flow of protons from plants to animals is visualized in aphids feeding on A. thaliana. Collectively, SRS microscopy has potential to trace a broad range of matter flows in plants, such as carbon storage and nutrition metabolism.

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Positron‐emitting tracer imaging of fluoride transport and distribution in tea plant

et al. 2020

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BACKGROUNDTea (Camellia sinensis (L.) O. Kuntze) is a hyper‐accumulator of fluoride (F). To understand F uptake and distribution in living plants, we visually evaluated the real‐time transport of F absorbed by roots and leaves using a positron‐emitting (18F) fluoride tracer and a positron‐emitting tracer imaging system.RESULTSF arrived at an aerial plant part about 1.5 h after absorption by roots, suggesting that tea roots had a retention effect on F, and then was transported upward mainly via the xylem and little via the phloem along the tea stem, but no F was observed in the leaves within the initial 8 h. F absorbed via a cut petiole (leaf 4) was mainly transported downward along the stem within the initial 2 h. Although F was first detected in the top and ipsilateral leaves, it was not detected in tea roots by the end of the monitoring. During the monitoring time, F principally accumulated in the node.CONCLUSIONF uptake by the petiole of excised leaf and root system was realized in different ways. The nodes indicated that they may play pivotal roles in the transport of F in tea plants. © 2020 Society of Chemical Industry

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Plant-PET Scans: In Vivo Mapping of Xylem and Phloem Functioning

Cited by 80 publications

References 84 publications

Light on phloem transport (an MRI approach)

Light on phloem transport (an MRI approach)

Imaging Metabolic Flow of Water in Plants with Isotope‐Traced Stimulated Raman Scattering Microscopy

Positron‐emitting tracer imaging of fluoride transport and distribution in tea plant

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