Plant regeneration through various explants of<i>Lilium longiflorum</i>hybrid “Bright Tower” and determination of ploidy level of regenerated plants

Naing, Aung Htay; Yun, Haesun; Lucidos, Juniel Galido; Hwang, Yoon Jung; Kim, C.K.; Ahn, Byung Joon; Lim, Ki-Byung

doi:10.1080/11263504.2012.754384

Cited by 12 publications

(5 citation statements)

References 22 publications

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“…Addition of plant growth regulator on medium tissue culture played an important role in triggering cell division and elongation so that it was able to accelerate the development and growth (Gunawan et al 1992). This is in line with the study by Naing et al (2014) reporting that culture leaf explants L. longiflorum Thunb. after 15 days of culture showed elongation and enlargement.…”

Section: Initiation Callussupporting

confidence: 92%

Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP

et al. 2019

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Lestari NKD, Deswiniyanti NW, Astarini IA, Arpiwi NLM. 2019. Callus and shoot induction of leaf culture Lilium longiflorum with . Lilium longiflorum Thunb., an Easter lily, is a common ornamental plant used as potted plant, cut flower, cosmetic and medicine. The research on tissue culture technique to induce shoots and callus is expected to increase the yield of lilies quickly and efficiently. This study aims to determine the effect of plant growth regulator combi nation of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP) on the development of leaf culture and the best concentration for callus induction and shoots of lily plants. This study was conducted in a completely randomized design using leaf explants in Vacin and Went medium, with combination of NAA and BAP (0; 0.5; 1 mg..L -1 ) as the treatments. The result of the eight-week observations shows that the plant growth regulators significantly affected either in days initiation callus, days initiation of shoot, percentage of callus, percentage of shoot, diameter of callus, no of shoot and length of shoot. The combination of 1 mg.L -1 NAA and BAP had the best effect in the percentage of explants forming shoots (100%), means number of shoots (5.8), and means length of shoot (11.6 cm).

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Section: Initiation Callussupporting

confidence: 92%

Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP

et al. 2019

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“…ese studies show that while all cells in a plant have the potential to regenerate the plant, the degree of difficulty in expressing totipotency varies between different lily varieties and even between different cells within the same plant [29]. Similarly, Akçal and Kahraman [28] and Naing et al [30] stated that the success of scale regeneration and subsequent scale formation depends largely on the explant and the presence of growth regulators. Deswiniyanti and Dwipayani-Lestari [16] emphasized the importance of endogenous phytohormone content and the balance of bulb scales to regenerate new vegetative structures.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Bulbification of Five Lily Varieties: An Effective Method to Produce Quality Seeds and Flowers

Lapiz-Culqui

Meléndez-Mori

Mállap-Detquizán

et al. 2022

International Journal of Agronomy

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Lilies are one of the most important, beautiful, and economically valuable flowers in the world. Lilium is regarded as a popular floral trade cut flower, so viable protocols are needed to provide seed production, multiplication, and preservation. In vitro protocols allow for rapid large-scale production and rejuvenation of planting material, but to be a commercially viable multiplication method, the procedure must allow for rapid production of viable, true-to-type plants quickly. The objective was to evaluate the in vitro production of microbulbs of five lily varieties (Lilium “Champion Diamond,” Lilium “Yellow Diamond,” Lilium “Batavus,” Lilium “Hyde Park,” and Lilium sp.) using different concentrations of 6-benzylaminopurine (0, 0.5, 1.0, 1.5, 1.5, and 2.0 mg L−1 BAP) and to determine the commercial quality (flowering) of the plants grown from the seed obtained. Results from the micropropagation phase show Lilium “Batavus” and Lilium “Hyde Park” varieties had better in vitro responses, especially when grown with 1.0 and 1.5 mg L−1 BAP, respectively. Plants (of all varieties) grown from microbulbs showed positive growth and generally resulted in commercially viable flower production. Finally, the results of this study support the use of bulb scales as an alternative for obtaining vegetative seeds with high potential for lily cultivation.

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“…Regeneration of plants through plant tissue culture is the best technique to produce true-to-type plants ( Ali et al., 2017 ). However, the genetic stability of in vitro -grown plants can be altered by mutation and error in cell division, which results in the formation of genetically and genomically variant cells ( Naing et al., 2014 ). Moreover, genetic alterations may be affected by the age of the culture ( Samarina et al., 2019 ), the composition of the culture medium, and specifically the different combinations and concentrations of plant growth regulators ( Gantait and Vahedi, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced wedelolactone content in in vitro-raised genetically uniform Wedelia chinensis under the influence of CuSO4

Swami,

Nimker,

Narula

et al. 2023

Front. Plant Sci.

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In the present study, we addressed the imperative for potent anticancer agents through Wedelia chinensis, a medicinal plant abundant in the robust antihepatotoxic and antitumor compound wedelolactone. Hindrances in conventional propagation methods due to cross-pollination and habitat degradation prompted us to pioneer in vitro rapid multiplication using plant tissue culture. Optimal outcomes were attained employing Murashige and Skoog (MS) medium supplemented with Indole-3-butyric acid (IBA) (0.5 mg/L) and Kinetin (KN) (5.0 mg/L), yielding 97.67% shoot regeneration and 81.67% rooting from nodal explants. Transplanted plantlets exhibited a 92% survival rate. We established a wedelolactone extraction protocol using toluene:ethyl acetate:formic acid (5:4:1) for High-performance thin-layer chromatography (HPTLC) analysis, trailblazing wedelolactone quantification and 2C DNA analysis in W. chinensis via flow cytometry. Experiments under heavy metal stress with CuSO4 unveiled physiological responses, with peak wedelolactone content [193.90 μg/g dry weight (dw)] in vitro at 75 μM CuSO4, surpassing in vivo levels (89.95 μg/g dw) by 116%. By pioneering successful in vitro rapid multiplication and enhanced wedelolactone content, we bridge a critical gap in the conservation and production of this medicinal plant. Our findings not only offer a sustainable means of propagation but also present a viable strategy for elevating the yield of potent bioactive molecules like wedelolactone, holding immense promise for the development of novel therapeutic interventions and addressing the pressing healthcare challenges of our time.

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Plant regeneration through various explants ofLilium longiflorumhybrid “Bright Tower” and determination of ploidy level of regenerated plants

Cited by 12 publications

References 22 publications

Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP

Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP

In Vitro Bulbification of Five Lily Varieties: An Effective Method to Produce Quality Seeds and Flowers

Enhanced wedelolactone content in in vitro-raised genetically uniform Wedelia chinensis under the influence of CuSO4

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