Regeneration of Asparagus officinalis L. Through Embryogenic Callus

Azad, Mustafa Abul Kalam; Amin, Muhammad Nurul

doi:10.3329/ptcb.v27i1.35008

Cited by 5 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, for NAA + KN there was lower percentage of response. Present findings corroborate with the findings of Gyulai et al, (1992), Kohmura et al, (1994), Mustapha et al, (1996), Sharma et al, (2011), Dezfuli et al, (2013), Patel and Patel (2015), Azad et al, (2017), Pank andJoshi (2017), Lamor et al, (2018), and Chaudhary and Dantu (2019). These workers induced calli for morphogenetic studies.…”

Section: Discussionsupporting

confidence: 90%

TISSUE CULTURE STUDY FOR EFFICIENT CALLUS INDUCTION FROM INTERNODAL EXPLANTS OF Asparagus racemosus WILLD AN IMPORTANT MEDICINAL HERB

Kumari¹,

Kumari²,

Singh³

2020

Indian Journal of Scientific Research

View full text Add to dashboard Cite

Tissue culture study was performed to standardize the technique and select suitable plant growth regulators and their concentrations for efficient callus induction from the nodal explants of Asparagus racemosus willd, an important medicinal herb. Different concentrations of NAA, 2,4-D, BAP and KN were supplemented in MS basal medium in which the nodal explants were inoculated. It was observed that MS + 0.2 mg/l NAA was the best culture condition in which maximum percentage of response 90.36 was obtained. This was followed by the culture conditions that were MS+ 2.0 mg/l where the percentage response was 88.68%. Similarly, next higher percentage 88.62 was observed in MS+1.0 mg/l BAP + 1.0 mg/l NAA. It was further observed that growth rate was excellent where the percentage response was higher. Second it was noted that the calli had both compact and friable texture and their colour varied from green yellow to brown even where the growth was excellent. It was further noted that NAA or BAP had no response for callusing when used alone. However, KN at higher concentration 2.0 mg/l gave the highest percentage of response for callusing. NAA + BAP and NAA + KN at the similar concentrations gave different result. In the MS medium having 1.0 mg/l BAP + 1.0 mg/l NAA the percentage of response was 88.62 while MS + 1.0 mg/l KN + 1.0 mg/l NAA the percentage of response was 62.34 only. Growth rate of the calli also differed in different culture conditions.

show abstract

Section: Discussionsupporting

confidence: 90%

TISSUE CULTURE STUDY FOR EFFICIENT CALLUS INDUCTION FROM INTERNODAL EXPLANTS OF Asparagus racemosus WILLD AN IMPORTANT MEDICINAL HERB

Kumari¹,

Kumari²,

Singh³

2020

Indian Journal of Scientific Research

View full text Add to dashboard Cite

show abstract

“…The source, age, and amount of endogenous phytohormones in the explant, as well as the type, concentration, and action mode of the auxin and cytokinin hormone used, may be very important for the formation of calli [28,29]. Similarly, in Asparagus officinalis [30], Betula platyphylla [31], Coriandrum sativum [21], and Notopterygium incisum [32], the addition of a low concentration of BA to an MS medium supplemented with 2,4-D induced efficient callus formation.…”

Section: Discussionmentioning

confidence: 99%

Callus-Mediated High-Frequency Plant Regeneration, Phytochemical Profiling, Antioxidant Activity and Genetic Stability in Ruta chalepensis L.

Qahtan

Faisal

Alatar

et al. 2022

Plants

View full text Add to dashboard Cite

Efficient methods for callus induction and the high-frequency plant regeneration of Ruta chalepensis L. were established, and the phytochemical potential and antioxidant activity of a donor plant, ex-vitro-established micropropagated plants, and callus were also studied. Yellowish-green callus was induced with a frequency of 97.8% from internode shoot segments of the donor plant growing in soil in the botanical garden cultured on Murashige and Skoog (MS) medium containing 10 μM 2,4-D (2,4-dichlorophenoxyacetic acid) and 1 μM BA (6-benzyladenine). Adventitious shoots were regenerated from the yellowish-green callus on MS medium containing 5.0 μM (BA) and 1.0 μM 1-naphthaleneacetic acid (NAA), with a regeneration frequency of 98.4% and a maximum of 54.6 shoots with an average length of 4.5 cm after 8 weeks. The regenerated shoots were rooted in a medium containing 1.0 μM IBA (indole-3-butyric acid) and successfully transferred to ex vitro conditions in pots containing normal garden soil, with a 95% survival rate. The amounts of alkaloids, phenolics, flavonoids, tannins, and antioxidant activity of the ex-vitro-established micropropagated plants were higher than in the donor plant and callus. The highest contents of hesperidin and rutin (93.3 and 55.9 µg/mg, respectively) were found in the ex-vitro-established micropropagated plants compared to those obtained from the donor plant (91.4 and 31.0 µg/mg, respectively) and callus (59.1 and 21.6 µg/mg, respectively). The genetic uniformity of the ex-vitro-established micropropagated plants was appraised by the ISSR markers and compared with the donor plant. This is the first report describing the callus-mediated plant regeneration, as well as the production of phenolic compounds and antioxidant activities in R. chalepensis, which might be a potential alternative technique for the mass propagation and synthesis of bioactive compounds such as hesperidin and rutin.

show abstract

Histological assessment of regenerating plants at callus, shoot organogenesis and plantlet stages during the in vitro micropropagation of Asparagus cochinchinensis

Kim

Okello

Yang

et al. 2020

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Regeneration of Asparagus officinalis L. Through Embryogenic Callus

Abstract: A plant regeneration system was established from hypocotyl explants of in vitro grown seedlings of A. officinalis and in vitro proliferated shoots, respectively through somatic embryogenesis and embryogenic calli.

Cited by 5 publications

References 11 publications

TISSUE CULTURE STUDY FOR EFFICIENT CALLUS INDUCTION FROM INTERNODAL EXPLANTS OF Asparagus racemosus WILLD AN IMPORTANT MEDICINAL HERB

TISSUE CULTURE STUDY FOR EFFICIENT CALLUS INDUCTION FROM INTERNODAL EXPLANTS OF Asparagus racemosus WILLD AN IMPORTANT MEDICINAL HERB

Callus-Mediated High-Frequency Plant Regeneration, Phytochemical Profiling, Antioxidant Activity and Genetic Stability in Ruta chalepensis L.

Histological assessment of regenerating plants at callus, shoot organogenesis and plantlet stages during the in vitro micropropagation of Asparagus cochinchinensis

Contact Info

Product

Resources

About