Plant regeneration of sweetpotato (Ipomoea batatas L.) from leaf explants in vitro using a two-stage protocol

Dessai, Ananta Porobo; Gosukonda, Ramana; Blay, Essie; Dumenyo, C. Korsi; Medina-Bolívar, Fabricio; Prakash, Channapatna S.

doi:10.1016/0304-4238(95)00767-n

Cited by 14 publications

(8 citation statements)

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“…Dessai et al (1995) first demonstrated the use of the two-stage approach by first exposing Fig. 3 Adventitious shoot regeneration in sweetpotato genotypes under a two stages 'auxin-cytokinin' regime.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro shoot regeneration studies Leaves (lamina with petioles of 0.5-1.5 cm) from the first third of 4-6 week-old in vitro grown plants were used as explants, as recommended by Dessai et al (1995). The regeneration medium contained MS with minimal organics (Sigma-Aldrich), sucrose (30 g l -1 ), Phytagel (3 g l -1 ), with pH adjusted to 5.7.…”

Section: In Vitro Culture Initiationmentioning

confidence: 99%

“…For instance, Dessai et al (1995) found significant differences in the regeneration frequency among 27 genotypes from a wide geographical distribution when they were put under a 2,4-D (0.2 mg l -1 ) and zeatin (0.2 mg l -1 ) regime to induce shoot organogenesis. Among these 27 genotypes, eighteen exhibited shoot regeneration with frequencies ranging from 10% to 83.3% and nine were recalcitrant to regeneration.…”

Section: Introductionmentioning

confidence: 98%

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Rapid shoot regeneration in industrial ‘high starch’ sweetpotato (Ipomoea batatas L.) genotypes

Santa-María

Pecota

Yencho

et al. 2009

Plant Cell Tiss Organ Cult

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Culture Initiationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Rapid shoot regeneration in industrial ‘high starch’ sweetpotato (Ipomoea batatas L.) genotypes

Santa-María

Pecota

Yencho

et al. 2009

Plant Cell Tiss Organ Cult

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“…The leaf explants (with lateral bud) were excised aseptically from 4/5-week-old in vitro cultures, as described by Dessai et al (1995), and cultured onto a shoot/protuberance induction medium. In experiment 1, leaf explants were cultured onto an MMS medium alone and also MMS supplemented with TDZ at various levels (0.5, 1.0, 2.0, 5.0, 10.0 or 20.0 μM).…”

Section: Plant Material Culture Media and Culture Conditionsmentioning

confidence: 99%

“…However, the lack of an efficient regeneration system is the major limiting factor which prevents the development of gene transfer technologies for commercially important cultivars of sweetpotato. There have been a number of proposals for the in vitro propagation of sweetpotato, including via adventitious shoot induction Dessai et al, 1995;Sihachakr et al, 1997;Santa-Maria et al, 2009) and somatic embryogenesis (Liu and Cantliffe, 1984;Chée et al, 1990;Desamero et al, 1994;Bieniek et al, 1995;Zheng et al, 1996;Dhir et al, 1998;He et al, 2009). Genetic transformation of sweetpotato, using various explants, has been reported, but with low efficiency (Newell et al, 1995;Gama et al, 1996;Morán et al, 1998;Okada et al, 2001;Berberich et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

Kumar¹,

Ku²,

Agrawal³

et al. 2013

bta

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In this report, a novel, reproducible and efficient protocol for protuberance-mediated adventitious shoot regeneration has been established for sweetpotato [Ipomoea batatas (L.) Lam.]. The leaf explants were cultured on a modified Murashige and Skoog (MS) medium containing various concentrations (0.5, 1.0, 2.0, 5.0 or 10 μM) of Thidiazuron (TDZ). The adventitious shoots were developed from the base of leaf explants on an MS medium supplemented with 2.0, 5.0 or 10.0 μM TDZ. The responsive explants were transferred to a freshly modified MS medium containing TDZ. Green-colored, oval, or cone-shaped shoot-forming protuberances were developed from explants. It appeared that the frequency of protuberance development depended on the concentration of TDZ. However, this did not further improve, when the concentration of TDZ increased from 5.0 μM to 10.0 μM. Adventitious shoot buds were developed from protuberances, and the histology of protuberances revealed a direct origin of shoot buds. In vitro raised shoots were rooted either on a modified MS medium alone or on a modified MS medium containing indole-3-butyric acid (IBA). The regenerated plants were acclimatized and successfully established in a greenhouse.

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Agrobacterium-Mediated Transformation in the Evolution of Plants

Matveeva

2018

Current Topics in Microbiology and Immunology

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In most cases, the genetic engineering of plants uses Agrobacterium-mediated transformation to introduce novel genes. In nature, insertion of T-DNA into the plant genome and its subsequent transfer via sexual reproduction have been shown for several species in the genera Nicotiana, Ipomoea , and Linaria . A sequence homologous to T-DNA of the Ri plasmid of Agrobacterium rhizogenes was found in the genome of wild-type Nicotiana glauca (section Noctiflorae) more than 30 years ago and was named "cellular T-DNA" (cT-DNA). It comprises an imperfect inverted repeat and contains homologs of several T-DNA oncogenes (NgrolB, NgrolC, Ngorf13, Ngorf14) and an opine synthesis gene (Ngmis). Multiple cT-DNAs have also been found in species of the sections Tomentosae and Nicotiana of the genus Nicotiana. These ancient cT-DNA genes are still expressed, indicating that they may play a role in the evolution of these plants. In 2012-2013, cT-DNA was detected and characterized in Linaria vulgaris and L. genistifolia ssp. dalmatica. Their cT-DNA is present in two copies and organized as an imperfect direct tandem repeat, containing LvORF2, LvORF3, LvORF8, LvrolA, LvrolB, LvrolC, LvORF13, LvORF14, and the Lvmis genes. In 2015, cT-DNA was found in Ipomoea. Two types of T-DNA-like sequences were described within this genera, and their distribution varied among cultured hexaploid, tetraploid, and wild diploid forms. Thus, several independent T-DNA integration events occurred in the genomes of these three plant genera. We propose that the events of T-DNA insertion in the plant genome might have affected their evolution, resulting in the creation of new plant species. In this chapter, we focus on the structure and functions of cT-DNA in Linaria, Nicotiana, and Ipomoea and discuss their possible evolutionary role.

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Plant regeneration of sweetpotato (Ipomoea batatas L.) from leaf explants in vitro using a two-stage protocol

Cited by 14 publications

References 4 publications

Rapid shoot regeneration in industrial ‘high starch’ sweetpotato (Ipomoea batatas L.) genotypes

Rapid shoot regeneration in industrial ‘high starch’ sweetpotato (Ipomoea batatas L.) genotypes

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

Agrobacterium-Mediated Transformation in the Evolution of Plants

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