Plant regeneration via somatic embryogenesis from protoplasts of six plant species related to Citrus

Jumin, Hasan Basri; Nito, Nobumasa

doi:10.1007/s002990050027

Cited by 4 publications

(6 citation statements)

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“…Tal interpretação também foi feita por Fossard et al (1978) e Jarvis (1986 Ciênc. agrotec., Lavras, v. 29, n. 6, p. 1125Lavras, v. 29, n. 6, p. -1131 Entretanto, tais resultados discordam daqueles em que as concentrações de 0,1 mg L -1 (JUMIN & NITO, 1996;PASQUAL et al, 1990;SCHOOLER, 1960), 1 mg L -1 (CARIMI et al, 1998;KUNITAKE et al, 1991) ou 2 mg L -1 (GMITTER et al, 1990;ZDRUJKOVSKAJA-RICHTER, 1981) de GA 3 proporcionaram melhores resultados no cultivo in vitro de embriões.…”

Section: Resultsunclassified

Cultivo de embriões imaturos de citros em diferentes concentrações de carvão ativado e ácido giberélico

Chagas¹,

Pasqual²,

Ramos³

et al. 2005

Ciênc. agrotec.

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RESUMOAdição de carvão ativado e giberelina no meio de cultura podem proporcionar melhores condições no desenvolvimento de embriões imaturos de citros. Objetivou-se avaliar o efeito de carvão ativado e GA 3 (ácido giberélico) no cultivo de embriões imaturos provenientes do cruzamento entre laranjeira Pêra Rio x tangerineira Poncã . Após 118 dias da polinização, frutos imaturos, com 3 a 4 cm de diâmetro, foram coletados, suas sementes removidas e tratadas com álcool (70%) por cinco minutos, hipoclorito de sódio (2%) por 20 minutos e, posteriormente, lavadas três vezes em água destilada e autoclavada. Em condições assépticas, os tegumentos das sementes foram separados, os embriões globulares excisados e inoculados em tubos de ensaio contendo 15 mL do meio MT, acrescido de carvão ativado (0; 0,5; 1; 1,5 e 2 g L . Maior comprimento da parte aérea foi obtido em meio MT, acrescido de 0,1 e 1 mg L -1 de GA 3 , combinado com 2 g L -1 de carvão ativado. Maior comprimento do sistema radicular, massa da matéria fresca e número de folhas de plântulas foram obtidos em meio MT, acrescido de 0,01 mg L -1 de GA 3 , na ausência de carvão ativado. A adição de carvão ativado influenciou na concentração de ácido giberélico acrescido no meio de cultura. Termos para indexação:Citrus sinensis, Citrus reticulata, cultura de embriões. ABSTRACTActivated charcoal and gibberelin provides better conditions on development of citrus immature embryos. Activated charcoal and GA 3 (gibberelic acid) on Pêra Rio sweet orange x Poncã mandarin immature embryos culture was evaluated. After 118 dayspollination, imature fruits with 3 to 4 cm of diameter were collected, seeds removed and treated with alcohol (70%) for five min., sodium hypoclorite (2%) for 20 min. and three times washed with distilled and autoclaved water. In aseptic conditions, the teguments were separated, the globular embryos excised and inoculated in test tubes containing 15 mL of the MT medium culture, added of activated charcoal (0; 0.5; 1; 1.5 and 2 g L -1 ) and GA 3 (0; 0.01; 0.1; 1 and 10 mg L -1 ). After inoculation, the embryos were maintained for 90 days in growth room at 27±1ºC, 16 hours photoperiod and 32 mmol m -2 s -1 irradiance. Higher length of the aerial part was obtained in MT medium added 0.01 and 1 mg L -1 of GA 3 combined with 2 g L -1 of activated charcoal. Higher length of the roots system, fresh mass and number of leaves of seedlings was obtained in MT medium added 0.01 mg L -1 of GA 3 and activated charcoal absence. Addition of activated charcoal influenced in giberellic acid concentration added in MT medium.

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Section: Resultsunclassified

Cultivo de embriões imaturos de citros em diferentes concentrações de carvão ativado e ácido giberélico

Chagas¹,

Pasqual²,

Ramos³

et al. 2005

Ciênc. agrotec.

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“…Secondary embryogenesis is also observed (Ohgawara et al, 1985;Louzada et al, 1992), with pluricotyledonary embryos, multiple shoot meristems, fused embryos and fasciation (Jumin and Nito, 1996), but this does not interfere with germination and plant recovery. Embryo development is better in EME medium with 25 g of sucrose/l than with 50 g of sucrose/ l (Grosser and Gmitter Jr., 1990).…”

Section: Resultsmentioning

confidence: 93%

Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry

et al. 2000

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Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.

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“…However, adding 0.01 mg l -1 GA3 (Ribeiro et al 2000;Chagas et al 2003), 0.1 mg l -1 GA3 (Pasqual et al 1990;Jumin and Nito 1996) and 2 mg l -1 GA3 (Gmitter et al 1990) didn't give good results. This proves that there is not an ideal GA3 dose for species and varieties and the results may vary according to the species, varieties and embryo development stages.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, there were some results showing that high embryo germinations were obtained in adding 0.01 mg l -1 GA3 (Ribeiro et al 2000;Chagas et al 2003), 0.1 mg l -1 GA3 (Pasqual et al 1990;Jumin and Nito 1996) and 2 mg l -1 GA3 (Gmitter et al 1990) in culture medium. These situations may vary due to the embryo development stage, species and cultivars used.…”

Section: Discussionmentioning

confidence: 99%

“…However, GA3 concentrations in culture medium may give different results depending on the species or variety. Some authors observed that supplementing 0.01 mg l -1 GA3 to the medium (Ribeiro et al 2000;Chagas et al 2003), supplementing 0.1 mg l -1 GA3 to the medium (Pasqual et al 1990;Jumin and Nito 1996) and supplementing 2 mg l -1 GA3 (Gmitter et al 1990) favored the growth and development of embryos.…”

Section: Introductionmentioning

confidence: 99%

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Yerli mandarin x Carrizo sitranjı melezlerinde, embriyo kurtarma tekniğinde embriyo gelişim aşaması ve GA3 dozlarının optimizasyonu

Kurt

Ülger

2019

Mediterranean Agricultural Sciences

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Many difficulties are exist in citrus breeding due to incompatibility, long juvenility, sterility and nucellar embryo. Zygotic embryo in the seeds of polyembryonic citrus cultivar is degenerated by nucellar embryos during embryo development. Accordingly, in vitro embryo culture is a useful tool in citrus breeding, since it assures embryo germination and development. In this study, Common mandarin x Carrizo citrange were crossed in order to produce new rootstock genotypes. Immature embryos were taken from fruit after 80, 100 and 120 pollination days to determine the suitable embryo rescue stage. Then, the embryos were germinated in Murashige and Tucker (MT) culture medium including 0, 0.5 and 1 mg l-1 GA3. According to the results, high germination rates were materialized as 100% and on 95% to taken embryos from 120 days after pollination (DAP) in supplemented with 1 mg l-1 GA3 and 0.5 mg l-1 GA3 in MT culture medium respectively. The embryos taken from 80 days after pollination did not germinate on MT medium without GA3 (control) and including with 0.5 mg l-1. Generally, the existence of GA3 on medium increased the ratio of germination compared to the control. The highest trifoliate ratio was determined as 33.33% at the embryos taken from 100 and 120 days after pollination and medium including 0.5 and 1 mg l-1 GA3 consecutively. The results have revealed that the optimal time for embryo rescue at citrus is 120 DAP and including GA3 in the medium increased embryo germination.

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Plant regeneration via somatic embryogenesis from protoplasts of six plant species related to Citrus

Cited by 4 publications

References 0 publications

Cultivo de embriões imaturos de citros em diferentes concentrações de carvão ativado e ácido giberélico

Cultivo de embriões imaturos de citros em diferentes concentrações de carvão ativado e ácido giberélico

Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry

Yerli mandarin x Carrizo sitranjı melezlerinde, embriyo kurtarma tekniğinde embriyo gelişim aşaması ve GA3 dozlarının optimizasyonu

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