Plant Plasma Membrane Proton Pump: One Protein with Multiple Functions

Michalak, Adrianna; Wdowikowska, Anna; Janicka, Małgorzata

doi:10.3390/cells11244052

Cited by 19 publications

(5 citation statements)

References 148 publications

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“…PM ATPase activity has been reported to be altered by drought in many plant species [ 49 , 50 ]. In our study, PM ATPase activity is significantly reduced by drought stress, a reduction that has been previously reported by other investigators [ 51 , 52 ]. These findings are also in agreement with the results of Mi et al [ 53 ], who showed that Na + , K + -ATPase, and Ca 2+ , Mg 2+ -ATPase activities of winter rapeseed leave cells gradually decreased with increasing drought severity.…”

Section: Discussionsupporting

confidence: 91%

Proline Enhances Resistance and Recovery of Oilseed Rape after a Simulated Prolonged Drought

Jurkonienė

Mockevičiūtė

Gavelienė

et al. 2023

Plants

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This study was carried out to evaluate the effect of exogenous proline on the growth, biochemical responses, and plant recovery of drought-stressed oilseed rape plants after renewed irrigation. The experiment was conducted under controlled laboratory conditions. After 21 days of cultivation, 3–4 leaf stage seedlings were sprayed with proline (1 mM), then subjected to prolonged drought stress for 8 days to achieve a severe water deficit, next, irrigation was resumed and recovery was assessed after 4 days. The results show that exogenous application of proline reduced the drought-induced growth inhibition of seedlings while maintaining relative water content (RWC) and growth parameters closer to those of irrigated plants. Proline had a positive effect on chlorophyll accumulation and membrane permeability while decreasing ethylene, H2O2, and MDA levels. Moreover, after 4 days of recovery, the H2O2 content of the proline-treated plants was significantly lower (2-fold) and the MDA content was close to that of continuously irrigated plants. Thus, all these biochemical reactions influenced plant survival: after drought + proline treatment, the number of surviving plants was two times higher than that of drought-treated plants. The findings show that exogenous proline has antioxidant, osmotic, and growth-promoting properties that improve the drought tolerance of winter oilseed rape plants and is, therefore, beneficial for drought adaptation in oilseed rape.

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Section: Discussionsupporting

confidence: 91%

Proline Enhances Resistance and Recovery of Oilseed Rape after a Simulated Prolonged Drought

Jurkonienė

Mockevičiūtė

Gavelienė

et al. 2023

Plants

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“…Moreover, K + uptake under low K + conditions is promoted by the direct interaction between AHA2 and the receptor-like protein kinase BAK1 (Wang et al, 2022). Since the electrical membrane potential and chemical pH gradient generated by the proton-pumping ATPase AHA2 are essential for the function of the majority of plant PM transporters, it is not surprising that AHA2 is regulated by and interacting with many physiological parameters and protein factors (reviewed by Falhof et al, 2016;Michalak et al, 2022). AHA2 expression is affected by changing nitrate supply (Młodzińska et al, 2015) and phosphorus deficiency (Yuan et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

The K+ transporter NPF7.3/NRT1.5 and the proton pump AHA2 contribute to K+ transport in Arabidopsis thaliana under K+ and NO3- deficiency

Sena,

Kunze

2023

Front. Plant Sci.

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Nitrate (NO3-) and potassium (K+) are distributed in plants via short and long-distance transport. These two pathways jointly regulate NO3- and K+ levels in all higher plants. The Arabidopsis thaliana transporter NPF7.3/NRT1.5 is responsible for loading NO3- and K+ from root pericycle cells into the xylem vessels, facilitating the long-distance transport of NO3- and K+ to shoots. In this study, we demonstrate a protein-protein interaction of NPF7.3/NRT1.5 with the proton pump AHA2 in the plasma membrane by split ubiquitin and bimolecular complementation assays, and we show that a conserved glycine residue in a transmembrane domain of NPF7.3/NRT1.5 is crucial for the interaction. We demonstrate that AHA2 together with NRT1.5 affects the K+ level in shoots, modulates the root architecture, and alters extracellular pH and the plasma membrane potential. We hypothesize that NRT1.5 and AHA2 interaction plays a role in maintaining the pH gradient and membrane potential across the root pericycle cell plasma membrane during K+ and/or NO3- transport.

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“…In plants, the sodium pump is replaced by a P-type proton pump of similar structure and mechanism, powered by ATP hydrolysis to generate an electrochemical proton gradient that powers transport of ions and substrates across the plasma membrane ( Michalak et al, 2022 ). The plant vacuole is powered by V-type proton ATPases and by a pyrophosphatase-hydrolysis coupled proton pump ( Harrison and Muench, 2018 ).…”

Section: Bioenergetic Myth 2 Atp Use = Energy Consumptionmentioning

confidence: 99%

Bioenergetic myths of energy transduction in eukaryotic cells

Brown

2024

Front. Mol. Biosci.

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The study of energy transduction in eukaryotic cells has been divided between Bioenergetics and Physiology, reflecting and contributing to a variety of Bioenergetic myths considered here: 1) ATP production = energy production, 2) energy transduction is confined to mitochondria (plus glycolysis and chloroplasts), 3) mitochondria only produce heat when required, 4) glycolysis is inefficient compared to mitochondria, and 5) mitochondria are the main source of reactive oxygen species (ROS) in cells. These myths constitute a ‘mitocentric’ view of the cell that is wrong or unbalanced. In reality, mitochondria are the main site of energy dissipation and heat production in cells, and this is an essential function of mitochondria in mammals. Energy transduction and ROS production occur throughout the cell, particularly the cytosol and plasma membrane, and all cell membranes act as two-dimensional energy conduits. Glycolysis is efficient, and produces less heat per ATP than mitochondria, which might explain its increased use in muscle and cancer cells.

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Plant Plasma Membrane Proton Pump: One Protein with Multiple Functions

Cited by 19 publications

References 148 publications

Proline Enhances Resistance and Recovery of Oilseed Rape after a Simulated Prolonged Drought

Proline Enhances Resistance and Recovery of Oilseed Rape after a Simulated Prolonged Drought

The K+ transporter NPF7.3/NRT1.5 and the proton pump AHA2 contribute to K+ transport in Arabidopsis thaliana under K+ and NO3- deficiency

Bioenergetic myths of energy transduction in eukaryotic cells

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