2017
DOI: 10.3791/56140
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Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Abstract: The upstream sequences of gene coding sequences are termed as promoter sequences. Studying the expression patterns of promoters are very significant in understanding the gene regulation and spatiotemporal expression patterns of target genes. On the other hand, it is also critical to establish promoter evaluation tools and genetic transformation techniques that are fast, efficient, and reproducible. In this study, we investigated the spatiotemporal expression pattern of the rhizobial symbiosis-specific nodule i… Show more

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Cited by 12 publications
(13 citation statements)
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“…Previously, we performed P. vulgaris RNA-seq analysis of mRNA from uninoculated control and 2 weeks post inoculation with mycorrhized or nodulated roots using Ion Proton sequencing; data obtained were then deposited in the NCBI with accession number of PRJNA388751 (). In total, 1959 upregulated and 1260 downregulated mycorrhized DEGs and 1247 upregulated and 1398 downregulated nodulated DEGs were identified [47]. Herein, we validated the RNA-Seq results.…”
Section: Resultsmentioning
confidence: 72%
See 1 more Smart Citation
“…Previously, we performed P. vulgaris RNA-seq analysis of mRNA from uninoculated control and 2 weeks post inoculation with mycorrhized or nodulated roots using Ion Proton sequencing; data obtained were then deposited in the NCBI with accession number of PRJNA388751 (). In total, 1959 upregulated and 1260 downregulated mycorrhized DEGs and 1247 upregulated and 1398 downregulated nodulated DEGs were identified [47]. Herein, we validated the RNA-Seq results.…”
Section: Resultsmentioning
confidence: 72%
“…To validate the RNA-seq data, we surface-sterilized P. vulgaris L. cv. Negro Jamapa seeds and germinated them as described by Nanjareddy et al [47]. Two-day-old germinated seedlings were transplanted into sterile vermiculite and were inoculated with R. irregularis or R. tropici according to Nanjareddy et al [46].…”
Section: Methodsmentioning
confidence: 99%
“…Because gene expression is driven in specific tissues and development stages, tissue-specific promoters, also called organ- or cell-specific promoters [ 13 ], are different from other promoters [ 14 ]. These promoters are significant because they avoid potential negative effects of using constitutive promoters, such as metabolic burden [ 15 , 16 , 17 , 18 , 19 ].…”
Section: Introductionmentioning
confidence: 99%
“…To generate the BYPASS1 promoter: β-glucuronidase (GUS) construct, a 1154-bp fragment of PvBPS1.1 and 1124 bp of PvBPS1.2 promoters that were upstream of the translation initiation codons were identified and isolated from P. vulgaris genomic DNA, according to Nanjareddy et al [ 33 ]. The PCR fragments were cloned separately into the pENTR/D-TOPO vector (Thermo Fisher Scientific, Waltham, MA, USA) and recombined into the destination binary vector pBGWSF7.0 [ 34 ] according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA).…”
Section: Methodsmentioning
confidence: 99%
“…The correct orientations of the above clones were confirmed by sequencing the plasmid insert. The recombinant plasmids were introduced into Agrobacterium rhizogenes strain K599, and then transformed into P. vulgaris roots using the rapid hairy root transformation method, as described recently by Nanjareddy and associates [ 33 ].…”
Section: Methodsmentioning
confidence: 99%