Plant Regeneration from in vitro Cultured Anthers of Black Mustard (Brassica nigra Koch)

Govil, Suman; Babbar, Shashi B.; Gupta, Sudhiranjan

doi:10.1111/j.1439-0523.1986.tb01302.x

Cited by 16 publications

(4 citation statements)

References 14 publications

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“…Microspore culture of recalcitrant cruciferous species The use of PEG or sucrose in the NLN culture medium was compared in a number of cruciferous species (i.e., B. alboglabra, B. carinata, B. juncea, B. nigra, B. rapa, C. abyssinica, R. oleifera, and S. alba). There have been very few or no published protocols for some of these species (e.g., B. nigra, C. abyssinica, R. oleifera, and S. alba; Klimaszewska and Keller 1983;Govil et al 1986;Hetz and Shieder 1989;Takahata et al 1996). Embryos and doubled haploid plants were produced in all eight species evaluated (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Optimization of methods for using polyethylene glycol as a non-permeating osmoticum for the induction of microspore embryogenesis in the Brassicaceae

Ferrie

Keller

2007

In Vitro Cell.Dev.Biol.-Plant

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The objective of this work was to enhance the quality and quantity of microspore-derived embryos of cruciferous species by using polyethylene glycol (PEG) to replace sucrose in the culture medium. The main advantage in using PEG is that it produces embryos that are morphologically more similar to zygotic embryos and have enhanced germination capabilities. When microspores were cultured in full strength NLN medium supplemented with 25% (w/v) PEG, the addition of 3 ml of full strength NLN with 13% (w/v) sucrose at 14 d was beneficial for embryo quality and quantity. Experiments showed that this PEG system could be used for a number of Brassica napus cultivars, as well as a number of other cruciferous species. PEG enhanced microspore embryogenesis in B. nigra, Crambe abyssinica, and Raphanus oleifera. Microsporederived embryos were obtained from all cruciferous species evaluated (B. alboglabra, B. carinata, B. juncea, B. rapa, B. nigra, R. oleifera, Crambe abyssinica, Sinapis alba) using either sucrose or PEG as the osmoticum. Microspore embryogenesis was induced in B. napus in PEG-based cultures without a 32°C heat shock (i.e., 4, 15, 18, and 24°C). These temperature conditions were non-inductive when sucrose was used as the osmoticum. Spontaneous chromosome doubling occurred in 64-92% of the regenerated plants when PEG was used in the NLN culture medium, whereas in culture medium containing sucrose, the spontaneous doubling rate was 2-18%.

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Section: Resultsmentioning

confidence: 99%

Optimization of methods for using polyethylene glycol as a non-permeating osmoticum for the induction of microspore embryogenesis in the Brassicaceae

Ferrie

Keller

2007

In Vitro Cell.Dev.Biol.-Plant

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“…Androgenic haploids, through direct pollen grain embryogenesis, have been reported for most crop species of Brassica (Arora & Bhojwani, 1988;Chiang et al, 1985;Govil et al, 1986;Keller et al, 1975;Keller & Armstrong, 1977), including B. juncea (George & Rao, 1982;Sharma & Bhojwani, 1985). However, the androgenic response of B. juncea has been extremely poor (< 4%).…”

Section: Introductionmentioning

confidence: 99%

Enhanced pollen grain embryogenesis and plant regeneration in anther cultures of Brassica juncea cv. PR-45

Agarwal

Bhojwani

1993

Euphytica

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Pollen grain embryogenesis in anther cultures of Brassica juncea cv. PR-45 was considerably enhanced by treating the donor plants with 4 ml 1-1 (v/v) of ethrel or delayed sowing of the donor plants, the latter treatment being superior. The anthers derived from plants sown about two months after the normal sowing period showed 18% androgenesis as compared to 3.5% in the control.Pollen grain embryos normally showed very poor germination (10 %) on B 5 or B 5 containing GA 3. However, ABA or cold treatment promoted normal germination of these embryos. Exposure of the embryos to 4 ° C for 6 days, which proved to be the best treatment, induced 66% germination of the embryos.

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“…In this species, detailed protocols have been worked out for regenerating plantlets from anther and pollen cultures [12,17,25,26], protoplast-derived cell colonies [10,16,24] and a number of somatic tissue explants [14]. In addition, tissue culture studies have also been done in B. campestris [13,21], B. carinata [2,3], B. juncea [7,9,22], B. nigra [11] and B. oleracea [8], but these have involved only a few cultivars. The present paper reports the results of a detailed study undertaken to work out the conditions for controlled high-frequency plant regeneration from cotyledonary explant calli of Indian cultivars of B. juncea, B. campestris and B. carinata.…”

Section: Introductionmentioning

confidence: 99%

Genotypic and media effects on plant regeneration from cotyledon explant cultures of someBrassica species

Jain

Chowdhury

Sharma

et al. 1988

Plant Cell Tiss Organ Cult

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Conditions were established for efficient plant regeneration from cotyledon explant calli in different cultivars of Brassica juncea, B. campestris and B. carinata on Murashige & Skoog's (MS) medium supplemented with various combinations of cytokinins and auxins.Regeneration frequency, however, varied with genotype and the different growth hormone combinations in media. Almost in all species, MS medium with zeatin (1.0mg 1 -t) and IAA (0.1 mg 1-t ) was found to be best for shoot organogenesis followed by the ones containing high kinetin (2.0mg 1 -t) and low IAA (0.02 or 0.2mg l -t) concentrations. On these media, the cotyledonary explants invariably underwent callusing followed by multiple shoot formation, which could be separated and subcultured for further propagation. Number of shoots per cotyledon explant cultured varied from 0 to as many as 50. In B. juncea and B. campestris, the regeneration frequency declined sharply in the absence of auxin in medium. BAP in combination with NAA yielded no or a reduced number of shoots. Shoot organogenesis also declined with the reduction in photoperiod from continuous light to 16 hours. Shoots were easily rooted during prolonged incubation on the same medium and whole plants were transferred to pots in the greenhouse and grown to maturity.

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Plant Regeneration from in vitro Cultured Anthers of Black Mustard (Brassica nigra Koch)

Cited by 16 publications

References 14 publications

Optimization of methods for using polyethylene glycol as a non-permeating osmoticum for the induction of microspore embryogenesis in the Brassicaceae

Optimization of methods for using polyethylene glycol as a non-permeating osmoticum for the induction of microspore embryogenesis in the Brassicaceae

Enhanced pollen grain embryogenesis and plant regeneration in anther cultures of Brassica juncea cv. PR-45

Genotypic and media effects on plant regeneration from cotyledon explant cultures of someBrassica species

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