Plant regeneration through callus cultures derived from immature-cotyledon explants of oleaster (Elaeagnus angustifolia L.)

Karami, Omid; Piri, Khosro; Bahmani, Reza

doi:10.1007/s00468-008-0281-0

Cited by 9 publications

(5 citation statements)

References 11 publications

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“…Table 3 lists the overall in vitro experiments in H. rhamnoides and H. salicifolia. Similar results were observed for multiple shooting from BAP supplemented medium in berry bearing plant, Elaeagnus angustifolia [49,50] . The species of Elaeagnaceae family or the oleaster are majorly propagated vegetatively (hardwood cutting), while only few were studied for their in vitro shootings.…”

Section: Shoot Organogenesissupporting

confidence: 84%

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A review on genetic diversity, micropropagation and transformations in the high-value medicinal plant of Himalayas-Seabuckthorn (Hippophae sp.)

Balasubramaniam,

Ganeshan,

Natesan

et al. 2024

J Pharmacogn Phytochem

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Seabuckthorn (Hippophae sp.) is popularly known as the "Gold Rush" for its commercial value. It holds well-established vegetative propagation and in vitro regeneration techniques. Accordingly, micropropagation of Hippophae sp. from multiple explants follows the developmental stages of establishment, shoot proliferation, and rooting. The successful in vitro propagation protocols utilized Murashige and Skoog medium and Woody Plant Medium supplemented with cytokinin for shoot organogenesis, somatic embryogenesis, and auxins for high rooting frequency. Subsequent acclimatization of Hippophae sp. with a reasonable survival rate was recorded under greenhouse conditions. The genetic transformations in seabuckthorn are yet to be studied in detail. Still, initial studies were performed with conventional methods using Agrobacterium rhizogenes (indirect) and biolistics (direct), which could support extensive transformation research in the future for commercialization and conservation. This short review provides an overview of compiled works on the tissue culture of Hippophae rhamnoides and Hippophae salicifolia that could assist in further assessment and vast applications.

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Section: Shoot Organogenesissupporting

confidence: 84%

“…3. From the literature search the species Elaeagnus angustifolia has shown formation of lateral roots in IBA which can be the recommended root induction regulator for this family [49] .…”

Section: Multiplication and Rootingmentioning

confidence: 99%

A review on genetic diversity, micropropagation and transformations in the high-value medicinal plant of Himalayas-Seabuckthorn (Hippophae sp.)

Balasubramaniam,

Ganeshan,

Natesan

et al. 2024

J Pharmacogn Phytochem

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“…Also, BAP produced higher number of shoots than other cytokinins. According to other studies, Karami et al (2009) andMurthy et al (2010) reported that BAP exerts a powerful influence on shoot multiplication in several cases. Jaiswal and Narayan (1985) reported that shoots regenerated via stem segments of adult plants of F. religiosa callus cultured on the MS medium with 1.0 mg · L -1 BA.…”

Section: Resultsmentioning

confidence: 95%

“…In our experiments, the high frequency of regeneration was obtained from type I calli in MS medium containing 1.5 mg · L -1 BA in combination with 0.15 mg · L -1 IBA. Preliminary experiments indicated that the texture of the callus is an important factor in organogenesis response (Chen et al, 2016;Karami et al, 2009). In accordance with our results, Karami et al (2009) demonstrated that different regeneration responses through various callus types from cotyledon segments of E. angustifolia were obtained, according to the growth regulators.…”

Section: Resultsmentioning

confidence: 99%

“…Preliminary experiments indicated that the texture of the callus is an important factor in organogenesis response (Chen et al, 2016;Karami et al, 2009). In accordance with our results, Karami et al (2009) demonstrated that different regeneration responses through various callus types from cotyledon segments of E. angustifolia were obtained, according to the growth regulators. Chen et al (2016) investigated the different growth regulators and obtained various regeneration frequencies via different callus types from leaf explants of Chirita swinglei.…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Adventitious Shoot Regeneration through Direct and Indirect Organogenesis from Seedling-derived Hypocotyl Segments of Ficus religiosa L.: An Important Medicinal Plant

Hesami

Daneshvar

2018

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Ficus religiosa is an important industrial, medicinal, and ornamental plant, so in vitro regeneration is of high paramount in this valuable germplasm. Two efficient protocols were developed for indirect and direct shoot organogenesis through hypocotyl explants. In the first experiment, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and indole butyric acid (IBA) (0.5, 1.0, and 1.5 mg·L−1) in combination with 6-benzyl amino purine (BAP) (ratio 10:1, respectively) were used for callus formation. Two types of callus were obtained from different concentrations of plant growth regulators (PGRs). Also, 2,4-D produced yellow-brownish and friable callus (Type I), whereas the green and compact callus (Type II) was achieved in IBA. The highest callus fresh weight (2.43 g) was observed in Murashige and Skoog (MS) medium containing 0.5 mg·L−1 2,4-D plus 0.05 mg·L−1 BAP. In the later experiments, various concentrations of thidiazuron (TDZ), 6-furfuryl amino purine (KN), and BAP (0.25, 0.5, 1.0, and 1.5 mg·L−1) in combination with IBA (ratio 10:1, respectively) were applied for shoot regeneration (direct and indirect organogenesis). In shoot regeneration from callus, the highest regeneration frequency (86.66%) and shoot number per callus (4.13) were achieved in MS medium supplemented with 1.5 mg·L−1 BAP plus 0.15 mg·L−1 IBA from type I calli. However, no regeneration was observed in type II calli. In direct shoot regeneration, the highest regeneration frequency (96.66%) and shoot number (6.26) were obtained in the medium mentioned previously. In root induction experiment, different concentrations of naphthalene acetic acid (NAA) and IBA alone or in combination were applied, and MS medium containing 2.0 mg·L−1 IBA along with 0.1 mg·L−1 NAA was the best hormonal balance for root induction. The rooted plantlets’ survival rate was more than 95% in the acclimatization stage. These results demonstrated that the direct regeneration method provides more shoot regeneration frequency and take a less time for shoot organogenesis than the indirect regeneration method. Based on our knowledge, this study is the first report of direct and indirect shoot organogenesis of F. religiosa via hypocotyl from in vitro–grown seedling.

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In vitro plant regeneration from cotyledon-derived callus cultures of leguminous tree Gleditsia caspica Desf.

2014

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Plant regeneration through callus cultures derived from immature-cotyledon explants of oleaster (Elaeagnus angustifolia L.)

Cited by 9 publications

References 11 publications

A review on genetic diversity, micropropagation and transformations in the high-value medicinal plant of Himalayas-Seabuckthorn (Hippophae sp.)

A review on genetic diversity, micropropagation and transformations in the high-value medicinal plant of Himalayas-Seabuckthorn (Hippophae sp.)

In Vitro Adventitious Shoot Regeneration through Direct and Indirect Organogenesis from Seedling-derived Hypocotyl Segments of Ficus religiosa L.: An Important Medicinal Plant

In vitro plant regeneration from cotyledon-derived callus cultures of leguminous tree Gleditsia caspica Desf.

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