Plant regeneration from mesophyll protoplasts of a medicinal plant, Phellodendron amurense rupr.

Azad, Mustafa Abul Kalam; Yokota, Shinso; Ishiguri, Futoshi; Yoshizawa, Nobuo

doi:10.1079/ivp2006808

Cited by 14 publications

(7 citation statements)

References 17 publications

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“…The lowest performance was shown in Kn plus IBA or NAA formulations. Similar results were found in leaf and stem nodes explants of Lilium (Bacchetta et al 2003), callus derived protoplast in Phellodendron amurense (Azad 2012), cotyledon explant in Acacia mangium , epicotyl and internode explants in Cajanus cajan . Our study also revealed that BAP-IBA and BAP-NAA formulation was better than Kn-IBA or Kn-NAA formulations.…”

Section: Resultssupporting

confidence: 80%

Regeneration of Asparagus officinalis L. Through Embryogenic Callus

Azad

Amin

2017

Plant Tissue Cult. & Biotech.

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Section: Resultssupporting

confidence: 80%

Regeneration of Asparagus officinalis L. Through Embryogenic Callus

Azad

Amin

2017

Plant Tissue Cult. & Biotech.

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“…Berberine is an active compound of P. amurense found in relatively high concentrations which has been shown to possess the antimicrobial [23] and anti-inflammatory [24] properties. While the bark of P. amurense contains abundant cellulose [25], whether or not berberine-enriched CMC can be fabricated from the bark of P. amurense has not yet been determined.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Novel Hydrogel with Berberine-Enriched Carboxymethylcellulose and Hyaluronic Acid as an Anti-Inflammatory Barrier Membrane

Huang

Yang

et al. 2016

BioMed Research International

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An antiadhesion barrier membrane is an important biomaterial for protecting tissue from postsurgical complications. However, there is room to improve these membranes. Recently, carboxymethylcellulose (CMC) incorporated with hyaluronic acid (HA) as an antiadhesion barrier membrane and drug delivery system has been reported to provide excellent tissue regeneration and biocompatibility. The aim of this study was to fabricate a novel hydrogel membrane composed of berberine-enriched CMC prepared from bark of the P. amurense tree and HA (PE-CMC/HA). In vitro anti-inflammatory properties were evaluated to determine possible clinical applications. The PE-CMC/HA membranes were fabricated by mixing PE-CMC and HA as a base with the addition of polyvinyl alcohol to form a film. Tensile strength and ultramorphology of the membrane were evaluated using a universal testing machine and scanning electron microscope, respectively. Berberine content of the membrane was confirmed using a UV-Vis spectrophotometer at a wavelength of 260 nm. Anti-inflammatory property of the membrane was measured using a Griess reaction assay. Our results showed that fabricated PE-CMC/HA releases berberine at a concentration of 660 μg/ml while optimal plasticity was obtained at a 30 : 70 PE-CMC/HA ratio. The berberine-enriched PE-CMC/HA had an inhibited 60% of inflammation stimulated by LPS. These results suggest that the PE-CMC/HA membrane fabricated in this study is a useful anti-inflammatory berberine release system.

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“…Moreover, myo-inositol protects plants against salt stress and is suggested to function in salt tolerance in two major ways: to protect cellular structures from reactive oxidizers and to control the water pressure inside cells (Loewua and Murthy, 2000). In addition, myo-inositol acts as a growth enhancer in vitro and a carbohydrate source, which are good osmotica for sustained cell division (Azad et al, 2006;Eun et al, 2011). Therefore, myo-inositol is considered an important factor for increasing the callus induction rate in strawberry anther culture.…”

Section: Callus Induction Based On Culture Medium Type Myoinositol mentioning

confidence: 99%

Anther-derived callus induction based on culture medium, myo-inositol, AgNO3 and Fe-EDTA in Seolhyang strawberries

Na¹,

Kim²,

Kim³

2019

J. Plant Breed. Crop Sci.

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Haploid breeding is an effective method of shortening the breeding period of plants. In this study, to develop a haploid breeding technique, optimum conditions of the culture medium were investigated for anther-derived callus induction in the strawberry cultivar Seolhyang. The effects of the culture medium type, myo-inositol, auxin and cytokinin combination treatment, silver nitrate (AgNO 3), and ferric ethylenediaminetetraacetic acid (Fe-EDTA) on anther-derived callus induction were analyzed. Anthers were incubated in Murashige and Skoog (MS) medium, Gamborg B5 medium (B5), and Lichter medium (NLN) for 8 weeks. Each culture medium had 0.4 mg•L-1 of 6-benzyladenine (BA), 0.1 mg•L −1 of indole-3 acetic acid (IAA), and 2.0 mg•L −1 of 2,4-dichlorophenoxyacetic acid (2,4-D) added to it. Results showed that MS medium was most effective in callus induction. When 100 mg•L −1 of myo-inositol was added to each medium, the callus induction rate increased. Auxin and cytokinin combination treatment was more effective with the addition of 0.4 mg•L −1 of BA, 0.1 mg•L −1 of IAA, and 2.0 mg•L −1 of 2,4-D to MS medium compared to the addition of 0.1 mg•L −1 of BA, 2.0 mg•L −1 of IAA, and 0.4 mg•L −1 of 2,4-D. With AgNO 3 treatment, the highest callus induction rate was found at a concentration of 25 mg•L −1. The callus induction rate increased as the AgNO 3 concentration increased; however, a significant decrease was seen at 30 mg•L −1. In the case of Fe-EDTA, the most effective concentration for callus induction was 25 mg•L −1. Therefore, supplementing myo-inositol, AgNO 3 and Fe-EDTA can help in anther-derived callus induction in Seolhyang strawberries.

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Plant regeneration from mesophyll protoplasts of a medicinal plant, Phellodendron amurense rupr.

Cited by 14 publications

References 17 publications

Regeneration of Asparagus officinalis L. Through Embryogenic Callus

Regeneration of Asparagus officinalis L. Through Embryogenic Callus

Fabrication of Novel Hydrogel with Berberine-Enriched Carboxymethylcellulose and Hyaluronic Acid as an Anti-Inflammatory Barrier Membrane

Anther-derived callus induction based on culture medium, myo-inositol, AgNO3 and Fe-EDTA in Seolhyang strawberries

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