Plant regeneration from suspension cells induced from hypocotyls derived from interspecific cross Alstroemeria pelegrina × A. magenta and transformation with Agrobacterium tumefaciens

Hoshino, Yoichiro; Kashihara, Yukiko; Hirano, Tomonari; Murata, Naho; Shinoda, Koichi

doi:10.1007/s11240-008-9385-x

Cited by 4 publications

(4 citation statements)

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“…1e) and distinguishable perianth shapes are considered to be useful for the analysis of floral zygomorphy. Furthermore, an Agrobacterium-mediated transformation system has now been established in Alstroemeria (Kim et al, 2007;Hoshino et al, 2008). Thus, Alstroemeria will continue to be used in future studies to investigate the role of CYC in the expression of floral zygomorphy.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, molecular mechanisms controlling flower development in this plant species have been studied by analysing class B genes (Hirai et al, 2007) and LEAFY-like gene (Hirai et al, 2012). Additionally, genetic transformation of Alstroemeria has been established by using Agrobacterium tumefaciens (Kim et al, 2007;Hoshino et al, 2008). These research findings will be useful for further analyses of flower development in this plant species.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of CYCLOIDEA-like genes in controlling floral zygomorphy in the monocotyledon Alstroemeria

Hoshino

Igarashi

Ohshima

et al. 2014

Scientia Horticulturae

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The CYCLOIDEA (CYC) gene controls the development of zygomorphic flowers and the determination of adaxial identity of floral organs in the model developmental system of Antirrhinum majus. However, whether CYC homologue genes also control floral zygomorphy in monocotyledon Alstroemeria plants is yet unknown. In this study, we investigated CYC-like genes in the monocotyledons Alstroemeria aurea, A. magenta, and A. pelegrina var. rosea, all of which have zygomorphic flowers. Since the CYC gene belongs to the T-complex protein (TCP) gene family of transcription factors, cloning of CYC-like sequences was performed using rapid amplification of cDNA ends (RACE)-polymerase chain reaction (PCR) by using degenerate primers designed for the TCP domain. We cloned 1 CYC-like sequence each from A. aurea (AaTCP1, accession number AB714967 in the GenBank/EMBL/DDBJ databases) and A. magenta (AmTCP1, AB714970), and 2 CYC-like sequences from A. pelegrina var. rosea (ApTCP1, AB714968; and ApTCP2, AB714969). The deduced amino acid sequences of AaTCP1, AmTCP1, ApTCP1, and ApTCP2 shared 67.7%, 67.7%, 71.0%, and 64.5% identities, respectively, with the TCP domain in CYC. Molecular phylogenetic analysis indicated that 3 CYC-like genes from Alstroemeria belonged to the ZinTBL1b clade in the CYC-/tb1-like subfamily. Reverse transcription (RT)-PCR and in situ hybridization analyses showed that AaTCP1 transcripts were specifically detected in flower buds and localized in the base of adaxial inner perianth of A. aurea. These results suggest that CYC-like genes are also involved in the development of floral asymmetry and the determination of adaxial identity of floral organs in the monocotyledon Alstroemeria.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of CYCLOIDEA-like genes in controlling floral zygomorphy in the monocotyledon Alstroemeria

Hoshino

Igarashi

Ohshima

et al. 2014

Scientia Horticulturae

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“…Typically, embryo rescue and ovule culture have been effectively used to overcome genetic incompatibility and develop interspecific hybrids in several crops (Morgan, 2004;Kaushal et al, 2005;Eeckhaut et al, 2007;Vlachostergios et al, 2007;Hoshino et al, 2008). Nevertheless, both techniques are not very useful when pro-embryos are used, as they can be damaged during excision due to their very small size.…”

Section: Introductionmentioning

confidence: 99%

Interspecific hybridization between Tigridia pavonia and T. augusta through ovary slice culture

Piña-Escutia¹,

Vázquez-García²,

Fernández³

2013

Genet. Mol. Res.

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Tigridia pavonia is the most popular species in the Tigridia genus, and is currently marketed in Europe, Asia, and Australia as a landscape plant. Although it is native to Mexico, there are no breeding programs for it. In this study, we attempted to increase its flower color spectrum and growth habit by interspecific hybridization with T. augusta. Interspecific hybrids between T. pavonia and T. augusta were successfully obtained for the first time using the cut-style pollination and ovary slice culture techniques. On the contrary, no hybrids were obtained from a reciprocal cross. At three, four, and five days after pollination (DAP) ovaries were sliced and cultured on Murashige and Skoog medium without growth regulators and ammonium nitrate, but were supplemented with 6% sucrose, 50 mg/L yeast extract, and 0.25% Gelrite. After 80 days of culture initiation, the germination of only 10 embryos was observed in ovary slices cultured at three DAP. After transfer to identical fresh medium, six hybrid embryos developed into seedlings. All obtained hybrid seedlings were transplanted successfully to soil, and grew normally. The progenies investigated were identified as true hybrids based on randomly amplified polymorphic DNA analysis.

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“…한편 유전자총을 이용한 형질전환 나리식물체 개발에 관한 연구에서는 1998년 최초로 Watad et al (1998) (Hoshino et al 2008;Kim et al 2007;Lin et al 2000), 히야신스 (Popowich et al 2007), 심비디움 (Roh et al 2011 and2013), 팔레놉시스 (Roh et al 2014)나 튤립 (Wilmink et al 1992) 등과 비교하면 형질전환연 구가 비교적 많이 수행되었음을 알 수 있다. 나리 형질전환 연구에 많이 사용된 아그로박테리움 방법은 비교적 안정성 과 재현성이 높고, 형질전환 시 유전자 copy수가 낮게 들어 가는 장점이 있지만 (Hiei et al 1997), 숙주식물에 제한이 있 고 박테리아 배양 및 공동배양에 시일이 오래 걸리는 단점 도 있다 (Hiei et al 1994).…”

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Optimization of a protocol for the production of transgenic lily plants via particle bombardment

Kim

2017

J Plant Biotechnol

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Transgenic lily plants have been obtained after particle bombardment, using PDS-1000/He system and scale explants of lilies, followed by PPT (D-L-phosphinothricin) selection. In this study, scales of the lily plants cv. 'red flame' were bombarded with a plasmid containing the bar gene as a selectable marker, and the AtSIZ gene as a gene of interest, showing salt tolerance and drought tolerance respectively, and both being driven by the CaMV 35S promoter. For optimization of a protocol, factors which optimized and showed a high transformation efficiency under following conditions, were considered: a bombardment pressure of 1100 psi, a target distance of 6 cm and 1.0 µm of gold particle, and 24-h pre-culture and post-culture on MS medium containing 0.2 M sorbitol and 0.2 M mannitol as osmoticum agents. After bombardment, all the bombarded scales of lily were transferred to MS medium without selective agents, for a week. Subsequently, these bombarded scales were transferred to a selection MS medium containing 10 mg/l PPT, and incubated for a month for further selection, after which they were cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. After transferring into hormone-free MS medium, the PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets. PCR analysis revealed that the surviving putatively transformed plantlets indicated the presence of both the bar gene and the AtSIZ gene. In conclusion, when 100 scales of lily cv. Red flame are bombarded, this study produced approximately 17-18 transgenic plantlets with an optimized bombardment protocol. The protocol described here can contribute to the breeding program of lilies.

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Plant regeneration from suspension cells induced from hypocotyls derived from interspecific cross Alstroemeria pelegrina × A. magenta and transformation with Agrobacterium tumefaciens

Cited by 4 publications

References 23 publications

Characterization of CYCLOIDEA-like genes in controlling floral zygomorphy in the monocotyledon Alstroemeria

Characterization of CYCLOIDEA-like genes in controlling floral zygomorphy in the monocotyledon Alstroemeria

Interspecific hybridization between Tigridia pavonia and T. augusta through ovary slice culture

Optimization of a protocol for the production of transgenic lily plants via particle bombardment

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