Plant phospholipase D: novel structure, regulatory mechanism, and multifaceted functions with biotechnological application

Deepika, Deepika; Singh, Amarjeet

doi:10.1080/07388551.2021.1924113

Cited by 23 publications

(15 citation statements)

References 153 publications

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“…Finally, and interestingly, as many as nine SNPs/InDels were found in one single gene that encodes a phospholipase D which is involved in phospholipid biosynthesis ( Li-Beisson et al, 2013 ). It has been suggested that phospholipase D is involved in the flux of fatty acids into triacylglycerols through the formation of phosphatidic acid ( Bates et al, 2020 ; Deepika and Singh, 2022 ), and the expression of a soybean phospholipase D in Arabidopsis enhanced the seed oil content ( Bai et al, 2020 ). It could be speculated that the SNPs/InDels in the gene encoding a phospholipase D could possibly be associated with the increased oil content in Pasankalla as compared to the other genotypes.…”

Section: Discussionmentioning

confidence: 99%

“…It could be speculated that the SNPs/InDels in the gene encoding a phospholipase D could possibly be associated with the increased oil content in Pasankalla as compared to the other genotypes. However, phospholipases are also involved in diverse signalling roles during plant abiotic and biotic stress responses ( Schnurbusch et al, 2019 ; Deepika and Singh, 2022 ), and more detailed studies are therefore needed to confirm any functional association of phospholipase D to oil content in quinoa.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptional Regulation of Quinoa Seed Quality: Identification of Novel Candidate Genetic Markers for Increased Protein Content

et al. 2022

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Quinoa (Chenopodium quinoa Willd.) is a crop that has great potential for increased cultivation in diverse climate regions. The seed protein quality obtained from this crop is high concerning the requirements to meet human nutritional needs, but the seed protein content is relatively low if compared to crops such as grain legumes. Increased seed protein content is desirable for increasing the economic viability of this crop in order for it to be used as a protein crop. In this study, we characterized three genotypes of quinoa with different levels of seed protein content. By performing RNA sequencing of developing seeds, we determined the genotype differences in gene expression and identified genetic polymorphisms that could be associated with increased protein content. Storage nutrient analyses of seeds of three quinoa genotypes (Titicaca, Pasankalla, and Regalona) from different ecoregions grown under controlled climate conditions showed that Pasankalla had the highest protein content (20%) and the lowest starch content (46%). Our seed transcriptome analyses revealed highly differentially expressed transcripts (DETs) in Pasankalla as compared to the other genotypes. These DETs encoded functions in sugar transport, starch and protein synthesis, genes regulating embryo size, and seed transcription factors. We selected 60 genes that encode functions in the central carbon metabolism and transcription factors as potential targets for the development of high-precision markers. Genetic polymorphisms, such as single nucleotide polymorphisms (SNPs) and base insertions and deletions (InDels), were found in 19 of the 60 selected genes, which can be further evaluated for the development of genetic markers for high seed protein content in quinoa. Increased cultivation of quinoa can contribute to a more diversified agriculture and support the plant protein diet shift. The identification of quinoa genotypes with contrasting seed quality can help establish a model system that can be used for the identification of precise breeding targets to improve the seed quality of quinoa. The data presented in this study based on nutrient and transcriptome analyses contribute to an enhanced understanding of the genetic regulation of seed quality traits in quinoa and suggest high-precision candidate markers for such traits.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptional Regulation of Quinoa Seed Quality: Identification of Novel Candidate Genetic Markers for Increased Protein Content

et al. 2022

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“…Different PLD isomers, categorized into subgroups a through z, vary in their catalytic properties and biological roles (Deepika and Singh, 2022). In this special topic, Yao et al characterize the effects of soybean PLDϵ on responses to nitrogen limitation and nitrogen-fixing rhizobacteria.…”

Section: Phospholipases and Their Productsmentioning

confidence: 99%

Editorial: Lipid metabolism and membrane structure in plant biotic interactions

2022

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“…After KEGG enrichment, we found that the genes with lower expression levels in the cr-lox1-I than in Ningjing 4 after NA included multiple genes encoding phospholipases: OsPLDζ1, OsPLDζ2, OsPLDβ1. Phospholipase D (PLD) is involved in lipid signal transduction, metabolism and degradation by converting hydrated phospholipid to non-hydrated phospholipid during seed storage and processing (Deepika and Singh, 2022). This suggests that during seed storage, phospholipase gene is induced to express, catalyzing the hydrolysis of phospholipids on cell membranes to fatty acids that are then catalyzed by OsLOX1 to harmful peroxides, resulting in seed inactivation.…”

Section: Full Textmentioning

confidence: 99%

Prolongation of seed viability and grain quality in rice by editing OsLOX1 using CRISPR/Cas9

Chen

Zhang

Tong

et al. 2022

Preprint

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Storage tolerance is an important agronomic character in rice (Oryza sativa L.). Lipoxygenase (LOX) is a key enzyme in lipid metabolism, and its activity directly affects the rate of aging in rice. Here, lipoxygenase gene OsLOX1 was edited by CRISPR/Cas9 in Ningjing 4. We found that OsLOX1 knock-out can delay seed vigor loss, lipid metabolism and quality deterioration after aging. In addition, combining the transcriptome analysis, we could conclude that OsLOX1 affected the transcription of multiple genes during storage, and was involved in seed storage tolerance by regulating lipid metabolism and antioxidant pathways such as phosphatase and acetaldehyde dehydrogenase. By knocking out LOX1 gene, a new rice material with high vigor and no exogenous vector skeleton was bred to improve the storage tolerance of seeds and provide material support for rice breeding in China.

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Plant phospholipase D: novel structure, regulatory mechanism, and multifaceted functions with biotechnological application

Cited by 23 publications

References 153 publications

Transcriptional Regulation of Quinoa Seed Quality: Identification of Novel Candidate Genetic Markers for Increased Protein Content

Transcriptional Regulation of Quinoa Seed Quality: Identification of Novel Candidate Genetic Markers for Increased Protein Content

Editorial: Lipid metabolism and membrane structure in plant biotic interactions

Prolongation of seed viability and grain quality in rice by editing OsLOX1 using CRISPR/Cas9

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