Plant regeneration in eggplant (Solanum melongena L.): A review

Sidhu, M.; Dhatt, Ajmer Singh; Sidhu, G. S.

doi:10.5897/ajbx2013.13521

Cited by 8 publications

(4 citation statements)

References 37 publications

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“…Genotype is the most important factor influencing somatic embryogenesis, with significant quantitative differences in their ability to form embroids in S. melongena,S. nigrum and S. torvum (Huda et al, 2007;Mir et al, 2008;Mir et al, 2011;Sidhu et al, 2014;Sharada et al, 2019;Ghan Singh et al, 2021a).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

Maloth¹,

Marka

Nanna

2023

EJBIO

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In this review, we report on the different plant growth regulators (PGRs) affecting the callus induction, callus-mediated regeneration, organogenesis, somatic embryogenesis from different explants and micropropagation through shoot tip and nodal cultures in Turkey Berry (Solanum torvumSW). The fruits of this plant guard against liver and kidney damage, stop certain cancers, and regulate blood sugar levels. They benefit digestion, the management of gout and menstruation, the treatment of anaemia and diabetes, the prevention of intestinal worms, cardiovascular disorders, and strokes. The protocol developed for the induction of callus can be utilized to isolate pharmaceutically important secondary metabolites in S. torvum, and the regeneration protocols optimized in this study can be used for genetic transformation and conservation of S. torvum, not only as a medicinal plant but also a model system.

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Section: Somatic Embryogenesismentioning

confidence: 99%

A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

Maloth¹,

Marka

Nanna

2023

EJBIO

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“…It is believed that Solanum macrocarpon (Gboma eggplant) and Solanum melongena L. are two more related relatives [7,8]. It can be grown in both tropical and subtropical regions and is a very prolific crop with a wide range of adaptability [9,4].…”

Section: Introductionmentioning

confidence: 99%

A review on post-harvest technology of an underutilized vegetable bitter tomato (Solanum aethiopicum L cv. gilo) of Northeastern region of India

Jamir,

Devi,

Ningombam

et al. 2024

BIO Web Conf.

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Northeastern region of India is endowed with variety of local vegetables that are highly nutritious and health-promoting. One of which is Solanum aethiopicum L which has a bountiful source of nutrients and bioactive chemicals. It has several pharmacological benefits and are used in indigenous medicine to treat various conditions. Nonetheless, the crop is highly perishable with a short shelf life (35days) which significantly contributes to postharvest losses. Techniques used to extend the shelf life of the crop are freezing, drying and refrigeration. The crop can be stored at low temperatures about 10-12oC. Fruits kept in perforated polyethylene bags had the longest shelf life. Important materials include polyethylene, polypropylene, and polystyrene are commonly used and coating can be applied to increase the crop’s shelf life. The crop has a huge potential in making value added products like pickles, dehydrated products and many more. However, concerns related to postharvest and production of the crop have not received equal attention due to scant research with the crop being neglected and underutilized due to lack of awareness and limited market. Considering the crop offers many advantages, popularization of the crop is essential but adapting to the bitter taste and flavor of the fruit could be challenging for the consumers. Consumer attitudes, views, and willingness to pay for products that have undergone particular post-harvest procedures require further investigation. Proper cultivation techniques, processing, value addition and marketing are required to surpass these hurdles.

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“…However, most investigations used genotypes from India or Europe. Genotype is one of the most important factors for determining the capacity of plant tissue to form somatic embryos and then to regenerate into a whole plant (Sidhu et al 2014). This study reports a simple and efficient procedure for the Agrobacterium-mediated transformation and regeneration of a Philippine eggplant cultivar 'PH 11424' with A. tumefaciens harboring a binary vector that confers a Cas9 expression cassette and resistance to the antibiotic hygromycin.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated Genetic Transformation and Plant Regeneration from Cotyledons in Philippine Eggplant (Solanum melongena L.) Acc. ‘PH 11424’

Sagarbarria,

Caraan,

Lipio

et al. 2024

Philipp Agric Scientist

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Eggplant (Solanum melongena L.) is one of the most important vegetables grown and consumed in the Philippines hence, continuous breeding programs are vital to maintain the supply of this economically important crop. This study demonstrates the first successful Agrobacterium-mediated genetic transformation and plant regeneration of the Philippine eggplant cultivar 'PH 11424', also known as 'Mistisa'. Cotyledons from 2-wk-old seedlings were used as explants, which were transformed with disarmed Agrobacterium tumefaciens strain LBA4404 harboring a binary vector for CRISPR/Cas9 expression and hygromycin phosphotransferase (HPT), an antibiotic selection marker. Growth of shoot primordia from the agro-infected explants was observed during selective culture with 7.5 ppm hygromycin, which indicated an initial success in transformation. The putatively transformed shoot primordia were then transferred to an elongation medium. The elongated and hygromycin-resistant shoots were allowed to develop roots in hormone-free medium supplemented with hygromycin, and subsequently acclimatized under greenhouse conditions. The entire process took at least 5 -6 mo. Of the total 585 agro-infected explants, the regeneration efficiency of rooted shoots was 4.6%. Successful transformation was confirmed by polymerase chain reaction (PCR) amplification of Cas9. Acclimatized plants tested positive for both transgenes. The surviving transgenic eggplants successfully reached maturity, flowered and set seed. These results demonstrate a working Agrobacterium-mediated transformation and plant regeneration protocols using cotyledons as explants in a Philippine eggplant genotype. These biotechnology tools are critical for the successful application of genetic engineering (GE) and new breeding techniques (NBTs) in eggplant crop improvement.

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Plant regeneration in eggplant (Solanum melongena L.): A review

Cited by 8 publications

References 37 publications

A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

A review on post-harvest technology of an underutilized vegetable bitter tomato (Solanum aethiopicum L cv. gilo) of Northeastern region of India

Agrobacterium-mediated Genetic Transformation and Plant Regeneration from Cotyledons in Philippine Eggplant (Solanum melongena L.) Acc. ‘PH 11424’

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