Plant Structure

Bowes, Bryan G.; Mauseth, James D.

doi:10.1201/b15143

Cited by 17 publications

(9 citation statements)

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“…The surface view shows that both in vivo ( Figure 5(a) ) and in vitro ( Figure 5(b) ) C. indica leaves possess paracytic stomata, characterized by the position of the subsidiary cell which is parallel to the guard cells [ 40 ]. It has typical monocot stomata in which the dumbbell-shape guard cells with unevenly thickened walls are dwarfed by the larger subsidiary cells, which are lacking in dicot stomata [ 41 ]. Furthermore, the subsidiary cells and the long axes of the stomata lie parallel to each other.…”

Section: Resultsmentioning

confidence: 99%

Organogenesis and Ultrastructural Features ofIn VitroGrownCanna indicaL.

Wafa

Taha

Mohajer

et al. 2016

BioMed Research International

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An efficient protocol for micropropagation of Canna indica L., an economically and pharmaceutically important plant, was standardized using rhizome explants, excised from two-month-old aseptic seedlings. Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%) and roots (86.7 ± 0.4%) after 2 weeks. Furthermore, the optimum media for multiple shoots regeneration were recorded on MS enriched with 7.0 mg/L BAP (33.0 ± 0.5%). Plantlets obtained were transplanted to pots after two months and acclimatized in the greenhouse, with 75% survival. In addition, ultrastructural studies showed that rhizomes of in vitro grown specimens were underdeveloped compared to the in vivo specimens, possibly due to the presence of wide spaces. Meanwhile, the leaves of in vivo specimens had more open stomata compared to in vitro specimens, yet their paracytic stomata structures were similar. Hence, there were no abnormalities or major differences between in vitro regenerants and mother plants.

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Section: Resultsmentioning

confidence: 99%

Organogenesis and Ultrastructural Features ofIn VitroGrownCanna indicaL.

Wafa

Taha

Mohajer

et al. 2016

BioMed Research International

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“…In higher plants, except few plants, such as tobacco, it was long believed that no chloroplasts exist in epidermal cells other than guard cells [ 23 , 24 , 25 , 26 ]. Most studies on chloroplast focus on mesophyll cells, where numerous typical large chloroplasts are highly differentiated for photosynthesis [ 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Emerging Roles of Motile Epidermal Chloroplasts in Plant Immunity

Irieda

2022

IJMS

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Plant epidermis contains atypical small chloroplasts. However, the physiological role of this organelle is unclear compared to that of large mesophyll chloroplasts, the well-known function of which is photosynthesis. Although knowledge of the involvement of chloroplasts in the plant immunity has been expanded to date, the differences between the epidermal and mesophyll chloroplasts are beyond the scope of this study. Given the role of the plant epidermis as a barrier to environmental stresses, including pathogen attacks, and the immune-related function of chloroplasts, plant defense research on epidermal chloroplasts is an emerging field. Recent studies have revealed the dynamic movements of epidermal chloroplasts in response to fungal and oomycete pathogens. Furthermore, epidermal chloroplast-associated proteins and cellular events that are tightly linked to epidermal resistance against pathogens have been reported. In this review, I have focused on the recent progress in epidermal chloroplast-mediated plant immunity.

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“…Although for certain groups of plants such morphological data can be easily obtained, for many others this is not a simple task. Plant tissues may commonly possess characteristics that are prohibitive to preservation and examination by microscopy, including waxy and/or pubescent (hairy) surfaces, thick cuticles, thick lignified or cellulosic cell walls, highly vacuolated cells, and intracellular crystals (Bowes & Mauseth, 2008). Such features require adaptive approaches to sample processing (Kuo, 2007; Bowes & Mauseth, 2008) and these can often be further improved with the use of microwave techniques (Giberson & Demaree, 2001; Zechmann & Zellnig, 2009).…”

Section: Introductionmentioning

confidence: 99%

A Method for Preparing Difficult Plant Tissues for Light and Electron Microscopy

Clode

2015

Microsc Microanal

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Although the advent of microwave technologies has both improved and accelerated tissue processing for microscopy, there still remain many limitations in conventional chemical fixation, dehydration, embedding, and sectioning, particularly with regard to plant materials. The Proteaceae, a family of plants widely distributed in the Southern Hemisphere and well adapted to harsh climates and nutrient-poor soils, is a perfect example; the complexity of Proteaceae leaves means that almost no ultrastructural data are available as these are notoriously difficult to both infiltrate and section. Here, a step-by-step protocol is described that allows for the successful preparation of Banksia prionotes (Australian Proteaceae) leaves for both light and transmission electron microscopy. The method, which applies a novel combination of vibratome sectioning, microwave processing and vacuum steps, and the utilization of an ultra low viscosity resin, results in highly reproducible, well-preserved, sectionable material from which very high-quality light and electron micrographs can be obtained. With this, cellular ultrastructure from the level of a leaf through to organelle substructure can be studied. This approach will be widely applicable, both within and outside of the plant sciences, and can be readily adapted to meet specific sample requirements and imaging needs.

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Plant Structure

Cited by 17 publications

References 0 publications

Organogenesis and Ultrastructural Features ofIn VitroGrownCanna indicaL.

Organogenesis and Ultrastructural Features ofIn VitroGrownCanna indicaL.

Emerging Roles of Motile Epidermal Chloroplasts in Plant Immunity

A Method for Preparing Difficult Plant Tissues for Light and Electron Microscopy

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