Plant Regeneration from Anther Culture of Panax ginseng

Abstract

-The research concerned of the regeneration of plants from embryos obtained from anther cultures of ginseng (Panax ginseng C. A. Meyer). The aim was to determine the influence of the regeneration medium on the efficiency of the regeneration process. We conducted to determine the optimum conditions such as cold pretreatment, plant growth regulators and carbon sources on anther culture of P. ginseng. Highest callus formation rate was obtained when flower buds pretreated at 4℃ for 1 day. Among the treated growth regulators with various degrees of concentration in Murashige and Skoog`s (MS) medium, 4.53 μM of 2.4-dichlorophenoxyacetic acid and 4.44 μM of 6-benzylaminopurine gives the most responsive callus with the frequency of 73.89% and 129.53 g of fresh weight. When we used 3-9% of sucrose and maltose among the different kinds and various concentrations of carbohydrates, callus was formed highest 67.29% in the medium with 3% of sucrose. Shoots induced from callus supplemented with 28.9 μM of gibberellic acid and rooted in Gamborg`s B5 medium supplemented with 14.7 μM of indole-3-butyric acid.

In Vitro Cultivation and Ginsenosides Accumulation in Panax ginseng: A Review

“…In addition, under controlled culture conditions, numerous factors influence the quantity and quality of ginsenosides, such as medium constituents, pH, light conditions, culture temperature, explants, and abiotic factors. [32], (b) somatic embryos (bar 1 mm) [33], (c) shoots (bar 1 cm) [32], (d) hairy roots (bar 820 µm) [34], (e) cell suspension [35], and (f) adventitious roots [36]. [32], (b) somatic embryos (bar 1 mm) [33], (c) shoots (bar 1 cm) [32], (d) hairy roots (bar 820 µm) [34], (e) cell suspension [35], and (f) adventitious roots [36].…”

“…[32], (b) somatic embryos (bar 1 mm) [33], (c) shoots (bar 1 cm) [32], (d) hairy roots (bar 820 µm) [34], (e) cell suspension [35], and (f) adventitious roots [36]. [32], (b) somatic embryos (bar 1 mm) [33], (c) shoots (bar 1 cm) [32], (d) hairy roots (bar 820 µm) [34], (e) cell suspension [35], and (f) adventitious roots [36].…”

Embryogenesis and plant regeneration of Panax ginseng Meyer via anther culture and ploidy assessment using flow cytometry

“…이전의 인삼 약 배양 연구는 캘러스 유도, 식물 재분화 및 4배체 부정근 형성만을 보고하였으며, 배 발생에 대한 결과 는 없었다 (Lee et al 2009;Lee et al 2013). 인삼은 종자 내 자엽 을 이용한 체세포배 발생 연구는 많이 이루어졌지만 (Choi et al 1999;Kim et al 2012;Lee et al 2019)…”

Biotechnological Interventions for Ginsenosides Production

“…The utilization of auxins in the basal medium leads to the formation of friable calli. The process of regeneration of plantlets from callus is effectuated by the addition of gibberellic acid (GA 3 ) and 6-benzylaminopurine (BAP) in the basal medium [23,24]. Compounds like picloram or dicamba can also be used to induce callus from an array of explants, since they possess auxin-mimicking activity.…”