Plant protein P3IP participates in the regulation of autophagy in <i>Nicotiana benthamiana</i>

Jiang, Liangliang; Zheng, Xiying; Liu, Yule; Chen, Jianping; Lu, Yuwen; Yan, Fei

doi:10.1080/15592324.2020.1861768

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…Cotton leaf curl multan virus bC1 induces autophagy by disrupting the interaction of ATG3 with glyceraldehyde‐3‐phosphate dehydrogenases (Ismayil et al, 2020 ). N. benthamiana P3IP protein interacts with rice stripe virus p3 protein and mediates its autophagic degradation to limit viral infection (Jiang et al, 2021 ). The best way for virus and host to coexist is long‐term parasitic coexistence, and the regulation of host resistance response is an important part of long‐term parasitic virus.…”

Section: Discussionmentioning

confidence: 99%

Tomato chlorosis virus p22 interacts with NbBAG5 to inhibit autophagy and regulate virus infection

Shang

Xiao

Zhang

et al. 2023

Molecular Plant Pathology

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Tomato chlorosis virus (ToCV) is a member of the genus Crinivirus in the family Closteroviridae . It has a wide host range and wide distribution, causing serious harm to the vegetable industry. The autophagy pathway plays an important role in plant resistance to virus infection. Viruses and plant hosts coevolve in defence and antidefence processes around autophagy. In this study, the interaction between ToCV p22 and Nicotiana benthamiana B‐cell lymphoma2‐associated athanogenes5 Nicotiana benthamiana (NbBAG5) was examined. Through overexpression and down‐regulation of NbBAG5, results showed that NbBAG5 could negatively regulate ToCV infection. NbBAG5 was found to be localized in mitochondria and can change the original localization of ToCV p22, which is colocalized in mitochondria. NbBAG5 inhibited the expression of mitophagy‐related genes and the number of autophagosomes, thereby regulating viral infection by affecting mitophagy. In summary, this study demonstrated that ToCV p22 affects autophagy by interacting with NbBAG5, established the association between viral infection, BAG proteins family, and the autophagy pathway, and explained the molecular mechanism by which ToCV p22 interacts with NbBAG5 to inhibit autophagy to regulate viral infection.

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Section: Discussionmentioning

confidence: 99%

Tomato chlorosis virus p22 interacts with NbBAG5 to inhibit autophagy and regulate virus infection

Shang

Xiao

Zhang

et al. 2023

Molecular Plant Pathology

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“…Similarly, the rice homolog OsP3IP also mediates p3 degradation via interaction with OsATG8b and p3 ( Jiang et al, 2021a ). When CFP-NbATG8f was co-expressed with NbP3IP-Myc in Nicotiana benthamiana , an increase in CFP -labeled autophagic structures was detected, indicating that P3IP participates in the regulation of autophagy during the plant viral defense response ( Jiang et al, 2021b ). In Arabidopsis , a small 71 amino acid peptide, VISP1, can be induced by Cucumber mosaic virus (CMV) infection and interacts with ATG8 through UIM (ubiquitin-interacting motif) motif.…”

Section: Regulation Of Autophagy By Biotic Stress Signalsmentioning

confidence: 99%

“…Under prolonged carbon (C) starvation, ATG6 can be phosphorylated by SnRK1 to initiate autophagy via an ATG1-independent pathway. defense response (Jiang et al, 2021b). In Arabidopsis, a small 71 amino acid peptide, VISP1, can be induced by Cucumber mosaic virus (CMV) infection and interacts with ATG8 through UIM (ubiquitin-interacting motif) motif.…”

Section: Regulation Of Autophagy By Biotic Stress Signalsmentioning

confidence: 99%

Plant Autophagy: An Intricate Process Controlled by Various Signaling Pathways

Wang

Han

et al. 2021

Front. Plant Sci.

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Autophagy is a ubiquitous process used widely across plant cells to degrade cellular material and is an important regulator of plant growth and various environmental stress responses in plants. The initiation and dynamics of autophagy in plant cells are precisely controlled according to the developmental stage of the plant and changes in the environment, which are transduced into intracellular signaling pathways. These signaling pathways often regulate autophagy by mediating TOR (Target of Rapamycin) kinase activity, an important regulator of autophagy initiation; however, some also act via TOR-independent pathways. Under nutrient starvation, TOR activity is suppressed through glucose or ROS (reactive oxygen species) signaling, thereby promoting the initiation of autophagy. Under stresses, autophagy can be regulated by the regulatory networks connecting stresses, ROS and plant hormones, and in turn, autophagy regulates ROS levels and hormone signaling. This review focuses on the latest research progress in the mechanism of different external signals regulating autophagy.

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Silencing of the calcium-dependent protein kinase TaCDPK27 improves wheat resistance to powdery mildew

et al. 2023

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Background Calcium ions (Ca2+), secondary messengers, are crucial for the signal transduction process of the interaction between plants and pathogens. Ca2+ signaling also regulates autophagy. As plant calcium signal-decoding proteins, calcium-dependent protein kinases (CDPKs) have been found to be involved in biotic and abiotic stress responses. However, information on their functions in response to powdery mildew attack in wheat crops is limited. Result In the present study, the expression levels of TaCDPK27, four essential autophagy-related genes (ATGs) (TaATG5, TaATG7, TaATG8, and TaATG10), and two major metacaspase genes, namely, TaMCA1 and TaMCA9, were increased by powdery mildew (Blumeria graminis f. sp. tritici, Bgt) infection in wheat seedling leaves. Silencing TaCDPK27 improves wheat seedling resistance to powdery mildew, with fewer Bgt hyphae occurring on TaCDPK27-silenced wheat seedling leaves than on normal seedlings. In wheat seedling leaves under powdery mildew infection, silencing TaCDPK27 induced excess contents of reactive oxygen species (ROS); decreased the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT); and led to an increase in programmed cell death (PCD). Silencing TaCDPK27 also inhibited autophagy in wheat seedling leaves, and silencing TaATG7 also enhanced wheat seedling resistance to powdery mildew infection. TaCDPK27-mCherry and GFP-TaATG8h colocalized in wheat protoplasts. Overexpressed TaCDPK27-mCherry fusions required enhanced autophagy activity in wheat protoplast under carbon starvation. Conclusion These results suggested that TaCDPK27 negatively regulates wheat resistance to PW infection, and functionally links with autophagy in wheat.

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Plant protein P3IP participates in the regulation of autophagy in Nicotiana benthamiana

Cited by 4 publications

References 19 publications

Tomato chlorosis virus p22 interacts with NbBAG5 to inhibit autophagy and regulate virus infection

Tomato chlorosis virus p22 interacts with NbBAG5 to inhibit autophagy and regulate virus infection

Plant Autophagy: An Intricate Process Controlled by Various Signaling Pathways

Silencing of the calcium-dependent protein kinase TaCDPK27 improves wheat resistance to powdery mildew

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