Plant regeneration via indirect somatic embryogenesis and optimisation of genetic transformation in Coffea arabica L. cvs. Caturra and Catuaí

Gatica-Arias, Andrés; Arrieta-Espinoza, Griselda; Esquivel, Ana Mercedes Espinoza

doi:10.2225/vol11-issue1-fulltext-9

Cited by 47 publications

(56 citation statements)

References 22 publications

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“…Morphological observation using a stereo microscope showed that pre-embryos were formed along the incision site of the leaf indicating a direct embryogenesis process of the leaf. The nonembryogenic calli were similar with that reported by Gatica et al (2007) who obtained low frequency of callus formation in inducing direct somatic embryogenesis of arabica coffee cv. Catura and Catuai (Gatica et al 2008).…”

Section: Embryogenic Callus Inductionsupporting

confidence: 89%

DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica)

Ibrahim¹,

Hartati

Rubiyo³

et al. 2013

Indones. J. Agric. Sci.

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Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryogenesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evaluated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µ M 2,4-D + 4.54 or 9.08 µ M thidiazuron; 4.52 µ M 2,4-D + 4.54 or 9.08 µ M thidiazuron; or 9.04 µ M 2,4-D + 9.08 µ M thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µ M and adenine sulfate 40 mg L -1 ). The results showed coffee leaf explant cultured on medium containing 2.26 µ M 2,4-D + 4.54 or 9.08 µ M thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µ M thidiazuron to induced indirect somatic embriogenesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µ M 2,4-D in combination with 9.08 µ M thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µ M 2,4-D + 9.08 µ M thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.

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Section: Embryogenic Callus Inductionsupporting

confidence: 89%

DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica)

Ibrahim¹,

Hartati

Rubiyo³

et al. 2013

Indones. J. Agric. Sci.

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“…Among these other methods are vacuum infiltration (Canché-Moor et al 2006), electroporation (Fernandez-Da Silva and Menéndez-Yuffá 2003) and biolistic or particle bombardment (Albuquerque et al 2009;Gatica-Arias et al 2008;Ribas et al 2005;Rosillo et al 2003;Van Boxtel et al 1995).…”

Section: Other Transformation Methodsmentioning

confidence: 99%

Somatic Embryogenesis in Coffea spp.

Loyola‐Vargas

Avilez-Montalvo

Aviles-Montalvo

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Coffee is a very important commodity in the world. It is a perennial plant and as a consequence its genetic improvement had been difficult. The biotechnology, in particular somatic embryogenesis (SE) has helped to the propagation of commercial coffee cultivars. Along almost 50 years, different research groups around the world have developed uncountable SE processes to produce an efficient, reproducible, and reliable SE process. Many of the factors that affect the embryogenic response have been studied. It has been demonstrated that the genetic modification of coffee is possible. This transformation can lead to the improvement of varieties with very attractive agronomic traits, such as low caffeine content, resistant to diseases and pests. IntroductionCoffee is one of the two major commodities in the world. The cultivation of coffee gives employment to more than 80 million people, and every year, more than 11 million hectares are cultivated around the world.All coffee species are trees or perennial woody bushes. The coffee plants differ among themselves in different aspects such as morphology, size, and ecological adaptation. Although there are more than 124 species of the Coffea genus, only two are used to produce coffee commercially, Coffea arabica and C. canephora, which accounts for 60 and 40 % of world coffee production, respectively. C. canephora (2n = 2x = 22) is an outcrossing diploid, allogamous, consisting of polymorphic populations of strongly heterozygous individuals and with high caffeine content. C. arabica is an allotetraploid (2n = 4x = 44) derived from a spontaneous

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“…EB penetrates membrane ruptures, turning cell interior blue early indicating cell deth (BHARGAVA et al, 2007). GATICA-ARIAS et al (2008) evaluated the embryogenic viability of cells suspensions of coffee plants using EB stain. The results showed that nonviable cells turned intensely blue, whereas viable cells did not react and showed isodiametric shape, dense cytoplasm, and nucleus with prominent nucleoli.…”

Section: Resultsmentioning

confidence: 99%