Plant regeneration of Carica papaya L. through somatic embryogenesis in response to light quality, gelling agent and phloridzin

Ascencio-Cabral, Azucena; Pulido, Humberto Gutiérrez; Rodrı́guez-Garay, Benjamı́n; Gutiérrez-Mora, Antonia

doi:10.1016/j.scienta.2008.06.014

Cited by 48 publications

(29 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several studies in the literature have described the use of the auxin 2,4-D to induce embryogenesis in papaya explants (Castillo et al 1998, Fernando et al 2001, Renukdas et al 2003, Yu et al 2003, Ascencio-Cabral et al 2008, Malabadi et al 2011). The capacity of 2,4-D to activate embryogenic routes may be related to its ability to induce stress genes that contribute to the cellular reprogramming of somatic to embryogenic cells (Fehér 2005(Fehér , 2015.…”

Section: Induction Of Somatic Embryogenesismentioning

confidence: 99%

“…Commercially, papaya trees are propagated from seeds; however, seeds can be used for other purposes. For example, zygotic embryos (Ascencio-Cabral et al 2008, Farzana et al 2008, Malabadi et al 2011, Anandan et al 2012 or segments of seedlings (Yu et al 2003, Saha et al 2004, Farzana et al 2008, Homhuan et al 2008 can be used in tissue culture to produce explants and somatic embryos via somatic embryogenesis. This technique produces large numbers of somatic embryos that can be transformed into plantlets, identified as hermaphrodite ex vitro and subsequently rescued in vitro and used in the field.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heartshape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L -1 ) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus -1 ) and the lowest percentage of early germination (4%).

show abstract

Section: Induction Of Somatic Embryogenesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…Fitch and Manshardt (1990) established papaya embryogenic cultures from immature zygotic embryos inoculated in MS medium with half the saline concentration, supplemented with 0.45-113.1 µM 2,4-dichlorophenoxyacetic acid (2,4-D) for three weeks. As a result of this study, the addition of auxin in the culture medium of immature zygotic embryos became commonly used for the induction of somatic embryogenesis of C. papaya (Bhattacharya et al 2003, Farzana et al 2008, Ascencio-Cabral et al 2008, Malabadi et al 2011, Anandan et al 2012, including both genitors of the UENF/CALIMAN 01 hybrid, Solo (Malabadi el al. 2011) and Formosa (Almeida et al 2001).…”

Section: Introductionmentioning

confidence: 97%

Polyethylene glycol effects on somatic embryogenesis of papaya hybrid UENF/CALIMAN 01 seeds

Heringer¹,

Vale²,

Barroso³

et al. 2013

Theor. Exp. Plant Physiol.

View full text Add to dashboard Cite

Among commercial fruits, papaya (Carica papaya L.) is notably one of the most common ones around the world. The aim of the current study was to establish a somatic embryogenesis protocol for the hybrid UENF/CALIMAN 01, elucidating the effects of ). The 60 g L -1 PEG treatment group had the highest average for the number, maturation and conversion of somatic embryos that resulted in plantlets at the end of the process. The addition of PEG to MS medium led to increased protein synthesis. These results are unprecedented for C. papaya hybrid UENF/CALIMAN 01 as the current study demonstrates the development of somatic embryogenesis in this high quality hybrid.

show abstract

“…2, Figs 1B-G), indicating the ease and responsiveness of papaya to in vitro culture, but the quantitative and qualitative outcomes differed. SFC and HC were best (or most) induced from the Fitch (1993) and Ascêncio-Cabral et al (2008) methods, respectively, for both cultivars, based on a score of 3, with significantly higher fresh weight and significantly higher percentage survival (relative to all other protocols) (Tab. 2).…”

Section: Resultsmentioning

confidence: 99%

“…This is the first such protocol for papaya and can serve to preserve important germplasm using LYS or cryopreservation (Teixeira da ). Using two cultivars, 'Rainbow' and 'Sunrise Solo', optimal soft, white and friable callus was obtained using the Fitch (1993) protocol while harder brown callus was obtained using the Ascêncio-Cabral et al (2008) protocol. Most likely, if tweaked, the callus-induction protocol could also serve for other cultivars or even for other explant types, such as stem (node or internode) tissue.…”

Section: Discussionmentioning

confidence: 99%

Callus induction in papaya (Carica papaya L.) and synseed production for low temperature storage and cryopreservation

Silva

2014

Folia Horticulturae

View full text Add to dashboard Cite

The mid-to long-term preservation of papaya (Carica papaya L.) would allow for the safeguarding of important germplasm. In this study, soft friable callus (SFC) and hard callus (HC) were induced from the first two true leaves of 10-day-old seedlings containing a midrib derived from the germinated seed of two cultivars ('Rainbow' and 'Sunrise Solo'). Following germination on a Murashige and Skoog (MS) medium that contained 3% sucrose and was free of plant growth regulators (PGRs), sections of the first true leaves from 10-day-old seedlings were exposed to seven published callus or somatic embryogenesis protocols for zygotic embryos, leaves or hypocotyls. Optimal SFC and HC induction was carried out on a half-strength MS medium following the Fitch (1993) or the Ascêncio-Cabral et al. (2008) protocol, respectively. SFC formed shoots that could then convert to plants when transferred to a full-strength MS medium devoid of PGRs. Plantlets 10-cm tall were acclimatised in two steps: first by in vitro acclimatisation in aerated vessels, the Vitron, under CO 2 -enriched (3000 ppm CO 2 ), then by the transfer of individually rooted plantlets in Rockwool ® blocks to a substrate of soil: pine bark : perlite (1:1:1, v/v/v). SFC and HC were then encapsulated in alginate beads, which were exposed to low temperature storage (LTS) at 10°C and 15°C, and also cryopreserved for 30 days. All encapsulated alginate beads that contained SFC, HC or leaf tissue that had been stored under LTS or cryopreserved were able to regenerate callus when placed on an optimal callus induction medium. Plants derived from the control, LTS and cryopreservation protocols, either from SFC or HC, were successfully acclimatised.

show abstract

Plant regeneration of Carica papaya L. through somatic embryogenesis in response to light quality, gelling agent and phloridzin

Cited by 48 publications

References 23 publications

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Polyethylene glycol effects on somatic embryogenesis of papaya hybrid UENF/CALIMAN 01 seeds

Callus induction in papaya (Carica papaya L.) and synseed production for low temperature storage and cryopreservation

Contact Info

Product

Resources

About