Plant single-cell and single-cell-type metabolomics

Misra, Biswapriya B.; Assmann, Sarah M.; Chen, Sixue

doi:10.1016/j.tplants.2014.05.005

Cited by 109 publications

(88 citation statements)

References 89 publications

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“…For transitions that require complex changes in different tissues and cells of an organ (e.g. seed development or branching), it is necessary to investigate signaling processes at high temporal and spatial resolution, for example, using biosensors (Jones et la., 2013) or single cell analysis of metabolites, transcripts, and protein after laser microdissection (Misra et al, 2014). Given that developmental transitions are often linked, developmental changes triggered by sugars early in development may have later consequences (e.g.…”

Section: Discussionmentioning

confidence: 99%

Transitioning to the Next Phase: The Role of Sugar Signaling throughout the Plant Life Cycle

Wingler

2017

Plant Physiol.

140

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Section: Discussionmentioning

confidence: 99%

Transitioning to the Next Phase: The Role of Sugar Signaling throughout the Plant Life Cycle

Wingler

2017

Plant Physiol.

140

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“…A major bottleneck and challenge in plant cell‐specific studies, however, is in obtaining different cell types in high enough quantity and quality for downstream analysis (Misra et al . ). Nevertheless, several elegant studies involving the parallel usage of different high‐throughput technologies have employed guard cells.…”

Section: Introductionmentioning

confidence: 97%

Utilizing systems biology to unravel stomatal function and the hierarchies underpinning its control

Medeiros

Daloso

Fernie

et al. 2015

Plant Cell & Environment

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Stomata control the concomitant exchange of CO2 and transpiration in land plants. While a constant supply of CO2 is need to maintain the rate of photosynthesis, the accompanying water losses must be tightly regulated to prevent dehydration and undesired metabolic changes. The factors affecting stomatal movement are directly coupled with the cellular networks of guard cells. Although the guard cell has been used as a model for characterization of signaling pathways, several important questions about its functioning remain elusive. Current modeling approaches describe the stomatal conductance in terms of relatively few easy‐to‐measure variables being unsuitable for in silico design of genetic manipulation strategies. Here, we argue that a system biology approach, combining modeling and high‐throughput experiments, may be used to elucidate the mechanisms underlying stomata control and to determine targets for modulation of stomatal responses to environment. In support of our opinion, we review studies demonstrating how high‐throughput approaches have provided a systems‐view of guard cells. Finally, we emphasize the opportunities and challenges of genome‐scale modeling and large‐scale data integration for in silico manipulation of guard cell functions to improve crop yields, particularly under stress conditions which are of pertinence both to climate change and water use efficiency.

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“…Because they are essential for plant physiology and are present on all large land plants, stomata have been the subject of studies ranging from probes of single molecules to global scale ecophysiology. As a consequence of the wide-scale interest in stomatal properties, mature guard cell transcriptomes, proteomes and metabolomes have been generated and stomatal activities modeled (Misra et al, 2014; Yang et al, 2008; Zhao et al, 2008). Because of increasing interest and progress elucidating the integration of environmental cues (such as light and carbon dioxide) with endogenous circuits to control stomatal production and activity (e.g., Casson and Hetherington, 2014; Engineer et al, 2014), transcriptional profiles of developing GCs or their precursors would be invaluable community resources.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Dynamics of the Stomatal Lineage: Birth, Amplification, and Termination of a Self-Renewing Population

et al. 2015

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Summary Developmental transitions can be described in terms of morphology and the roles of individual genes, but also in terms of global transcriptional and epigenetic changes. Temporal dissections of transcriptome changes, however, are rare for intact, developing tissues. We used RNA sequencing and microarray platforms to quantify gene expression from labeled cells isolated by Fluorescence Activated Cell Sorting to generate cell-type specific transcriptomes during development of an adult stem-cell lineage in the Arabidopsis leaf. We show regulatory modules in this early lineage link cell types that had previously been considered to be under separate control and provide evidence for recruitment of individual members of gene families for different developmental decisions. Because stomata are physiologically important and because stomatal lineage cells exhibit exemplary division, cell fate and cell signaling behaviors, this dataset serves as a valuable resource for further investigations of fundamental developmental processes.

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Plant single-cell and single-cell-type metabolomics

Cited by 109 publications

References 89 publications

Transitioning to the Next Phase: The Role of Sugar Signaling throughout the Plant Life Cycle

Transitioning to the Next Phase: The Role of Sugar Signaling throughout the Plant Life Cycle

Utilizing systems biology to unravel stomatal function and the hierarchies underpinning its control

Transcriptome Dynamics of the Stomatal Lineage: Birth, Amplification, and Termination of a Self-Renewing Population

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