Plant responses to tomato chlorotic mottle virus: Proteomic view of the resistance mechanisms to a bipartite begomovirus in tomato

Carmo, Lílian S.T.; Murad, André M.; Resende, Renato O.; Boiteux, L. S.; Ribeiro, S. G.; Jorrín‐Novo, Jesús V.; Mehta, Ângela

doi:10.1016/j.jprot.2016.07.018

Cited by 17 publications

(13 citation statements)

References 56 publications

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“…Both PTI and ETI are involved in early signaling events, including changes in protein phosphorylation status [ 39 ]. Some protein kinase and ATP binding activity-related transcripts were highly down-regulated in MIMV-infected maize ( Table 4 ) whereas some others were up-regulated as previously shown in tomato cultivars resistant to tomato chlorotic mottle virus [ 40 ]. Differential expression of those host genes appears to depend on the type of transcripts and their specific roles in cell signaling.…”

Section: Resultsmentioning

confidence: 53%

“…The important role of kinases in plant innate immunity is well known [ 41 ] and the response of maize to MIMV infection is no exception. GO classification of up-regulated transcripts in molecular function showed 158 transcripts that relate to nucleotide binding, 167 to hydrolase activity and 330 to heterocyclic compound binding that are well known responses in plant defense [ 40 , 42 ]. Transcripts that relate to nucleotide binding were also up-regulated in maize infected with maize chlorotic dwarf virus (MCDV) and the nucleorhabdovirus maize fine streak virus (MFSV) [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

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Changes in maize transcriptome in response to maize Iranian mosaic virus infection

2018

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BackgroundMaize Iranian mosaic virus (MIMV, genus Nucleorhabdovirus, family Rhabdoviridae) causes an economically important disease in maize and other gramineous crops in Iran. MIMV negative-sense RNA genome sequence of 12,426 nucleotides has recently been completed. Maize Genetics and Genomics database shows that 39,498 coding genes and 4,976 non-coding genes of maize have been determined, but still some transcripts could not be annotated. The molecular host cell responses of maize to MIMV infection including differential gene expression have so far not been elucidated.Methodology/Principal findingsComplementary DNA libraries were prepared from total RNA of MIMV-infected and mock-inoculated maize leaves and sequenced using Illumina HiSeq 2500. Cleaned raw transcript reads from MIMV-infected maize were mapped to reads from uninfected maize and to a maize reference genome. Differentially expressed transcripts were characterized by gene ontology and biochemical pathway analyses. Transcriptome data for selected genes were validated by real-time quantitative PCR.Conclusion/SignificanceApproximately 42 million clean reads for each treatment were obtained. In MIMV-infected maize compared to uninfected plants, 1689 transcripts were up-regulated and 213 transcripts were down-regulated. In response to MIMV infection, several pathways were activated in maize including immune receptor signaling, metabolic pathways, RNA silencing, hormone-mediated pathways, protein degradation, protein kinase and ATP binding activity, and fatty acid metabolism. Also, several transcripts including those encoding hydrophobic protein RCI2B, adenosylmethionine decarboxylase NAC transcription factor and nucleic acid binding, leucine-rich repeat, heat shock protein, 26S proteasome, oxidoreductases and endonuclease activity protein were up-regulated. These data will contribute to the identification of genes and pathways involved in plant-virus interactions that may serve as future targets for improved disease control.

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Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Changes in maize transcriptome in response to maize Iranian mosaic virus infection

2018

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“…Resequencing information was obtained using genomic DNA from the erectoid leaf line 'LT05' and from four genetically diverse wild-type leaf accessions: 'TO-937' (Powell et al 2012), 'CNPH498' (data not shown), 'Santa Clara' (Carmo et al, 2017), and 'Viradoro' (Giordano et al 2000). Genomic DNA was extracted from leaf tissue of these accessions using DNeasy PowerPlant Pro Kit (QIAGEN Hilden, Germany).…”

Section: Comparative Genomic Analyses Of the Chromosome 10 And Varianmentioning

confidence: 99%

A loss-of-function allele of a TAC1-like gene (SlTAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutation

et al. 2019

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The genetic basis of an erectoid leaf phenotype was investigated in distinct tomato breeding populations, including one derived from Solanum lycopersicum 'LT05' (with the erectoid leaf phenotype and uniform ripening, genotype uu) × S. pimpinelifollium 'TO-937' (with the wild-type leaf phenotype and green fruit shoulder, genotype UU). The erectoid leaf phenotype was inherited as a semi-dominant trait and it co-segregated with the u allele of gene SlGLK2 (Solyc10g008160). This genomic location coincides with a previously described semi-dominant mutation named as Erectoid leaf (Erl). The genomes of 'LT05', 'TO-937', and three other unrelated accessions (with the wild-type Erl + allele) were resequenced with the aim of identifying candidate genes. Comparative genomic analyses, including the reference genome 'Heinz 1706' (Erl + allele), identified an Erectoid leaf-specific single nucleotide polymorphism (SNP) in the gene Solyc10g009320. This SNP caused a change of a glutamine (CAA) codon (present in all the wild-type genomes) to a TAA (= ochre stop-codon) in the Erl allele, resulting in a smaller version of the predicted mutant protein (221 versus 279 amino acids). Solyc10g009320, previously annotated as an 'unknown protein', was identified as a TILLER ANGLE CONTROL1 (TAC1)-like gene. Linkage between the Erl and Solyc10g009320 was confirmed via Sanger sequencing of the PCR amplicons of the two variant alleles. No recombinants were detected in 265 F2 individuals. Contrasting S7 nearisogenic lines were also homozygous for each of the alternate alleles, reinforcing Solyc10g009320 as a strong Erl candidate gene and opening the possibility for fine-tuning manipulation of tomato architecture in breeding programs.

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“…In addition, infectious clones can also be used to study the host response at a molecular level. For example, Tomato chlorotic mottle virus infectious clones were used to compare the proteomes of resistant and susceptible near‐isogenic tomato lines following infection (Carmo et al ., ).…”

Section: Introductionmentioning

confidence: 97%

A guide to the contained use of plant virus infectious clones

Brewer

Hird

Bailey

et al. 2018

Plant Biotechnology Journal

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Plant virus infectious clones are important tools with wide‐ranging applications in different areas of biology and medicine. Their uses in plant pathology include the study of plant–virus interactions, and screening of germplasm as part of prebreeding programmes for virus resistance. They can also be modified to induce transient plant gene silencing (Virus Induced Gene Silencing – VIGS) and as expression vectors for plant or exogenous proteins, with applications in both plant pathology and more generally for the study of plant gene function. Plant viruses are also increasingly being investigated as expression vectors for in planta production of pharmaceutical products, known as molecular farming. However, plant virus infectious clones may pose a risk to the environment due to their ability to reconstitute fully functional, transmissible viruses. These risks arise from both their inherent pathogenicity and the effect of any introduced genetic modifications. Effective containment measures are therefore required. There has been no single comprehensive review of the biosafety considerations for the contained use of genetically modified plant viruses, despite their increasing importance across many biological fields. This review therefore explores the biosafety considerations for working with genetically modified plant viruses in contained environments, with focus on plant growth facilities. It includes regulatory frameworks, risk assessment, assignment of biosafety levels, facility features and working practices. The review is based on international guidance together with information provided by plant virus researchers.

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Plant responses to tomato chlorotic mottle virus: Proteomic view of the resistance mechanisms to a bipartite begomovirus in tomato

Cited by 17 publications

References 56 publications

Changes in maize transcriptome in response to maize Iranian mosaic virus infection

Changes in maize transcriptome in response to maize Iranian mosaic virus infection

A loss-of-function allele of a TAC1-like gene (SlTAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutation

A guide to the contained use of plant virus infectious clones

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