Plant Reproduction: Self-Incompatibility to Go

Tovar‐Méndez, Alejandro; McClure, Bruce

doi:10.1016/j.cub.2015.12.011

Cited by 6 publications

(6 citation statements)

References 17 publications

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“…Further, the industrial waste from Papaver plants contains highly volatile chemicals that are more feasible for the production of biofuels [ 22 ]. Furthermore, Papaver contributes its self-incompatibility molecular mechanisms to other plants [ 23 , 24 , 25 ], and it is used as a model plant for studying non-target site resistance to herbicides, which is one of the original problems in chemical weed control systems [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs

Shin

et al. 2018

IJMS

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The Papaver spp. (Papaver rhoeas (Corn poppy) and Papaver nudicaule (Iceland poppy)) genera are ornamental and medicinal plants that are used for the isolation of alkaloid drugs. In this study, we generated 700 Mb of transcriptome sequences with the PacBio platform. They were assembled into 120,926 contigs, and 1185 (82.2%) of the benchmarking universal single-copy orthologs (BUSCO) core genes were completely present in our assembled transcriptome. Furthermore, using 128 Gb of Illumina sequences, the transcript expression was assessed at three stages of Papaver plant development (30, 60, and 90 days), from which we identified 137 differentially expressed transcripts. Furthermore, three co-occurrence heat maps are generated from 51 different plant genomes along with the Papaver transcriptome, i.e., secondary metabolite biosynthesis, isoquinoline alkaloid biosynthesis (BIA) pathway, and cytochrome. Sixty-nine transcripts in the BIA pathway along with 22 different alkaloids (quantified with LC-QTOF-MS/MS) were mapped into the BIA KEGG map (map00950). Finally, we identified 39 full-length cytochrome transcripts and compared them with other genomes. Collectively, this transcriptome data, along with the expression and quantitative metabolite profiles, provides an initial recording of secondary metabolites and their expression related to Papaver plant development. Moreover, these profiles could help to further detail the functional characterization of the various secondary metabolite biosynthesis and Papaver plant development associated problems.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs

Shin

et al. 2018

IJMS

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“…Using this threshold, 17 regions were mapped, all of which are located on chromosome 2 (Table 3). 4 ) and the horizontal coordinate is the chromosome position. The colored scatter is the raw association value (ED 4 ) for each SNP, the black curve is the association value after sliding window fitting, and the red dashed line is the threshold line (0.27).…”

Section: Ed Correlation Analysismentioning

confidence: 99%

“…Self-incompatibility (SI) in plants is a fundamental recognition system that enables plants to distinguish between their own pollen and that of other individuals, thereby preventing the germination or growth of their own pollen or pollen from different plants of the same species on their stigma. SI is a prevalent phenomenon in flowering plants and serves to avoid inbreeding, promote outcrossing, and thereby enhance the survival and adaptability of offspring [4,5]. The investigation of SI originated in the 1870s when Darwin first observed and described the phenomenon [6].…”

Section: Introductionmentioning

confidence: 99%

“…Since the early 20th century, scientists have conducted field surveys and hybrid experiments to study SI, and interest in this research area has been continually growing. Currently, research into the molecular and biochemical mechanisms underlying SI recognition is largely focused on plants within five families: Solanaceae, Rosaceae, Scrophulariaceae, Papaveraceae, and Cruciferae [4,7,8].…”

Section: Introductionmentioning

confidence: 99%

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Localization of S-Locus-Related Self-Incompatibility in Lycium barbarum Based on BSA Analysis

Wang,

Wu,

Gao

et al. 2024

Horticulturae

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The recognition of pollen and pistil in the self-incompatibility process is generally determined by the interaction between the pollen S gene and pistil S gene located at the S locus. However, the regulatory mechanism of self-incompatibility in goji remains unknown. In this study, we used the self-compatible strain ‘13–19’ and self-incompatible strain ‘xin9’ from Ningxia as parents to create an F1 hybrid population. Reciprocal cross-pollination was performed within the same plant to evaluate the self-compatibility of the parents and F1 progeny. The parents and progeny were subjected to whole-genome resequencing, and mixed pools of DNA were constructed using 30 self-compatible and 30 self-incompatible individuals. Association analysis using the SNP-index method and Euclidean distance was employed to identify the key candidate region of the S locus. The candidate region was further annotated using the Swiss-Prot database to identify genes within the region. Additionally, transcriptome sequencing data from different organs/tissues, as well as from pistils of self-compatible and self-incompatible strains at control (0 h), short (0.5 h), medium (8 h), and long (48 h) time points after self-pollination and cross-pollination, were analyzed to assess differential gene expression and screen for self-compatibility-related loci. Specific primers were designed for PCR amplification to determine the S-RNase genotypes of the extreme parents. The results revealed that the S locus in goji is located within a 32.2 Mb region on chromosome 2 that contains a total of 108 annotated genes. Differential expression analysis showed that ten genes, including Lba02g01064, were specifically expressed in stamens, with four of them annotated as F-box genes, potentially serving as determinants of self-compatibility in stamens. Lba02g01102 was exclusively expressed in pistils and annotated as an S-RNase gene, likely involved in self-compatibility. The expression of Lba02g01102 in pistils decreased after self-pollination and cross-pollination. Six candidate genes exhibited significant changes after self-pollination and cross-pollination. Both parents and progeny carried two S-RNase alleles, and the S-RNase genotypes showed a significant correlation with self-compatibility, with the self-compatible progeny containing the S8-RNase allele. The identification of the S locus in goji provides molecular markers for future marker-assisted breeding and offers genetic resources for studying the mechanism of self-incompatibility in goji, thus contributing to the improvement of goji varieties.

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“…Thus, it is crucial for species' adaptation and evolution [18][19][20][21]. SI is widespread in angiosperm species, and it is estimated that 40-60% of all species of flowering plants present SI [3][4][5][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Breakdown of Self-Incompatibility in Citrus by Temperature Stress, Bud Pollination and Polyploidization

et al. 2022

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Self-incompatibility (SI) is present in around half of all species of flowering plants. SI limits endogamy and contributes to increased genetic diversity. SI is a very important trait in citrus because, when coupled with parthenocarpy, it allows seedless fruit production. Otherwise, SI is an impediment to genetic studies and breeding programs. Temperature stress, bud pollination and polyploidization can induce the breakdown of the SI mechanism in several species. In this work, we investigated how the SI mechanism can be broken down in two self-incompatible diploid citrus genotypes: ‘Fortune’ mandarin and ‘Clemenules’ clementine. The influence of temperature stress on the SI mechanism was assessed in self-pollinated flowers of ‘Fortune’ mandarins subjected to 2 temperature regimes (10 °C and 30 °C), whereas the bud pollination effect was investigated in the same genotype and in ‘Clemenules’ clementines cultivated under field conditions. The tetraploid ‘Clemenules’ clementine cultivated under field conditions was used to study if tetraploidization can bypass the SI reaction. Histological observations of pollen tube growth and seed production in self-pollinated flowers were used to evaluate the breakdown of SI, while the genetic analysis with SSR and SNP markers confirmed that all recovered plants were zygotic and had been originated by selfing. Our results confirm that the SI reaction can be surpassed by temperature stress, bud pollination and tetraploidy. To our knowledge, this is the first report in citrus in which the SI reaction breakdown by these three different strategies is demonstrated by molecular markers.

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Plant Reproduction: Self-Incompatibility to Go

Cited by 6 publications

References 17 publications

Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs

Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs

Localization of S-Locus-Related Self-Incompatibility in Lycium barbarum Based on BSA Analysis

Breakdown of Self-Incompatibility in Citrus by Temperature Stress, Bud Pollination and Polyploidization

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